Cellulophaga phage phi14:2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A2D3|S0A2D3_9CAUD Uncharacterized protein OS=Cellulophaga phage phi14:2 OX=1327990 GN=Phi14:2_gp087 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.11QPCPMCMGSKK12 pKa = 10.28EE13 pKa = 4.07IFTGSKK19 pKa = 10.31VEE21 pKa = 4.2TCNTCDD27 pKa = 4.15DD28 pKa = 5.03DD29 pKa = 6.18GNVTQEE35 pKa = 3.76FLDD38 pKa = 3.67IVEE41 pKa = 5.15HH42 pKa = 5.99NDD44 pKa = 3.11EE45 pKa = 4.46YY46 pKa = 11.4EE47 pKa = 4.11GDD49 pKa = 3.66FF50 pKa = 4.51

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A375|S0A375_9CAUD Uncharacterized protein OS=Cellulophaga phage phi14:2 OX=1327990 GN=Phi14:2_gp019 PE=4 SV=1
MM1 pKa = 6.63THH3 pKa = 6.11WRR5 pKa = 11.84DD6 pKa = 3.4SGVGGIRR13 pKa = 11.84TPVTQIKK20 pKa = 10.27LIIILYY26 pKa = 9.82SFTFVYY32 pKa = 9.71TLYY35 pKa = 11.13ARR37 pKa = 11.84FIIKK41 pKa = 10.05KK42 pKa = 9.79

Molecular weight: 4.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

133

0

133

31995

33

2180

240.6

27.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.588 ± 0.19

0.85 ± 0.117

6.623 ± 0.12

7.064 ± 0.257

4.607 ± 0.12

5.798 ± 0.272

1.513 ± 0.09

8.051 ± 0.221

8.664 ± 0.334

8.192 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.119

7.554 ± 0.199

2.91 ± 0.116

3.104 ± 0.145

3.691 ± 0.168

7.457 ± 0.238

6.354 ± 0.274

5.516 ± 0.161

0.913 ± 0.072

4.463 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski