Thalassoglobus polymorphus
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4824 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A517QGZ1|A0A517QGZ1_9PLAN Type I restriction enzyme EcoKI subunit R OS=Thalassoglobus polymorphus OX=2527994 GN=Mal48_01350 PE=4 SV=1
MM1 pKa = 7.25 NALFNSLLNDD11 pKa = 3.26 EE12 pKa = 5.13 AGFIVSAEE20 pKa = 4.14 LVLVATIVVLGMIVGLSEE38 pKa = 4.54 LALNINNEE46 pKa = 4.07 LEE48 pKa = 4.43 DD49 pKa = 3.46 VGSAFSGIQQSYY61 pKa = 7.08 EE62 pKa = 3.64 VRR64 pKa = 11.84 GMQGHH69 pKa = 6.69 KK70 pKa = 10.55 GWVDD74 pKa = 3.18 CSDD77 pKa = 4.12 FYY79 pKa = 11.64 DD80 pKa = 3.95 TVDD83 pKa = 4.35 FCAGQFDD90 pKa = 4.28 VQQ92 pKa = 3.6
Molecular weight: 9.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.681
IPC_protein 3.579
Toseland 3.401
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.554
Rodwell 3.427
Grimsley 3.325
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.897
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.719
Patrickios 0.604
IPC_peptide 3.541
IPC2_peptide 3.681
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|A0A517QM15|A0A517QM15_9PLAN SCP domain-containing protein OS=Thalassoglobus polymorphus OX=2527994 GN=Mal48_18440 PE=4 SV=1
MM1 pKa = 7.53 SSFRR5 pKa = 11.84 LPVNRR10 pKa = 11.84 EE11 pKa = 3.35 LWSASRR17 pKa = 11.84 LSGFTSPQDD26 pKa = 3.26 LVRR29 pKa = 11.84 ARR31 pKa = 11.84 EE32 pKa = 4.05 VVFLLFAGVCAALASGLFDD51 pKa = 4.26 FSLRR55 pKa = 11.84 IPGHH59 pKa = 6.23 AILRR63 pKa = 11.84 VIFPMTLGLALVPRR77 pKa = 11.84 KK78 pKa = 9.87 GAGTVMAAGAFSTGLFLRR96 pKa = 11.84 GTGIVAGMSIGAFTSLLVTGPLLDD120 pKa = 3.5 LTLRR124 pKa = 11.84 KK125 pKa = 8.02 TKK127 pKa = 10.14 NRR129 pKa = 11.84 SWIYY133 pKa = 10.15 LNCALAGLLSNSLAMSVRR151 pKa = 11.84 GLSKK155 pKa = 10.07 FLGWEE160 pKa = 3.64 RR161 pKa = 11.84 LGRR164 pKa = 11.84 VPVGEE169 pKa = 4.1 WFSRR173 pKa = 11.84 AVLTYY178 pKa = 8.04 PVCGLIAGLLCAMILFSMRR197 pKa = 11.84 VTEE200 pKa = 4.17 PEE202 pKa = 4.12 SVKK205 pKa = 11.02 GEE207 pKa = 4.05 EE208 pKa = 4.03
Molecular weight: 22.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.414
IPC_protein 10.072
Toseland 10.526
ProMoST 10.204
Dawson 10.613
Bjellqvist 10.35
Wikipedia 10.818
Rodwell 10.774
Grimsley 10.657
Solomon 10.745
Lehninger 10.716
Nozaki 10.555
DTASelect 10.321
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.54
IPC_peptide 10.745
IPC2_peptide 9.648
IPC2.peptide.svr19 8.509
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4824
0
4824
1776901
29
4052
368.3
40.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.321 ± 0.033
1.116 ± 0.015
5.697 ± 0.031
7.0 ± 0.039
4.051 ± 0.023
7.283 ± 0.04
2.234 ± 0.017
5.554 ± 0.024
4.717 ± 0.028
9.828 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.203 ± 0.016
3.66 ± 0.027
4.95 ± 0.032
4.332 ± 0.028
5.791 ± 0.032
6.846 ± 0.029
5.536 ± 0.028
6.952 ± 0.031
1.456 ± 0.015
2.474 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here