Cellulomonas hominis
Taxonomy:
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4041 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A511F851|A0A511F851_9CELL DNA-binding transcriptional MerR regulator OS=Cellulomonas hominis OX=156981 GN=CHO01_05010 PE=4 SV=1
MM1 pKa = 7.57 PAGPAVAAPADD12 pKa = 3.99 DD13 pKa = 4.24 CAAPTRR19 pKa = 11.84 TVTGSTSGAPLDD31 pKa = 3.82 VAAGEE36 pKa = 4.44 VVLIAGGTQSGGVNSLPAGATLCVAAGATLAPAYY70 pKa = 10.21 VNNAAGTVYY79 pKa = 10.3 VAPGGTASLPSIATNAGFVLDD100 pKa = 4.03 NAGTTTVAGLNVNGPGTVRR119 pKa = 11.84 NAAGATLAVTGQLAPAAGTVVNDD142 pKa = 3.9 GTLALPGGATVNGNVSLVNNGTLTAGGTTTISGAFRR178 pKa = 11.84 NAGTATLGGDD188 pKa = 3.59 VIVNGGGSLHH198 pKa = 6.16 NLCIIDD204 pKa = 3.8 AAGGLTTNAPGSTNTGLVALGGGLQVSGSGSWSQTPTGATTATTLTDD251 pKa = 3.84 DD252 pKa = 4.52 GAVTGYY258 pKa = 10.57 GRR260 pKa = 11.84 YY261 pKa = 9.98 AFTGTTSVQGTFAGDD276 pKa = 3.36 LALVPIFLDD285 pKa = 3.65 VPGTTFPYY293 pKa = 10.02 FDD295 pKa = 3.7 VVNGTVANVDD305 pKa = 3.52 RR306 pKa = 11.84 RR307 pKa = 11.84 DD308 pKa = 3.58 LTIPGPADD316 pKa = 3.62 YY317 pKa = 8.01 PAPDD321 pKa = 4.47 CADD324 pKa = 3.64 PAAPPSADD332 pKa = 3.28 VQVAKK337 pKa = 9.99 TGPASVLPDD346 pKa = 3.22 GTLTYY351 pKa = 10.42 TVTVTNAGPDD361 pKa = 3.28 AAEE364 pKa = 4.64 DD365 pKa = 3.73 VTVTDD370 pKa = 4.11 TLPPTLLDD378 pKa = 3.77 PAADD382 pKa = 3.51 AGGVVAGGTVTWSLGTLAAGQTVTLTASGRR412 pKa = 11.84 APADD416 pKa = 3.34 GTLVNVVSATSTTPDD431 pKa = 3.63 PDD433 pKa = 3.23 ASNNDD438 pKa = 2.82 GSADD442 pKa = 3.88 SEE444 pKa = 4.82 TVTTDD449 pKa = 3.34 VEE451 pKa = 4.36 PSPPDD456 pKa = 3.38 TGPPTAAPLTRR467 pKa = 11.84 DD468 pKa = 4.08 GPPGLPIVGVLDD480 pKa = 4.43 GSSPDD485 pKa = 3.61 PDD487 pKa = 3.32 LQLRR491 pKa = 11.84 YY492 pKa = 10.03 SVVDD496 pKa = 4.07 GPTSGRR502 pKa = 11.84 VIALPNGLFGYY513 pKa = 10.4 LPDD516 pKa = 5.26 DD517 pKa = 4.24 GFVGTDD523 pKa = 2.74 TFTYY527 pKa = 9.86 RR528 pKa = 11.84 VCDD531 pKa = 3.44 NQTPAEE537 pKa = 4.3 CSAPATVTLIIHH549 pKa = 6.33 PRR551 pKa = 11.84 AVNDD555 pKa = 3.4 EE556 pKa = 4.37 TTTLMDD562 pKa = 3.3 QPVTIDD568 pKa = 3.58 VLANDD573 pKa = 4.24 PGGAVLSSNLDD584 pKa = 3.16 ASASHH589 pKa = 6.9 GLTQIDD595 pKa = 4.53 PATGTVTYY603 pKa = 9.7 TPGPGYY609 pKa = 10.62 LGDD612 pKa = 3.56 DD613 pKa = 3.6 TFVYY617 pKa = 9.91 RR618 pKa = 11.84 ACAPADD624 pKa = 4.07 PGDD627 pKa = 3.86 CALAVVVVHH636 pKa = 5.99 VVPDD640 pKa = 4.03 NLPPLAPPLAMEE652 pKa = 4.46 TTVGAPVTGAPAVSDD667 pKa = 3.96 PDD669 pKa = 3.64 GDD671 pKa = 4.09 AVALASVFPPTTGDD685 pKa = 3.07 ATATTTTTTYY695 pKa = 9.94 TPPPGFAGRR704 pKa = 11.84 AVYY707 pKa = 10.09 QYY709 pKa = 7.55 TVCDD713 pKa = 3.87 DD714 pKa = 4.1 GVPVLCATGLVTVLVDD730 pKa = 4.1 PVAGDD735 pKa = 4.24 DD736 pKa = 4.63 SATTPAGTPVTVDD749 pKa = 2.91 VAANDD754 pKa = 4.08 LGTVLPPDD762 pKa = 3.85 VATPPANGTVALAGGALVYY781 pKa = 10.12 TPAPGFVGTDD791 pKa = 2.81 TFTYY795 pKa = 9.78 RR796 pKa = 11.84 ICADD800 pKa = 4.1 DD801 pKa = 4.12 VSPACDD807 pKa = 2.98 TATVTVVVTGDD818 pKa = 4.11 GPPVDD823 pKa = 4.98 PGDD826 pKa = 4.52 PGDD829 pKa = 4.34 GGAGGTGGGSGTGAAPGGGGLPVTGADD856 pKa = 3.74 AAPLAALAAALLAAGAAALTARR878 pKa = 11.84 RR879 pKa = 11.84 RR880 pKa = 11.84 ATAPARR886 pKa = 11.84 ARR888 pKa = 11.84 RR889 pKa = 11.84 PGPRR893 pKa = 11.84 RR894 pKa = 3.3
Molecular weight: 86.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.579
IPC_protein 3.656
Toseland 3.389
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.668
Rodwell 3.465
Grimsley 3.3
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.139
Thurlkill 3.465
EMBOSS 3.668
Sillero 3.783
Patrickios 0.757
IPC_peptide 3.656
IPC2_peptide 3.745
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A511F8J5|A0A511F8J5_9CELL AcrR family transcriptional regulator OS=Cellulomonas hominis OX=156981 GN=CHO01_06880 PE=4 SV=1
MM1 pKa = 7.39 GRR3 pKa = 11.84 RR4 pKa = 11.84 GSVRR8 pKa = 11.84 LWGGVAFAALVLVAVGWAFAPAITLLVGVVVAALAVPVGIVLVATAALSRR58 pKa = 11.84 RR59 pKa = 3.97
Molecular weight: 5.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.387
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.115
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4041
0
4041
1292075
39
2005
319.7
33.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.344 ± 0.068
0.559 ± 0.009
6.441 ± 0.036
5.19 ± 0.036
2.434 ± 0.022
9.555 ± 0.037
2.019 ± 0.017
2.766 ± 0.029
1.277 ± 0.023
10.415 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.574 ± 0.014
1.383 ± 0.016
6.213 ± 0.033
2.614 ± 0.021
8.1 ± 0.044
4.591 ± 0.031
6.121 ± 0.044
10.043 ± 0.04
1.556 ± 0.018
1.805 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here