Eidolon helvum papillomavirus 1
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4TH86|S4TH86_9PAPI Protein E7 OS=Eidolon helvum papillomavirus 1 OX=1163701 GN=E7 PE=3 SV=1
MM1 pKa = 7.8 RR2 pKa = 11.84 IEE4 pKa = 4.63 LPSLQEE10 pKa = 4.25 LVSQEE15 pKa = 3.81 NPEE18 pKa = 4.39 AVDD21 pKa = 3.91 LNCYY25 pKa = 10.29 EE26 pKa = 4.24 IMSSDD31 pKa = 3.56 EE32 pKa = 4.26 EE33 pKa = 4.37 EE34 pKa = 4.28 VDD36 pKa = 4.15 EE37 pKa = 4.65 EE38 pKa = 4.45 LPLYY42 pKa = 10.51 SVAVHH47 pKa = 6.58 CGSCRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 VRR56 pKa = 11.84 LFCAASRR63 pKa = 11.84 EE64 pKa = 4.17 ALHH67 pKa = 7.04 LLEE70 pKa = 5.54 CLLFEE75 pKa = 5.53 DD76 pKa = 5.64 LLAFLCPGCALAGEE90 pKa = 4.86 DD91 pKa = 4.44 GGG93 pKa = 4.68
Molecular weight: 10.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.011
IPC2_protein 4.253
IPC_protein 4.126
Toseland 3.973
ProMoST 4.228
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.923
Rodwell 3.973
Grimsley 3.897
Solomon 4.05
Lehninger 4.012
Nozaki 4.177
DTASelect 4.266
Thurlkill 3.986
EMBOSS 3.935
Sillero 4.228
Patrickios 0.655
IPC_peptide 4.062
IPC2_peptide 4.228
IPC2.peptide.svr19 4.142
Protein with the highest isoelectric point:
>tr|S4TH17|S4TH17_9PAPI Protein E6 OS=Eidolon helvum papillomavirus 1 OX=1163701 GN=E6 PE=3 SV=1
MM1 pKa = 6.96 EE2 pKa = 4.5 TEE4 pKa = 4.1 KK5 pKa = 10.83 LAKK8 pKa = 9.83 RR9 pKa = 11.84 LSSLEE14 pKa = 3.95 EE15 pKa = 4.21 EE16 pKa = 4.45 ILRR19 pKa = 11.84 HH20 pKa = 4.99 YY21 pKa = 10.71 EE22 pKa = 3.83 KK23 pKa = 10.58 DD24 pKa = 3.45 SKK26 pKa = 11.01 RR27 pKa = 11.84 LEE29 pKa = 3.96 DD30 pKa = 5.64 QIRR33 pKa = 11.84 FWNLMRR39 pKa = 11.84 RR40 pKa = 11.84 EE41 pKa = 3.89 QALMHH46 pKa = 6.85 FIRR49 pKa = 11.84 KK50 pKa = 8.93 QGVHH54 pKa = 5.5 RR55 pKa = 11.84 VGLRR59 pKa = 11.84 PIPSLMASEE68 pKa = 5.32 AEE70 pKa = 3.88 AKK72 pKa = 10.17 KK73 pKa = 10.28 AIEE76 pKa = 4.11 MEE78 pKa = 4.37 VVLTSLSKK86 pKa = 10.67 SQFAFEE92 pKa = 4.25 SWGLRR97 pKa = 11.84 DD98 pKa = 4.12 TSWEE102 pKa = 4.11 LYY104 pKa = 9.42 KK105 pKa = 10.86 APPEE109 pKa = 4.12 NTLKK113 pKa = 10.83 KK114 pKa = 10.4 YY115 pKa = 9.02 GQQVEE120 pKa = 4.45 LTYY123 pKa = 11.42 DD124 pKa = 3.56 NDD126 pKa = 3.88 PDD128 pKa = 3.68 NRR130 pKa = 11.84 SAEE133 pKa = 4.32 TLWGGLYY140 pKa = 10.09 IMYY143 pKa = 10.51 EE144 pKa = 3.74 NDD146 pKa = 2.71 DD147 pKa = 3.44 WRR149 pKa = 11.84 RR150 pKa = 11.84 VTSAVDD156 pKa = 3.22 EE157 pKa = 4.37 KK158 pKa = 11.18 GIYY161 pKa = 9.63 YY162 pKa = 10.06 VDD164 pKa = 3.84 PDD166 pKa = 3.89 GLRR169 pKa = 11.84 IYY171 pKa = 10.25 YY172 pKa = 10.64 VNFEE176 pKa = 4.38 ALADD180 pKa = 4.39 RR181 pKa = 11.84 YY182 pKa = 10.83 GSTGAYY188 pKa = 9.34 KK189 pKa = 10.92 VMAGSDD195 pKa = 4.26 LIAVVGSDD203 pKa = 3.55 TDD205 pKa = 4.45 SEE207 pKa = 4.48 DD208 pKa = 3.23 TTAPDD213 pKa = 3.82 APSIAARR220 pKa = 11.84 RR221 pKa = 11.84 VSSPQTPKK229 pKa = 10.49 PSPKK233 pKa = 8.85 KK234 pKa = 8.57 TPPRR238 pKa = 11.84 KK239 pKa = 7.86 PPRR242 pKa = 11.84 RR243 pKa = 11.84 RR244 pKa = 11.84 RR245 pKa = 11.84 VSTPTRR251 pKa = 11.84 RR252 pKa = 11.84 QRR254 pKa = 11.84 RR255 pKa = 11.84 GEE257 pKa = 3.87 PGSRR261 pKa = 11.84 NRR263 pKa = 11.84 RR264 pKa = 11.84 GARR267 pKa = 11.84 ATAGCTRR274 pKa = 11.84 GEE276 pKa = 4.38 CTPPSPSQVGRR287 pKa = 11.84 RR288 pKa = 11.84 HH289 pKa = 5.85 QSTPPKK295 pKa = 10.28 NLGRR299 pKa = 11.84 LGRR302 pKa = 11.84 LLAEE306 pKa = 5.11 AYY308 pKa = 8.96 DD309 pKa = 4.18 PPILVCRR316 pKa = 11.84 GAANTLKK323 pKa = 9.98 CLRR326 pKa = 11.84 YY327 pKa = 9.22 RR328 pKa = 11.84 VHH330 pKa = 7.07 LKK332 pKa = 10.49 HH333 pKa = 6.84 SNLYY337 pKa = 9.13 DD338 pKa = 3.75 TVSTTWNWTKK348 pKa = 10.83 GSYY351 pKa = 8.08 PQSRR355 pKa = 11.84 IIYY358 pKa = 8.36 LFNSKK363 pKa = 8.95 HH364 pKa = 4.29 QRR366 pKa = 11.84 EE367 pKa = 4.5 SFLEE371 pKa = 4.18 HH372 pKa = 5.86 VKK374 pKa = 10.88 LPASVTHH381 pKa = 5.22 YY382 pKa = 9.92 TGFLNGII389 pKa = 4.22
Molecular weight: 44.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.908
IPC2_protein 9.136
IPC_protein 9.18
Toseland 9.677
ProMoST 9.487
Dawson 9.955
Bjellqvist 9.692
Wikipedia 10.16
Rodwell 10.189
Grimsley 10.058
Solomon 9.984
Lehninger 9.94
Nozaki 9.721
DTASelect 9.677
Thurlkill 9.794
EMBOSS 10.101
Sillero 9.882
Patrickios 5.219
IPC_peptide 9.984
IPC2_peptide 8.39
IPC2.peptide.svr19 8.104
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2311
93
640
385.2
43.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.928 ± 0.27
1.861 ± 0.691
6.145 ± 0.297
7.14 ± 0.427
4.414 ± 0.591
6.621 ± 0.499
2.077 ± 0.155
4.717 ± 0.768
5.019 ± 0.719
8.135 ± 0.747
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.688 ± 0.297
3.721 ± 0.667
6.318 ± 0.884
4.37 ± 0.518
6.707 ± 0.764
6.404 ± 0.634
7.572 ± 0.864
6.491 ± 0.786
1.255 ± 0.33
3.418 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here