Pseudomonas phage oldone

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Bruynoghevirus; unclassified Bruynoghevirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G9LRQ5|A0A6G9LRQ5_9CAUD Uncharacterized protein OS=Pseudomonas phage oldone OX=2719607 GN=oldone_22 PE=4 SV=1
MM1 pKa = 7.5IAVLDD6 pKa = 4.7DD7 pKa = 3.51GTVYY11 pKa = 10.67VPWRR15 pKa = 11.84YY16 pKa = 9.41HH17 pKa = 7.29LEE19 pKa = 3.9SLYY22 pKa = 11.71NMASAGGATDD32 pKa = 3.85PEE34 pKa = 4.37AVKK37 pKa = 11.11GEE39 pKa = 3.91FLDD42 pKa = 3.9WVRR45 pKa = 11.84SEE47 pKa = 3.81YY48 pKa = 10.84GRR50 pKa = 11.84YY51 pKa = 7.18VTIEE55 pKa = 3.96LEE57 pKa = 4.63GIPEE61 pKa = 4.11DD62 pKa = 4.75RR63 pKa = 11.84EE64 pKa = 4.22DD65 pKa = 4.82AFDD68 pKa = 3.92EE69 pKa = 4.47YY70 pKa = 11.53VLKK73 pKa = 11.02GLL75 pKa = 4.61

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G9LNP2|A0A6G9LNP2_9CAUD Uncharacterized protein OS=Pseudomonas phage oldone OX=2719607 GN=oldone_13 PE=4 SV=1
MM1 pKa = 7.7GKK3 pKa = 9.85RR4 pKa = 11.84KK5 pKa = 9.69VFYY8 pKa = 10.55EE9 pKa = 4.02RR10 pKa = 11.84LCSNGEE16 pKa = 3.75LTLNGNLKK24 pKa = 8.43ATKK27 pKa = 10.24VSAPTKK33 pKa = 10.02GKK35 pKa = 6.55QQRR38 pKa = 11.84RR39 pKa = 11.84GTFF42 pKa = 2.94

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13891

31

1055

198.4

22.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.437 ± 0.593

1.037 ± 0.137

6.04 ± 0.234

7.141 ± 0.465

3.794 ± 0.233

8.401 ± 0.342

2.095 ± 0.186

5.255 ± 0.26

5.68 ± 0.297

8.2 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.162

4.175 ± 0.256

4.255 ± 0.207

3.981 ± 0.283

5.889 ± 0.264

5.73 ± 0.242

5.147 ± 0.287

7.113 ± 0.225

1.62 ± 0.136

3.268 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski