Streptococcus phage Javan237
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A795|A0A4D6A795_9CAUD Putative cI-like repressor OS=Streptococcus phage Javan237 OX=2548061 GN=Javan237_0029 PE=4 SV=1
MM1 pKa = 7.48 SVTKK5 pKa = 9.73 EE6 pKa = 4.04 QIMLTLNLDD15 pKa = 3.56 EE16 pKa = 6.51 SDD18 pKa = 5.06 DD19 pKa = 3.79 LTLIPAYY26 pKa = 8.83 ITTAEE31 pKa = 4.33 TYY33 pKa = 10.11 IKK35 pKa = 10.6 NAVGNADD42 pKa = 3.42 GFFEE46 pKa = 4.25 QEE48 pKa = 4.12 NVKK51 pKa = 10.51 DD52 pKa = 4.18 LYY54 pKa = 10.11 DD55 pKa = 3.53 TAVIALASAYY65 pKa = 7.7 YY66 pKa = 8.85 TYY68 pKa = 10.89 RR69 pKa = 11.84 VALADD74 pKa = 3.59 TMAYY78 pKa = 9.78 PIDD81 pKa = 3.85 LTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 9.92 AVYY98 pKa = 10.63 CEE100 pKa = 4.25 EE101 pKa = 4.33 TSNGG105 pKa = 3.47
Molecular weight: 11.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.874
IPC2_protein 3.897
IPC_protein 3.808
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.935
Patrickios 0.172
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A4D6A4E5|A0A4D6A4E5_9CAUD Major tail protein OS=Streptococcus phage Javan237 OX=2548061 GN=Javan237_0015 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 10.17 RR3 pKa = 11.84 NKK5 pKa = 8.81 TKK7 pKa = 10.32 YY8 pKa = 9.53 PSIFTYY14 pKa = 7.28 EE15 pKa = 4.01 TKK17 pKa = 10.15 KK18 pKa = 10.43 GKK20 pKa = 9.9 RR21 pKa = 11.84 YY22 pKa = 8.17 YY23 pKa = 10.5 VRR25 pKa = 11.84 RR26 pKa = 11.84 KK27 pKa = 8.39 FTLNGKK33 pKa = 6.49 QTEE36 pKa = 4.15 ASASGLKK43 pKa = 9.01 TLAEE47 pKa = 4.14 ARR49 pKa = 11.84 QALAEE54 pKa = 4.15 IEE56 pKa = 4.27 NKK58 pKa = 9.36 IANGDD63 pKa = 3.46 YY64 pKa = 10.76 DD65 pKa = 3.63 PRR67 pKa = 11.84 KK68 pKa = 10.68 NMTVDD73 pKa = 4.89 DD74 pKa = 3.82 YY75 pKa = 11.16 WQIYY79 pKa = 8.42 SEE81 pKa = 4.02 NRR83 pKa = 11.84 IKK85 pKa = 9.86 TGRR88 pKa = 11.84 WAPDD92 pKa = 3.78 TIATKK97 pKa = 9.77 MSWYY101 pKa = 9.83 KK102 pKa = 10.36 YY103 pKa = 9.51 HH104 pKa = 6.28 FHH106 pKa = 6.35 KK107 pKa = 10.95 QFGRR111 pKa = 11.84 TLLKK115 pKa = 10.41 DD116 pKa = 3.57 IKK118 pKa = 8.48 RR119 pKa = 11.84 TKK121 pKa = 9.6 YY122 pKa = 8.42 EE123 pKa = 3.99 AYY125 pKa = 9.88 VSSMLKK131 pKa = 9.97 NYY133 pKa = 10.31 SRR135 pKa = 11.84 TSVIQISALFEE146 pKa = 4.44 SMLTDD151 pKa = 4.23 AVINDD156 pKa = 4.49 YY157 pKa = 10.76 IDD159 pKa = 3.63 KK160 pKa = 10.99 NPILRR165 pKa = 11.84 IYY167 pKa = 9.63 IGKK170 pKa = 9.76 SDD172 pKa = 3.65 IPAKK176 pKa = 10.27 KK177 pKa = 9.74 KK178 pKa = 10.44 RR179 pKa = 11.84 LSLEE183 pKa = 4.32 DD184 pKa = 3.46 FRR186 pKa = 11.84 IWDD189 pKa = 3.36 KK190 pKa = 10.74 CARR193 pKa = 11.84 EE194 pKa = 4.01 ILSSYY199 pKa = 10.11 DD200 pKa = 3.22 YY201 pKa = 11.51 TMVRR205 pKa = 11.84 LTYY208 pKa = 9.91 FGLRR212 pKa = 11.84 RR213 pKa = 11.84 SEE215 pKa = 3.92 VLGVKK220 pKa = 10.42 FGSLSLVDD228 pKa = 3.7 GRR230 pKa = 11.84 FKK232 pKa = 10.84 IFLDD236 pKa = 3.56 EE237 pKa = 4.3 SRR239 pKa = 11.84 TSRR242 pKa = 11.84 RR243 pKa = 11.84 PEE245 pKa = 3.81 GGRR248 pKa = 11.84 MKK250 pKa = 10.4 TKK252 pKa = 9.25 TSEE255 pKa = 3.98 RR256 pKa = 11.84 YY257 pKa = 9.47 VLVDD261 pKa = 3.39 EE262 pKa = 4.84 EE263 pKa = 4.68 TTEE266 pKa = 4.24 LLQKK270 pKa = 11.28 AMILSRR276 pKa = 11.84 QIAKK280 pKa = 7.27 EE281 pKa = 3.93 TNRR284 pKa = 11.84 ILGQGDD290 pKa = 4.81 FIFVDD295 pKa = 3.44 TGMKK299 pKa = 8.79 TKK301 pKa = 9.63 NHH303 pKa = 6.55 KK304 pKa = 10.24 GNPIGYY310 pKa = 8.16 SHH312 pKa = 7.66 IAYY315 pKa = 9.9 AFRR318 pKa = 11.84 KK319 pKa = 10.27 VSDD322 pKa = 3.24 KK323 pKa = 10.96 TNIYY327 pKa = 7.62 VTPHH331 pKa = 4.66 TMRR334 pKa = 11.84 HH335 pKa = 5.08 FFATQGQIAGVPVEE349 pKa = 4.8 HH350 pKa = 6.66 MAAALGHH357 pKa = 5.31 STSYY361 pKa = 7.77 MTQKK365 pKa = 8.95 YY366 pKa = 6.41 THH368 pKa = 6.9 IKK370 pKa = 10.67 DD371 pKa = 3.64 EE372 pKa = 4.38 VASEE376 pKa = 4.27 VTDD379 pKa = 3.47 SFLRR383 pKa = 11.84 AIKK386 pKa = 10.4
Molecular weight: 44.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.121
IPC2_protein 9.297
IPC_protein 9.253
Toseland 9.897
ProMoST 9.633
Dawson 10.131
Bjellqvist 9.794
Wikipedia 10.306
Rodwell 10.54
Grimsley 10.218
Solomon 10.145
Lehninger 10.101
Nozaki 9.882
DTASelect 9.794
Thurlkill 9.97
EMBOSS 10.306
Sillero 10.043
Patrickios 9.677
IPC_peptide 10.145
IPC2_peptide 8.302
IPC2.peptide.svr19 8.304
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
33
0
33
8398
39
1530
254.5
28.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.645 ± 0.503
0.286 ± 0.072
6.335 ± 0.45
6.287 ± 0.556
3.858 ± 0.252
6.859 ± 0.502
1.226 ± 0.139
6.371 ± 0.315
8.216 ± 0.63
7.978 ± 0.358
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.489 ± 0.202
6.097 ± 0.322
2.798 ± 0.199
4.322 ± 0.334
3.12 ± 0.277
7.371 ± 0.39
7.514 ± 0.581
5.799 ± 0.265
1.524 ± 0.151
3.906 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here