Amino acid dipepetide frequency for Streptomyces phage Diane

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.774AlaAla: 11.774 ± 1.514
0.515AlaCys: 0.515 ± 0.193
8.364AlaAsp: 8.364 ± 0.716
9.072AlaGlu: 9.072 ± 0.796
3.024AlaPhe: 3.024 ± 0.491
8.3AlaGly: 8.3 ± 0.749
2.123AlaHis: 2.123 ± 0.442
5.083AlaIle: 5.083 ± 0.632
5.79AlaLys: 5.79 ± 0.706
11.066AlaLeu: 11.066 ± 1.15
2.445AlaMet: 2.445 ± 0.392
2.96AlaAsn: 2.96 ± 0.511
4.182AlaPro: 4.182 ± 0.508
3.41AlaGln: 3.41 ± 0.595
6.112AlaArg: 6.112 ± 0.542
6.884AlaSer: 6.884 ± 0.616
6.176AlaThr: 6.176 ± 0.591
7.399AlaVal: 7.399 ± 0.737
2.123AlaTrp: 2.123 ± 0.289
3.088AlaTyr: 3.088 ± 0.587
0.0AlaXaa: 0.0 ± 0.0
Cys
1.094CysAla: 1.094 ± 0.305
0.0CysCys: 0.0 ± 0.0
0.708CysAsp: 0.708 ± 0.227
0.322CysGlu: 0.322 ± 0.16
0.193CysPhe: 0.193 ± 0.108
0.772CysGly: 0.772 ± 0.182
0.386CysHis: 0.386 ± 0.132
0.193CysIle: 0.193 ± 0.109
0.708CysLys: 0.708 ± 0.242
0.579CysLeu: 0.579 ± 0.191
0.064CysMet: 0.064 ± 0.06
0.129CysAsn: 0.129 ± 0.085
0.643CysPro: 0.643 ± 0.233
0.193CysGln: 0.193 ± 0.103
0.45CysArg: 0.45 ± 0.182
0.45CysSer: 0.45 ± 0.177
0.515CysThr: 0.515 ± 0.211
0.257CysVal: 0.257 ± 0.132
0.386CysTrp: 0.386 ± 0.181
0.322CysTyr: 0.322 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
6.691AspAla: 6.691 ± 0.695
0.708AspCys: 0.708 ± 0.193
5.018AspAsp: 5.018 ± 0.664
5.79AspGlu: 5.79 ± 0.914
1.673AspPhe: 1.673 ± 0.282
6.948AspGly: 6.948 ± 0.659
1.222AspHis: 1.222 ± 0.313
2.316AspIle: 2.316 ± 0.355
2.509AspLys: 2.509 ± 0.458
5.276AspLeu: 5.276 ± 0.567
1.608AspMet: 1.608 ± 0.257
1.094AspAsn: 1.094 ± 0.221
4.182AspPro: 4.182 ± 0.617
2.187AspGln: 2.187 ± 0.379
3.217AspArg: 3.217 ± 0.427
4.053AspSer: 4.053 ± 0.552
3.346AspThr: 3.346 ± 0.41
4.311AspVal: 4.311 ± 0.59
1.673AspTrp: 1.673 ± 0.328
1.48AspTyr: 1.48 ± 0.25
0.0AspXaa: 0.0 ± 0.0
Glu
8.493GluAla: 8.493 ± 0.95
0.772GluCys: 0.772 ± 0.252
5.147GluAsp: 5.147 ± 0.678
5.469GluGlu: 5.469 ± 0.935
1.994GluPhe: 1.994 ± 0.314
6.369GluGly: 6.369 ± 0.688
1.608GluHis: 1.608 ± 0.417
3.088GluIle: 3.088 ± 0.364
3.153GluLys: 3.153 ± 0.423
8.171GluLeu: 8.171 ± 0.907
1.029GluMet: 1.029 ± 0.22
1.158GluAsn: 1.158 ± 0.237
2.702GluPro: 2.702 ± 0.571
3.667GluGln: 3.667 ± 0.462
4.439GluArg: 4.439 ± 0.644
3.603GluSer: 3.603 ± 0.471
3.989GluThr: 3.989 ± 0.701
4.825GluVal: 4.825 ± 0.447
1.48GluTrp: 1.48 ± 0.345
2.574GluTyr: 2.574 ± 0.509
0.0GluXaa: 0.0 ± 0.0
Phe
3.86PheAla: 3.86 ± 0.619
0.129PheCys: 0.129 ± 0.085
2.059PheAsp: 2.059 ± 0.368
2.316PheGlu: 2.316 ± 0.374
0.579PhePhe: 0.579 ± 0.244
2.895PheGly: 2.895 ± 0.402
0.772PheHis: 0.772 ± 0.285
1.415PheIle: 1.415 ± 0.317
0.901PheLys: 0.901 ± 0.247
1.737PheLeu: 1.737 ± 0.371
0.708PheMet: 0.708 ± 0.254
1.222PheAsn: 1.222 ± 0.253
0.901PhePro: 0.901 ± 0.25
0.965PheGln: 0.965 ± 0.222
1.93PheArg: 1.93 ± 0.368
1.351PheSer: 1.351 ± 0.278
2.702PheThr: 2.702 ± 0.436
1.801PheVal: 1.801 ± 0.371
0.386PheTrp: 0.386 ± 0.118
0.965PheTyr: 0.965 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
8.621GlyAla: 8.621 ± 0.937
0.386GlyCys: 0.386 ± 0.153
4.439GlyAsp: 4.439 ± 0.692
5.597GlyGlu: 5.597 ± 0.597
2.767GlyPhe: 2.767 ± 0.435
6.112GlyGly: 6.112 ± 0.857
2.123GlyHis: 2.123 ± 0.444
3.796GlyIle: 3.796 ± 0.771
5.018GlyLys: 5.018 ± 0.616
6.82GlyLeu: 6.82 ± 0.817
0.901GlyMet: 0.901 ± 0.217
2.702GlyAsn: 2.702 ± 0.351
3.925GlyPro: 3.925 ± 0.513
2.831GlyGln: 2.831 ± 0.505
5.34GlyArg: 5.34 ± 0.761
5.404GlySer: 5.404 ± 0.974
5.083GlyThr: 5.083 ± 0.678
5.983GlyVal: 5.983 ± 0.62
1.994GlyTrp: 1.994 ± 0.469
2.638GlyTyr: 2.638 ± 0.345
0.0GlyXaa: 0.0 ± 0.0
His
1.608HisAla: 1.608 ± 0.277
0.193HisCys: 0.193 ± 0.095
1.029HisAsp: 1.029 ± 0.25
1.415HisGlu: 1.415 ± 0.339
1.029HisPhe: 1.029 ± 0.271
1.608HisGly: 1.608 ± 0.275
0.708HisHis: 0.708 ± 0.208
0.45HisIle: 0.45 ± 0.189
0.515HisLys: 0.515 ± 0.188
2.123HisLeu: 2.123 ± 0.386
0.386HisMet: 0.386 ± 0.128
0.45HisAsn: 0.45 ± 0.148
1.029HisPro: 1.029 ± 0.241
0.772HisGln: 0.772 ± 0.258
1.544HisArg: 1.544 ± 0.407
1.351HisSer: 1.351 ± 0.457
1.608HisThr: 1.608 ± 0.289
1.094HisVal: 1.094 ± 0.342
0.643HisTrp: 0.643 ± 0.246
0.579HisTyr: 0.579 ± 0.218
0.0HisXaa: 0.0 ± 0.0
Ile
4.697IleAla: 4.697 ± 0.474
0.386IleCys: 0.386 ± 0.155
3.41IleAsp: 3.41 ± 0.494
3.474IleGlu: 3.474 ± 0.44
1.287IlePhe: 1.287 ± 0.287
3.281IleGly: 3.281 ± 0.56
0.965IleHis: 0.965 ± 0.209
1.866IleIle: 1.866 ± 0.461
2.509IleLys: 2.509 ± 0.485
2.895IleLeu: 2.895 ± 0.412
0.45IleMet: 0.45 ± 0.173
1.415IleAsn: 1.415 ± 0.27
2.187IlePro: 2.187 ± 0.4
1.608IleGln: 1.608 ± 0.316
2.895IleArg: 2.895 ± 0.362
2.574IleSer: 2.574 ± 0.492
2.509IleThr: 2.509 ± 0.392
3.539IleVal: 3.539 ± 0.473
0.322IleTrp: 0.322 ± 0.123
1.287IleTyr: 1.287 ± 0.273
0.0IleXaa: 0.0 ± 0.0
Lys
4.568LysAla: 4.568 ± 0.915
0.386LysCys: 0.386 ± 0.146
2.445LysAsp: 2.445 ± 0.432
2.767LysGlu: 2.767 ± 0.433
1.094LysPhe: 1.094 ± 0.221
5.276LysGly: 5.276 ± 0.596
0.643LysHis: 0.643 ± 0.217
2.38LysIle: 2.38 ± 0.498
3.667LysLys: 3.667 ± 0.522
4.697LysLeu: 4.697 ± 0.536
0.708LysMet: 0.708 ± 0.181
1.094LysAsn: 1.094 ± 0.282
3.024LysPro: 3.024 ± 0.599
1.866LysGln: 1.866 ± 0.307
2.96LysArg: 2.96 ± 0.533
2.38LysSer: 2.38 ± 0.578
2.445LysThr: 2.445 ± 0.333
2.895LysVal: 2.895 ± 0.59
0.45LysTrp: 0.45 ± 0.173
1.48LysTyr: 1.48 ± 0.412
0.0LysXaa: 0.0 ± 0.0
Leu
11.002LeuAla: 11.002 ± 0.916
0.772LeuCys: 0.772 ± 0.277
5.469LeuAsp: 5.469 ± 0.654
5.597LeuGlu: 5.597 ± 0.733
1.93LeuPhe: 1.93 ± 0.525
7.528LeuGly: 7.528 ± 1.075
1.415LeuHis: 1.415 ± 0.35
3.732LeuIle: 3.732 ± 0.407
3.474LeuLys: 3.474 ± 0.756
7.077LeuLeu: 7.077 ± 0.779
1.93LeuMet: 1.93 ± 0.393
2.895LeuAsn: 2.895 ± 0.405
5.018LeuPro: 5.018 ± 0.502
2.509LeuGln: 2.509 ± 0.392
6.498LeuArg: 6.498 ± 0.941
5.662LeuSer: 5.662 ± 0.647
5.404LeuThr: 5.404 ± 0.55
5.211LeuVal: 5.211 ± 0.57
0.965LeuTrp: 0.965 ± 0.247
1.994LeuTyr: 1.994 ± 0.366
0.0LeuXaa: 0.0 ± 0.0
Met
3.539MetAla: 3.539 ± 0.498
0.257MetCys: 0.257 ± 0.121
1.287MetAsp: 1.287 ± 0.274
1.287MetGlu: 1.287 ± 0.287
0.579MetPhe: 0.579 ± 0.195
0.965MetGly: 0.965 ± 0.259
0.515MetHis: 0.515 ± 0.182
0.708MetIle: 0.708 ± 0.185
0.836MetLys: 0.836 ± 0.308
1.866MetLeu: 1.866 ± 0.425
0.193MetMet: 0.193 ± 0.11
0.643MetAsn: 0.643 ± 0.196
0.836MetPro: 0.836 ± 0.189
0.643MetGln: 0.643 ± 0.148
1.415MetArg: 1.415 ± 0.319
1.608MetSer: 1.608 ± 0.329
1.544MetThr: 1.544 ± 0.344
1.222MetVal: 1.222 ± 0.27
0.322MetTrp: 0.322 ± 0.137
0.45MetTyr: 0.45 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
2.638AsnAla: 2.638 ± 0.411
0.45AsnCys: 0.45 ± 0.177
1.287AsnAsp: 1.287 ± 0.282
2.123AsnGlu: 2.123 ± 0.398
0.708AsnPhe: 0.708 ± 0.219
3.217AsnGly: 3.217 ± 0.521
0.515AsnHis: 0.515 ± 0.186
1.48AsnIle: 1.48 ± 0.261
0.965AsnLys: 0.965 ± 0.176
2.38AsnLeu: 2.38 ± 0.355
0.45AsnMet: 0.45 ± 0.163
0.708AsnAsn: 0.708 ± 0.209
1.094AsnPro: 1.094 ± 0.276
1.029AsnGln: 1.029 ± 0.207
1.544AsnArg: 1.544 ± 0.253
1.287AsnSer: 1.287 ± 0.283
1.737AsnThr: 1.737 ± 0.307
1.801AsnVal: 1.801 ± 0.297
0.45AsnTrp: 0.45 ± 0.165
1.029AsnTyr: 1.029 ± 0.27
0.0AsnXaa: 0.0 ± 0.0
Pro
5.083ProAla: 5.083 ± 0.614
0.579ProCys: 0.579 ± 0.191
3.217ProAsp: 3.217 ± 0.366
4.311ProGlu: 4.311 ± 0.625
1.029ProPhe: 1.029 ± 0.231
3.474ProGly: 3.474 ± 0.454
0.45ProHis: 0.45 ± 0.16
2.252ProIle: 2.252 ± 0.395
2.895ProLys: 2.895 ± 0.535
3.088ProLeu: 3.088 ± 0.527
1.158ProMet: 1.158 ± 0.256
1.158ProAsn: 1.158 ± 0.235
1.994ProPro: 1.994 ± 0.346
1.673ProGln: 1.673 ± 0.286
3.217ProArg: 3.217 ± 0.497
3.217ProSer: 3.217 ± 0.65
2.831ProThr: 2.831 ± 0.428
3.86ProVal: 3.86 ± 0.652
0.901ProTrp: 0.901 ± 0.258
1.222ProTyr: 1.222 ± 0.286
0.0ProXaa: 0.0 ± 0.0
Gln
4.504GlnAla: 4.504 ± 0.489
0.257GlnCys: 0.257 ± 0.129
1.48GlnAsp: 1.48 ± 0.257
2.059GlnGlu: 2.059 ± 0.4
1.287GlnPhe: 1.287 ± 0.275
1.93GlnGly: 1.93 ± 0.305
0.322GlnHis: 0.322 ± 0.134
2.187GlnIle: 2.187 ± 0.289
1.608GlnLys: 1.608 ± 0.345
3.153GlnLeu: 3.153 ± 0.805
1.48GlnMet: 1.48 ± 0.302
0.836GlnAsn: 0.836 ± 0.217
1.544GlnPro: 1.544 ± 0.35
1.094GlnGln: 1.094 ± 0.257
2.702GlnArg: 2.702 ± 0.421
2.252GlnSer: 2.252 ± 0.351
1.48GlnThr: 1.48 ± 0.352
3.217GlnVal: 3.217 ± 0.527
0.708GlnTrp: 0.708 ± 0.216
0.772GlnTyr: 0.772 ± 0.189
0.0GlnXaa: 0.0 ± 0.0
Arg
5.855ArgAla: 5.855 ± 0.767
0.643ArgCys: 0.643 ± 0.197
3.86ArgAsp: 3.86 ± 0.457
5.469ArgGlu: 5.469 ± 0.741
2.445ArgPhe: 2.445 ± 0.372
4.568ArgGly: 4.568 ± 0.639
1.158ArgHis: 1.158 ± 0.281
3.153ArgIle: 3.153 ± 0.487
3.217ArgLys: 3.217 ± 0.498
5.34ArgLeu: 5.34 ± 0.669
1.737ArgMet: 1.737 ± 0.411
2.059ArgAsn: 2.059 ± 0.366
3.088ArgPro: 3.088 ± 0.482
2.445ArgGln: 2.445 ± 0.477
4.825ArgArg: 4.825 ± 0.653
3.217ArgSer: 3.217 ± 0.423
3.925ArgThr: 3.925 ± 0.603
4.568ArgVal: 4.568 ± 0.578
0.965ArgTrp: 0.965 ± 0.313
1.866ArgTyr: 1.866 ± 0.336
0.0ArgXaa: 0.0 ± 0.0
Ser
6.048SerAla: 6.048 ± 0.665
0.064SerCys: 0.064 ± 0.065
4.053SerAsp: 4.053 ± 0.503
4.439SerGlu: 4.439 ± 0.485
1.737SerPhe: 1.737 ± 0.327
5.79SerGly: 5.79 ± 0.832
1.351SerHis: 1.351 ± 0.477
2.574SerIle: 2.574 ± 0.415
2.638SerLys: 2.638 ± 0.318
5.147SerLeu: 5.147 ± 0.533
1.222SerMet: 1.222 ± 0.252
1.673SerAsn: 1.673 ± 0.36
2.059SerPro: 2.059 ± 0.291
2.509SerGln: 2.509 ± 0.373
3.989SerArg: 3.989 ± 0.599
4.375SerSer: 4.375 ± 0.939
4.439SerThr: 4.439 ± 0.744
4.118SerVal: 4.118 ± 0.553
1.287SerTrp: 1.287 ± 0.275
2.059SerTyr: 2.059 ± 0.394
0.0SerXaa: 0.0 ± 0.0
Thr
7.013ThrAla: 7.013 ± 0.857
0.643ThrCys: 0.643 ± 0.235
3.539ThrAsp: 3.539 ± 0.413
3.925ThrGlu: 3.925 ± 0.556
2.702ThrPhe: 2.702 ± 0.383
4.632ThrGly: 4.632 ± 0.707
1.287ThrHis: 1.287 ± 0.303
2.445ThrIle: 2.445 ± 0.448
1.673ThrLys: 1.673 ± 0.319
4.439ThrLeu: 4.439 ± 0.509
1.351ThrMet: 1.351 ± 0.335
1.415ThrAsn: 1.415 ± 0.405
3.86ThrPro: 3.86 ± 0.603
1.673ThrGln: 1.673 ± 0.314
3.41ThrArg: 3.41 ± 0.491
5.147ThrSer: 5.147 ± 0.9
3.667ThrThr: 3.667 ± 0.547
5.662ThrVal: 5.662 ± 0.649
0.836ThrTrp: 0.836 ± 0.25
2.187ThrTyr: 2.187 ± 0.413
0.0ThrXaa: 0.0 ± 0.0
Val
7.399ValAla: 7.399 ± 0.52
0.579ValCys: 0.579 ± 0.181
4.632ValAsp: 4.632 ± 0.455
5.083ValGlu: 5.083 ± 0.647
2.059ValPhe: 2.059 ± 0.288
4.375ValGly: 4.375 ± 0.586
1.544ValHis: 1.544 ± 0.322
3.217ValIle: 3.217 ± 0.414
3.474ValLys: 3.474 ± 0.531
6.176ValLeu: 6.176 ± 0.795
1.608ValMet: 1.608 ± 0.296
2.059ValAsn: 2.059 ± 0.311
3.603ValPro: 3.603 ± 0.523
2.767ValGln: 2.767 ± 0.417
4.761ValArg: 4.761 ± 0.565
3.732ValSer: 3.732 ± 0.542
4.761ValThr: 4.761 ± 0.573
4.761ValVal: 4.761 ± 0.656
1.287ValTrp: 1.287 ± 0.301
1.48ValTyr: 1.48 ± 0.347
0.0ValXaa: 0.0 ± 0.0
Trp
2.445TrpAla: 2.445 ± 0.424
0.257TrpCys: 0.257 ± 0.125
1.415TrpAsp: 1.415 ± 0.299
1.094TrpGlu: 1.094 ± 0.236
0.515TrpPhe: 0.515 ± 0.189
1.48TrpGly: 1.48 ± 0.374
0.515TrpHis: 0.515 ± 0.227
0.193TrpIle: 0.193 ± 0.097
0.901TrpLys: 0.901 ± 0.248
1.737TrpLeu: 1.737 ± 0.419
0.643TrpMet: 0.643 ± 0.195
0.386TrpAsn: 0.386 ± 0.158
0.643TrpPro: 0.643 ± 0.199
0.257TrpGln: 0.257 ± 0.178
1.029TrpArg: 1.029 ± 0.212
1.415TrpSer: 1.415 ± 0.32
1.48TrpThr: 1.48 ± 0.242
0.965TrpVal: 0.965 ± 0.306
0.193TrpTrp: 0.193 ± 0.102
0.257TrpTyr: 0.257 ± 0.119
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.96TyrAla: 2.96 ± 0.443
0.386TyrCys: 0.386 ± 0.164
2.445TyrAsp: 2.445 ± 0.437
2.123TyrGlu: 2.123 ± 0.416
0.965TyrPhe: 0.965 ± 0.23
3.024TyrGly: 3.024 ± 0.498
0.515TyrHis: 0.515 ± 0.224
1.029TyrIle: 1.029 ± 0.264
0.772TyrLys: 0.772 ± 0.244
2.316TyrLeu: 2.316 ± 0.403
0.579TyrMet: 0.579 ± 0.202
0.836TyrAsn: 0.836 ± 0.216
1.094TyrPro: 1.094 ± 0.253
0.772TyrGln: 0.772 ± 0.209
2.123TyrArg: 2.123 ± 0.361
1.608TyrSer: 1.608 ± 0.365
1.866TyrThr: 1.866 ± 0.304
1.866TyrVal: 1.866 ± 0.306
0.45TyrTrp: 0.45 ± 0.23
1.158TyrTyr: 1.158 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (15544 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski