Cellulophaga phage phi12a:1

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 8.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A338|S0A338_9VIRU Replication initiation factor OS=Cellulophaga phage phi12a:1 OX=1327987 GN=Phi12a:1_pg2 PE=4 SV=1
MM1 pKa = 6.9VVGYY5 pKa = 8.65FIGVNRR11 pKa = 11.84DD12 pKa = 3.22FKK14 pKa = 11.19LIEE17 pKa = 4.14IPVLFEE23 pKa = 4.11NEE25 pKa = 3.64IFSTKK30 pKa = 9.86EE31 pKa = 3.37FFEE34 pKa = 4.69DD35 pKa = 3.67VLLFDD40 pKa = 5.88LFINQQRR47 pKa = 11.84YY48 pKa = 7.89FYY50 pKa = 10.42RR51 pKa = 11.84GFNAAEE57 pKa = 3.57THH59 pKa = 6.1AFNNTLSVEE68 pKa = 4.87LIQEE72 pKa = 4.15RR73 pKa = 11.84YY74 pKa = 9.11FRR76 pKa = 11.84SEE78 pKa = 3.88GQKK81 pKa = 10.78LEE83 pKa = 4.21FNMEE87 pKa = 4.17YY88 pKa = 10.08IFRR91 pKa = 11.84LSCCDD96 pKa = 3.25FTSRR100 pKa = 11.84IPSRR104 pKa = 11.84SEE106 pKa = 3.46LALPDD111 pKa = 3.98YY112 pKa = 11.29DD113 pKa = 4.49PLFTFNVVNGPNSNYY128 pKa = 9.28PLHH131 pKa = 6.33NFAYY135 pKa = 10.07GLYY138 pKa = 8.39STGGQFIPTIIEE150 pKa = 3.96DD151 pKa = 4.47FKK153 pKa = 11.63ADD155 pKa = 3.46TNTVVWQYY163 pKa = 11.37RR164 pKa = 11.84LSDD167 pKa = 3.69VVGKK171 pKa = 9.86EE172 pKa = 3.38IAFIAFDD179 pKa = 4.35PNGSNVFTLEE189 pKa = 3.85VDD191 pKa = 4.23GVQYY195 pKa = 10.87AQEE198 pKa = 4.3GYY200 pKa = 9.79IYY202 pKa = 11.01VFGSQSADD210 pKa = 3.22VVKK213 pKa = 9.7TVIINQTLQQ222 pKa = 2.93

Molecular weight: 25.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A3Q7|S0A3Q7_9VIRU Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Cellulophaga phage phi12a:1 OX=1327987 GN=Phi12a:1_pg8 PE=4 SV=1
MM1 pKa = 8.24RR2 pKa = 11.84ITNKK6 pKa = 9.86QLAVYY11 pKa = 9.26IGVSEE16 pKa = 4.2RR17 pKa = 11.84VVKK20 pKa = 10.41RR21 pKa = 11.84HH22 pKa = 4.98RR23 pKa = 11.84LYY25 pKa = 11.13YY26 pKa = 10.16ISCLQLKK33 pKa = 10.17RR34 pKa = 11.84NYY36 pKa = 8.49LTLYY40 pKa = 10.4DD41 pKa = 3.91ISRR44 pKa = 11.84LDD46 pKa = 3.53GVPVVVVARR55 pKa = 11.84IMGFPNSIINKK66 pKa = 8.61ISEE69 pKa = 4.31DD70 pKa = 3.36NGRR73 pKa = 3.65

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

1997

40

378

153.6

17.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.208 ± 0.745

1.052 ± 0.321

4.457 ± 0.452

4.607 ± 0.588

5.608 ± 0.966

5.709 ± 0.653

1.703 ± 0.481

7.712 ± 0.549

9.214 ± 1.019

9.664 ± 0.593

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.313

6.86 ± 0.545

2.303 ± 0.268

3.605 ± 0.507

4.206 ± 0.485

6.96 ± 0.4

6.159 ± 0.461

6.81 ± 0.689

1.352 ± 0.365

4.457 ± 0.481
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski