Paenibacillus phage HB10c2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fernvirus; Paenibacillus virus Hb10c2

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5A7A2|A0A0B5A7A2_9CAUD Uncharacterized protein OS=Paenibacillus phage HB10c2 OX=1589749 GN=HB_00009 PE=4 SV=1
MM1 pKa = 7.36SADD4 pKa = 4.14DD5 pKa = 4.68SSSADD10 pKa = 2.87IFIYY14 pKa = 10.75GDD16 pKa = 2.91IVTYY20 pKa = 10.38QWDD23 pKa = 4.04EE24 pKa = 4.05VDD26 pKa = 3.54TSATSFKK33 pKa = 10.87EE34 pKa = 3.89DD35 pKa = 3.83LDD37 pKa = 4.09RR38 pKa = 11.84LGDD41 pKa = 3.68VSNLNLYY48 pKa = 9.79INSPGGSVFEE58 pKa = 5.41GIAIHH63 pKa = 6.78NMLKK67 pKa = 9.77RR68 pKa = 11.84HH69 pKa = 5.04KK70 pKa = 10.23AKK72 pKa = 10.86VNVYY76 pKa = 9.71VDD78 pKa = 3.86ALAASIASVIAMAGDD93 pKa = 4.59TIYY96 pKa = 10.34MPKK99 pKa = 10.65NSMLMIHH106 pKa = 6.56HH107 pKa = 7.81PWTFAWGNASEE118 pKa = 4.14MRR120 pKa = 11.84KK121 pKa = 9.26IADD124 pKa = 3.6DD125 pKa = 3.93LDD127 pKa = 5.42RR128 pKa = 11.84IGNSSKK134 pKa = 8.49QTYY137 pKa = 7.65LQKK140 pKa = 11.05AGDD143 pKa = 3.79KK144 pKa = 10.18LTDD147 pKa = 3.35EE148 pKa = 5.27KK149 pKa = 10.96LQEE152 pKa = 4.09MLDD155 pKa = 3.63AEE157 pKa = 4.64TWLSADD163 pKa = 3.4EE164 pKa = 4.22AFAYY168 pKa = 8.99GLCDD172 pKa = 3.38VVQDD176 pKa = 4.28ANQIAASINDD186 pKa = 3.97EE187 pKa = 3.75IMNRR191 pKa = 11.84YY192 pKa = 9.73KK193 pKa = 10.56NVPKK197 pKa = 10.56QLVSQQKK204 pKa = 8.14TPIFDD209 pKa = 3.38QEE211 pKa = 4.12KK212 pKa = 10.3EE213 pKa = 4.03KK214 pKa = 10.79RR215 pKa = 11.84QQIADD220 pKa = 3.8DD221 pKa = 4.15AKK223 pKa = 11.45VNAAYY228 pKa = 9.88INTILGGIFEE238 pKa = 4.6

Molecular weight: 26.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5A7A7|A0A0B5A7A7_9CAUD Membrane protein P6 OS=Paenibacillus phage HB10c2 OX=1589749 GN=HB_00014 PE=4 SV=1
MM1 pKa = 7.82ANIQVLGVPEE11 pKa = 4.26TVRR14 pKa = 11.84KK15 pKa = 9.82IGLFEE20 pKa = 4.15MEE22 pKa = 4.45RR23 pKa = 11.84KK24 pKa = 8.73QAAIVLVKK32 pKa = 9.65KK33 pKa = 8.6TATSIQKK40 pKa = 9.43EE41 pKa = 4.65GKK43 pKa = 9.42SLAPSSPAGRR53 pKa = 11.84KK54 pKa = 8.81KK55 pKa = 10.99SKK57 pKa = 10.48GKK59 pKa = 9.74PGDD62 pKa = 3.55LKK64 pKa = 10.89RR65 pKa = 11.84SIRR68 pKa = 11.84PKK70 pKa = 10.24YY71 pKa = 9.56MEE73 pKa = 5.22GGLSATVVPRR83 pKa = 11.84KK84 pKa = 9.51PKK86 pKa = 9.03GAHH89 pKa = 5.02RR90 pKa = 11.84HH91 pKa = 4.01LVEE94 pKa = 3.84YY95 pKa = 7.99GTRR98 pKa = 11.84QRR100 pKa = 11.84RR101 pKa = 11.84NKK103 pKa = 10.06KK104 pKa = 8.57GANRR108 pKa = 11.84GKK110 pKa = 9.41MPKK113 pKa = 9.78KK114 pKa = 9.07PFMSIAEE121 pKa = 3.97RR122 pKa = 11.84HH123 pKa = 5.43AEE125 pKa = 3.58GRR127 pKa = 11.84YY128 pKa = 9.23NKK130 pKa = 9.55EE131 pKa = 3.54LEE133 pKa = 4.5RR134 pKa = 11.84IFSRR138 pKa = 11.84DD139 pKa = 2.98EE140 pKa = 4.15TII142 pKa = 4.43

Molecular weight: 15.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10799

53

878

192.8

21.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.602 ± 0.511

0.907 ± 0.132

5.686 ± 0.273

8.056 ± 0.381

3.787 ± 0.205

6.056 ± 0.383

1.806 ± 0.191

7.195 ± 0.31

8.723 ± 0.389

8.167 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.963 ± 0.208

4.565 ± 0.216

3.389 ± 0.237

4.204 ± 0.238

4.815 ± 0.383

6.315 ± 0.289

5.528 ± 0.241

6.139 ± 0.243

1.454 ± 0.2

3.639 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski