Amino acid dipepetide frequency for Mitosporidium daphniae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.831AlaAla: 5.831 ± 0.126	1.246AlaCys: 1.246 ± 0.035	3.236AlaAsp: 3.236 ± 0.054	3.714AlaGlu: 3.714 ± 0.068	3.369AlaPhe: 3.369 ± 0.049	3.363AlaGly: 3.363 ± 0.058	1.534AlaHis: 1.534 ± 0.039	5.259AlaIle: 5.259 ± 0.068	4.059AlaLys: 4.059 ± 0.063	8.107AlaLeu: 8.107 ± 0.11	1.628AlaMet: 1.628 ± 0.044	2.96AlaAsn: 2.96 ± 0.049	3.477AlaPro: 3.477 ± 0.073	2.495AlaGln: 2.495 ± 0.059	3.175AlaArg: 3.175 ± 0.064	7.882AlaSer: 7.882 ± 0.101	4.018AlaThr: 4.018 ± 0.068	3.712AlaVal: 3.712 ± 0.063	0.64AlaTrp: 0.64 ± 0.026	1.912AlaTyr: 1.912 ± 0.04	0.006AlaXaa: 0.006 ± 0.002
Cys	1.115CysAla: 1.115 ± 0.032	0.399CysCys: 0.399 ± 0.022	0.888CysAsp: 0.888 ± 0.029	0.847CysGlu: 0.847 ± 0.027	0.88CysPhe: 0.88 ± 0.025	1.059CysGly: 1.059 ± 0.033	0.434CysHis: 0.434 ± 0.021	1.2CysIle: 1.2 ± 0.034	1.035CysLys: 1.035 ± 0.031	1.639CysLeu: 1.639 ± 0.04	0.361CysMet: 0.361 ± 0.016	0.751CysAsn: 0.751 ± 0.028	0.912CysPro: 0.912 ± 0.033	0.599CysGln: 0.599 ± 0.026	0.906CysArg: 0.906 ± 0.032	1.936CysSer: 1.936 ± 0.042	0.933CysThr: 0.933 ± 0.028	0.983CysVal: 0.983 ± 0.027	0.235CysTrp: 0.235 ± 0.012	0.485CysTyr: 0.485 ± 0.021	0.002CysXaa: 0.002 ± 0.001
Asp	4.169AspAla: 4.169 ± 0.057	0.772AspCys: 0.772 ± 0.028	3.091AspAsp: 3.091 ± 0.063	3.591AspGlu: 3.591 ± 0.061	2.506AspPhe: 2.506 ± 0.048	3.072AspGly: 3.072 ± 0.057	1.256AspHis: 1.256 ± 0.033	3.457AspIle: 3.457 ± 0.045	2.366AspLys: 2.366 ± 0.04	5.192AspLeu: 5.192 ± 0.07	0.956AspMet: 0.956 ± 0.032	1.593AspAsn: 1.593 ± 0.038	2.972AspPro: 2.972 ± 0.047	1.823AspGln: 1.823 ± 0.041	2.305AspArg: 2.305 ± 0.045	4.577AspSer: 4.577 ± 0.056	2.382AspThr: 2.382 ± 0.042	2.98AspVal: 2.98 ± 0.044	0.497AspTrp: 0.497 ± 0.022	1.309AspTyr: 1.309 ± 0.036	0.007AspXaa: 0.007 ± 0.002
Glu	4.705GluAla: 4.705 ± 0.081	0.878GluCys: 0.878 ± 0.025	3.581GluAsp: 3.581 ± 0.075	4.597GluGlu: 4.597 ± 0.082	2.342GluPhe: 2.342 ± 0.046	2.714GluGly: 2.714 ± 0.05	1.445GluHis: 1.445 ± 0.036	4.142GluIle: 4.142 ± 0.075	4.547GluLys: 4.547 ± 0.079	5.644GluLeu: 5.644 ± 0.082	1.366GluMet: 1.366 ± 0.032	3.322GluAsn: 3.322 ± 0.057	2.135GluPro: 2.135 ± 0.048	2.111GluGln: 2.111 ± 0.044	3.36GluArg: 3.36 ± 0.076	4.781GluSer: 4.781 ± 0.068	2.887GluThr: 2.887 ± 0.049	2.579GluVal: 2.579 ± 0.047	0.591GluTrp: 0.591 ± 0.025	1.78GluTyr: 1.78 ± 0.04	0.006GluXaa: 0.006 ± 0.002
Phe	2.971PheAla: 2.971 ± 0.051	1.075PheCys: 1.075 ± 0.033	2.698PheAsp: 2.698 ± 0.041	2.967PheGlu: 2.967 ± 0.058	2.776PhePhe: 2.776 ± 0.061	2.915PheGly: 2.915 ± 0.062	1.107PheHis: 1.107 ± 0.03	3.185PheIle: 3.185 ± 0.066	2.62PheLys: 2.62 ± 0.055	5.541PheLeu: 5.541 ± 0.087	0.906PheMet: 0.906 ± 0.03	1.852PheAsn: 1.852 ± 0.042	2.542PhePro: 2.542 ± 0.045	1.627PheGln: 1.627 ± 0.039	1.991PheArg: 1.991 ± 0.04	5.879PheSer: 5.879 ± 0.079	2.139PheThr: 2.139 ± 0.045	2.832PheVal: 2.832 ± 0.047	0.583PheTrp: 0.583 ± 0.023	1.711PheTyr: 1.711 ± 0.036	0.006PheXaa: 0.006 ± 0.002
Gly	3.702GlyAla: 3.702 ± 0.074	0.922GlyCys: 0.922 ± 0.031	2.75GlyAsp: 2.75 ± 0.048	2.733GlyGlu: 2.733 ± 0.055	2.465GlyPhe: 2.465 ± 0.049	3.385GlyGly: 3.385 ± 0.074	1.487GlyHis: 1.487 ± 0.038	3.895GlyIle: 3.895 ± 0.052	3.019GlyLys: 3.019 ± 0.053	4.843GlyLeu: 4.843 ± 0.079	1.115GlyMet: 1.115 ± 0.034	2.242GlyAsn: 2.242 ± 0.042	2.319GlyPro: 2.319 ± 0.045	1.668GlyGln: 1.668 ± 0.045	2.669GlyArg: 2.669 ± 0.059	5.232GlySer: 5.232 ± 0.081	2.92GlyThr: 2.92 ± 0.053	2.923GlyVal: 2.923 ± 0.052	0.566GlyTrp: 0.566 ± 0.027	1.541GlyTyr: 1.541 ± 0.042	0.002GlyXaa: 0.002 ± 0.001
His	1.65HisAla: 1.65 ± 0.037	0.444HisCys: 0.444 ± 0.02	0.915HisAsp: 0.915 ± 0.024	1.259HisGlu: 1.259 ± 0.031	1.262HisPhe: 1.262 ± 0.034	1.314HisGly: 1.314 ± 0.035	0.854HisHis: 0.854 ± 0.04	1.486HisIle: 1.486 ± 0.035	1.087HisLys: 1.087 ± 0.029	2.704HisLeu: 2.704 ± 0.046	0.52HisMet: 0.52 ± 0.019	0.754HisAsn: 0.754 ± 0.025	1.506HisPro: 1.506 ± 0.036	1.059HisGln: 1.059 ± 0.032	1.276HisArg: 1.276 ± 0.036	2.354HisSer: 2.354 ± 0.043	1.001HisThr: 1.001 ± 0.027	1.313HisVal: 1.313 ± 0.034	0.26HisTrp: 0.26 ± 0.014	0.656HisTyr: 0.656 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	4.505IleAla: 4.505 ± 0.067	1.395IleCys: 1.395 ± 0.033	3.794IleAsp: 3.794 ± 0.057	4.168IleGlu: 4.168 ± 0.059	3.827IlePhe: 3.827 ± 0.077	3.465IleGly: 3.465 ± 0.055	1.503IleHis: 1.503 ± 0.034	3.866IleIle: 3.866 ± 0.07	3.635IleLys: 3.635 ± 0.067	7.328IleLeu: 7.328 ± 0.085	1.104IleMet: 1.104 ± 0.03	2.523IleAsn: 2.523 ± 0.05	3.713IlePro: 3.713 ± 0.061	2.286IleGln: 2.286 ± 0.045	3.097IleArg: 3.097 ± 0.053	7.766IleSer: 7.766 ± 0.1	2.946IleThr: 2.946 ± 0.052	3.67IleVal: 3.67 ± 0.053	0.651IleTrp: 0.651 ± 0.025	2.042IleTyr: 2.042 ± 0.056	0.003IleXaa: 0.003 ± 0.002
Lys	4.201LysAla: 4.201 ± 0.075	0.878LysCys: 0.878 ± 0.028	3.19LysAsp: 3.19 ± 0.052	4.127LysGlu: 4.127 ± 0.072	2.373LysPhe: 2.373 ± 0.049	2.868LysGly: 2.868 ± 0.052	1.205LysHis: 1.205 ± 0.03	4.212LysIle: 4.212 ± 0.075	4.929LysLys: 4.929 ± 0.084	5.393LysLeu: 5.393 ± 0.087	1.41LysMet: 1.41 ± 0.037	2.899LysAsn: 2.899 ± 0.054	2.467LysPro: 2.467 ± 0.046	1.769LysGln: 1.769 ± 0.042	3.406LysArg: 3.406 ± 0.059	5.186LysSer: 5.186 ± 0.079	2.953LysThr: 2.953 ± 0.052	2.891LysVal: 2.891 ± 0.055	0.606LysTrp: 0.606 ± 0.021	1.939LysTyr: 1.939 ± 0.047	0.005LysXaa: 0.005 ± 0.002
Leu	7.711LeuAla: 7.711 ± 0.114	1.939LeuCys: 1.939 ± 0.048	5.327LeuAsp: 5.327 ± 0.069	6.304LeuGlu: 6.304 ± 0.088	5.767LeuPhe: 5.767 ± 0.088	5.225LeuGly: 5.225 ± 0.072	2.428LeuHis: 2.428 ± 0.046	6.259LeuIle: 6.259 ± 0.074	6.387LeuLys: 6.387 ± 0.091	12.432LeuLeu: 12.432 ± 0.148	2.071LeuMet: 2.071 ± 0.041	4.279LeuAsn: 4.279 ± 0.061	5.344LeuPro: 5.344 ± 0.075	3.965LeuGln: 3.965 ± 0.061	4.951LeuArg: 4.951 ± 0.063	11.125LeuSer: 11.125 ± 0.115	4.408LeuThr: 4.408 ± 0.053	5.993LeuVal: 5.993 ± 0.091	1.047LeuTrp: 1.047 ± 0.039	2.845LeuTyr: 2.845 ± 0.057	0.008LeuXaa: 0.008 ± 0.003
Met	1.929MetAla: 1.929 ± 0.043	0.257MetCys: 0.257 ± 0.014	1.213MetAsp: 1.213 ± 0.029	1.394MetGlu: 1.394 ± 0.033	0.774MetPhe: 0.774 ± 0.025	1.077MetGly: 1.077 ± 0.035	0.565MetHis: 0.565 ± 0.025	1.197MetIle: 1.197 ± 0.026	1.249MetLys: 1.249 ± 0.03	2.342MetLeu: 2.342 ± 0.045	0.558MetMet: 0.558 ± 0.023	0.88MetAsn: 0.88 ± 0.025	1.201MetPro: 1.201 ± 0.034	0.836MetGln: 0.836 ± 0.028	0.936MetArg: 0.936 ± 0.03	1.708MetSer: 1.708 ± 0.035	0.956MetThr: 0.956 ± 0.026	1.261MetVal: 1.261 ± 0.033	0.158MetTrp: 0.158 ± 0.011	0.454MetTyr: 0.454 ± 0.02	0.002MetXaa: 0.002 ± 0.001
Asn	3.117AsnAla: 3.117 ± 0.046	0.751AsnCys: 0.751 ± 0.027	2.068AsnAsp: 2.068 ± 0.046	2.437AsnGlu: 2.437 ± 0.052	2.082AsnPhe: 2.082 ± 0.047	2.607AsnGly: 2.607 ± 0.053	0.925AsnHis: 0.925 ± 0.025	3.055AsnIle: 3.055 ± 0.058	1.809AsnLys: 1.809 ± 0.037	4.211AsnLeu: 4.211 ± 0.067	0.973AsnMet: 0.973 ± 0.028	1.571AsnAsn: 1.571 ± 0.047	2.37AsnPro: 2.37 ± 0.055	1.438AsnGln: 1.438 ± 0.036	1.898AsnArg: 1.898 ± 0.041	4.306AsnSer: 4.306 ± 0.074	2.037AsnThr: 2.037 ± 0.043	2.534AsnVal: 2.534 ± 0.052	0.478AsnTrp: 0.478 ± 0.017	1.079AsnTyr: 1.079 ± 0.028	0.001AsnXaa: 0.001 ± 0.001
Pro	3.318ProAla: 3.318 ± 0.061	0.873ProCys: 0.873 ± 0.03	2.213ProAsp: 2.213 ± 0.048	2.91ProGlu: 2.91 ± 0.059	2.772ProPhe: 2.772 ± 0.044	2.438ProGly: 2.438 ± 0.052	1.182ProHis: 1.182 ± 0.035	3.577ProIle: 3.577 ± 0.057	2.935ProLys: 2.935 ± 0.049	5.227ProLeu: 5.227 ± 0.071	1.007ProMet: 1.007 ± 0.03	2.326ProAsn: 2.326 ± 0.045	3.069ProPro: 3.069 ± 0.086	1.943ProGln: 1.943 ± 0.043	1.946ProArg: 1.946 ± 0.045	6.157ProSer: 6.157 ± 0.096	2.826ProThr: 2.826 ± 0.057	2.832ProVal: 2.832 ± 0.051	0.522ProTrp: 0.522 ± 0.02	1.349ProTyr: 1.349 ± 0.038	0.004ProXaa: 0.004 ± 0.002
Gln	2.538GlnAla: 2.538 ± 0.063	0.518GlnCys: 0.518 ± 0.019	1.573GlnAsp: 1.573 ± 0.041	2.269GlnGlu: 2.269 ± 0.06	1.48GlnPhe: 1.48 ± 0.035	1.595GlnGly: 1.595 ± 0.039	0.885GlnHis: 0.885 ± 0.031	2.298GlnIle: 2.298 ± 0.048	2.636GlnLys: 2.636 ± 0.053	3.649GlnLeu: 3.649 ± 0.061	0.926GlnMet: 0.926 ± 0.026	1.761GlnAsn: 1.761 ± 0.033	1.612GlnPro: 1.612 ± 0.049	1.61GlnGln: 1.61 ± 0.053	1.972GlnArg: 1.972 ± 0.048	3.011GlnSer: 3.011 ± 0.05	1.73GlnThr: 1.73 ± 0.04	1.835GlnVal: 1.835 ± 0.033	0.377GlnTrp: 0.377 ± 0.019	0.969GlnTyr: 0.969 ± 0.027	0.002GlnXaa: 0.002 ± 0.001
Arg	3.261ArgAla: 3.261 ± 0.061	0.857ArgCys: 0.857 ± 0.027	2.206ArgAsp: 2.206 ± 0.049	2.73ArgGlu: 2.73 ± 0.061	2.281ArgPhe: 2.281 ± 0.041	2.282ArgGly: 2.282 ± 0.053	1.279ArgHis: 1.279 ± 0.034	3.417ArgIle: 3.417 ± 0.05	3.098ArgLys: 3.098 ± 0.056	5.233ArgLeu: 5.233 ± 0.087	1.109ArgMet: 1.109 ± 0.033	2.014ArgAsn: 2.014 ± 0.041	2.281ArgPro: 2.281 ± 0.053	1.735ArgGln: 1.735 ± 0.042	3.043ArgArg: 3.043 ± 0.065	4.295ArgSer: 4.295 ± 0.074	2.104ArgThr: 2.104 ± 0.046	2.495ArgVal: 2.495 ± 0.05	0.548ArgTrp: 0.548 ± 0.024	1.434ArgTyr: 1.434 ± 0.035	0.002ArgXaa: 0.002 ± 0.001
Ser	6.885SerAla: 6.885 ± 0.088	1.733SerCys: 1.733 ± 0.043	4.662SerAsp: 4.662 ± 0.066	5.386SerGlu: 5.386 ± 0.076	5.584SerPhe: 5.584 ± 0.079	5.2SerGly: 5.2 ± 0.075	2.239SerHis: 2.239 ± 0.038	7.585SerIle: 7.585 ± 0.1	5.981SerLys: 5.981 ± 0.087	10.932SerLeu: 10.932 ± 0.112	2.213SerMet: 2.213 ± 0.038	4.355SerAsn: 4.355 ± 0.073	5.597SerPro: 5.597 ± 0.099	3.518SerGln: 3.518 ± 0.061	4.25SerArg: 4.25 ± 0.064	13.9SerSer: 13.9 ± 0.2	5.787SerThr: 5.787 ± 0.072	5.118SerVal: 5.118 ± 0.062	0.965SerTrp: 0.965 ± 0.027	2.627SerTyr: 2.627 ± 0.053	0.006SerXaa: 0.006 ± 0.002
Thr	3.506ThrAla: 3.506 ± 0.066	0.923ThrCys: 0.923 ± 0.03	2.238ThrAsp: 2.238 ± 0.047	2.596ThrGlu: 2.596 ± 0.049	2.491ThrPhe: 2.491 ± 0.046	2.915ThrGly: 2.915 ± 0.062	1.131ThrHis: 1.131 ± 0.025	3.342ThrIle: 3.342 ± 0.053	2.588ThrLys: 2.588 ± 0.051	5.349ThrLeu: 5.349 ± 0.068	0.957ThrMet: 0.957 ± 0.028	1.926ThrAsn: 1.926 ± 0.045	3.072ThrPro: 3.072 ± 0.06	1.598ThrGln: 1.598 ± 0.038	2.179ThrArg: 2.179 ± 0.044	5.272ThrSer: 5.272 ± 0.078	2.683ThrThr: 2.683 ± 0.066	2.432ThrVal: 2.432 ± 0.053	0.455ThrTrp: 0.455 ± 0.019	1.193ThrTyr: 1.193 ± 0.033	0.006ThrXaa: 0.006 ± 0.002
Val	4.004ValAla: 4.004 ± 0.062	0.976ValCys: 0.976 ± 0.029	3.153ValAsp: 3.153 ± 0.058	3.389ValGlu: 3.389 ± 0.059	2.787ValPhe: 2.787 ± 0.049	2.877ValGly: 2.877 ± 0.053	1.228ValHis: 1.228 ± 0.033	3.415ValIle: 3.415 ± 0.049	2.798ValLys: 2.798 ± 0.05	5.786ValLeu: 5.786 ± 0.074	0.962ValMet: 0.962 ± 0.028	2.115ValAsn: 2.115 ± 0.041	2.96ValPro: 2.96 ± 0.056	1.857ValGln: 1.857 ± 0.039	2.367ValArg: 2.367 ± 0.053	5.41ValSer: 5.41 ± 0.07	2.414ValThr: 2.414 ± 0.042	3.326ValVal: 3.326 ± 0.057	0.476ValTrp: 0.476 ± 0.021	1.442ValTyr: 1.442 ± 0.038	0.005ValXaa: 0.005 ± 0.002
Trp	0.664TrpAla: 0.664 ± 0.026	0.179TrpCys: 0.179 ± 0.012	0.589TrpAsp: 0.589 ± 0.025	0.496TrpGlu: 0.496 ± 0.023	0.377TrpPhe: 0.377 ± 0.018	0.459TrpGly: 0.459 ± 0.022	0.235TrpHis: 0.235 ± 0.017	0.898TrpIle: 0.898 ± 0.029	0.677TrpLys: 0.677 ± 0.025	1.013TrpLeu: 1.013 ± 0.029	0.282TrpMet: 0.282 ± 0.016	0.553TrpAsn: 0.553 ± 0.02	0.396TrpPro: 0.396 ± 0.02	0.305TrpGln: 0.305 ± 0.017	0.597TrpArg: 0.597 ± 0.028	0.849TrpSer: 0.849 ± 0.027	0.555TrpThr: 0.555 ± 0.021	0.567TrpVal: 0.567 ± 0.02	0.133TrpTrp: 0.133 ± 0.011	0.268TrpTyr: 0.268 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.838TyrAla: 1.838 ± 0.034	0.581TyrCys: 0.581 ± 0.027	1.486TyrAsp: 1.486 ± 0.041	1.593TyrGlu: 1.593 ± 0.042	1.725TyrPhe: 1.725 ± 0.04	1.565TyrGly: 1.565 ± 0.042	0.754TyrHis: 0.754 ± 0.027	1.537TyrIle: 1.537 ± 0.035	1.45TyrLys: 1.45 ± 0.042	3.248TyrLeu: 3.248 ± 0.055	0.517TyrMet: 0.517 ± 0.024	1.066TyrAsn: 1.066 ± 0.034	1.475TyrPro: 1.475 ± 0.038	1.003TyrGln: 1.003 ± 0.036	1.377TyrArg: 1.377 ± 0.035	2.884TyrSer: 2.884 ± 0.061	1.17TyrThr: 1.17 ± 0.034	1.466TyrVal: 1.466 ± 0.036	0.3TyrTrp: 0.3 ± 0.015	1.007TyrTyr: 1.007 ± 0.039	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.006XaaAla: 0.006 ± 0.002	0.001XaaCys: 0.001 ± 0.001	0.003XaaAsp: 0.003 ± 0.002	0.006XaaGlu: 0.006 ± 0.002	0.003XaaPhe: 0.003 ± 0.002	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.005XaaIle: 0.005 ± 0.002	0.004XaaLys: 0.004 ± 0.002	0.002XaaLeu: 0.002 ± 0.001	0.005XaaMet: 0.005 ± 0.002	0.003XaaAsn: 0.003 ± 0.002	0.006XaaPro: 0.006 ± 0.002	0.004XaaGln: 0.004 ± 0.002	0.003XaaArg: 0.003 ± 0.002	0.009XaaSer: 0.009 ± 0.003	0.003XaaThr: 0.003 ± 0.002	0.004XaaVal: 0.004 ± 0.002	0.002XaaTrp: 0.002 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 3256 proteins (1247860 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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