Streptococcus phage Javan310

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AY07|A0A4D6AY07_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan310 OX=2548102 GN=Javan310_0004 PE=4 SV=1
MM1 pKa = 7.34ATLNDD6 pKa = 3.26ILGYY10 pKa = 10.92AEE12 pKa = 4.39GLADD16 pKa = 4.19AGTGVSMSQWGMQCAALPNAISTYY40 pKa = 10.48FFGKK44 pKa = 7.55TLWGNAIDD52 pKa = 4.71LLNSARR58 pKa = 11.84DD59 pKa = 3.28LGYY62 pKa = 10.2EE63 pKa = 3.77VEE65 pKa = 4.76YY66 pKa = 10.8NQEE69 pKa = 4.09GNLDD73 pKa = 3.77SKK75 pKa = 10.74PRR77 pKa = 11.84AGAVFVMDD85 pKa = 3.45TTYY88 pKa = 10.46IYY90 pKa = 10.64GHH92 pKa = 6.28SYY94 pKa = 9.95GHH96 pKa = 6.54TGLVIEE102 pKa = 5.45DD103 pKa = 3.4SDD105 pKa = 4.88GYY107 pKa = 8.63TMRR110 pKa = 11.84TIEE113 pKa = 4.63QNIDD117 pKa = 2.96GNADD121 pKa = 3.3SLYY124 pKa = 9.74VGGPARR130 pKa = 11.84YY131 pKa = 7.49NTRR134 pKa = 11.84DD135 pKa = 3.3FNGIVGWFYY144 pKa = 11.12FPVDD148 pKa = 3.74GQPAQVTNVEE158 pKa = 4.03PSEE161 pKa = 4.08PLTVDD166 pKa = 2.85SSKK169 pKa = 11.08FNDD172 pKa = 3.34EE173 pKa = 4.04TGTFTVEE180 pKa = 3.56VSALNVRR187 pKa = 11.84SSAGLSGEE195 pKa = 3.74IVAVYY200 pKa = 8.6TAGQTINYY208 pKa = 8.74DD209 pKa = 2.88GWIDD213 pKa = 3.46NDD215 pKa = 4.04GYY217 pKa = 10.96IWITYY222 pKa = 8.93IAGSGNRR229 pKa = 11.84RR230 pKa = 11.84YY231 pKa = 10.23VAVGQSEE238 pKa = 4.13NGSRR242 pKa = 11.84INSFGSFAA250 pKa = 4.24

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AXY1|A0A4D6AXY1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan310 OX=2548102 GN=Javan310_0018 PE=4 SV=1
MM1 pKa = 7.35LAEE4 pKa = 4.25ILVGVLIIVVLFQMIIISSISEE26 pKa = 3.81RR27 pKa = 11.84CKK29 pKa = 9.61EE30 pKa = 4.33SKK32 pKa = 10.52RR33 pKa = 11.84EE34 pKa = 3.97LKK36 pKa = 10.84KK37 pKa = 10.58MIAEE41 pKa = 3.89QQRR44 pKa = 11.84IQEE47 pKa = 4.06ARR49 pKa = 11.84EE50 pKa = 3.75AMRR53 pKa = 11.84FGYY56 pKa = 10.13RR57 pKa = 11.84RR58 pKa = 3.33

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11162

41

1443

214.7

24.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.096 ± 0.654

0.502 ± 0.092

6.415 ± 0.306

8.099 ± 0.367

4.471 ± 0.339

6.441 ± 0.465

1.254 ± 0.1

6.692 ± 0.273

8.583 ± 0.357

8.61 ± 0.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.14

5.832 ± 0.234

2.643 ± 0.136

4.327 ± 0.232

4.005 ± 0.259

6.262 ± 0.27

6.038 ± 0.289

5.761 ± 0.278

1.156 ± 0.116

3.261 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski