Streptococcus phage V22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E8ZD65|E8ZD65_9CAUD Phage protein OS=Streptococcus phage V22 OX=870469 PE=4 SV=1
MM1 pKa = 8.35LMFDD5 pKa = 3.85YY6 pKa = 10.77DD7 pKa = 4.39RR8 pKa = 11.84DD9 pKa = 3.45IMQPPEE15 pKa = 3.98EE16 pKa = 4.61RR17 pKa = 11.84EE18 pKa = 4.03EE19 pKa = 4.62LDD21 pKa = 3.41PADD24 pKa = 4.33WIFSAGQWIYY34 pKa = 11.59VGDD37 pKa = 4.05CC38 pKa = 3.39

Molecular weight: 4.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E8ZD71|E8ZD71_9CAUD Phage protein OS=Streptococcus phage V22 OX=870469 PE=4 SV=1
MM1 pKa = 7.77GKK3 pKa = 8.66NQHH6 pKa = 5.58VVPAKK11 pKa = 10.06NGGWNVKK18 pKa = 9.83GAGNSRR24 pKa = 11.84ATVHH28 pKa = 5.29TTTKK32 pKa = 10.93NEE34 pKa = 3.98AKK36 pKa = 10.52NIARR40 pKa = 11.84QISRR44 pKa = 11.84NQGSEE49 pKa = 4.28LIIHH53 pKa = 6.65GKK55 pKa = 9.66DD56 pKa = 3.08GKK58 pKa = 9.98IQSRR62 pKa = 11.84DD63 pKa = 3.33SHH65 pKa = 6.86GKK67 pKa = 10.26DD68 pKa = 3.06PFPPKK73 pKa = 10.63GG74 pKa = 3.28

Molecular weight: 7.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

10723

38

1468

188.1

21.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.976 ± 0.629

0.653 ± 0.115

6.453 ± 0.299

8.319 ± 0.621

4.122 ± 0.301

6.369 ± 0.659

1.334 ± 0.136

6.416 ± 0.25

8.626 ± 0.418

8.029 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.196

5.372 ± 0.259

2.658 ± 0.211

4.169 ± 0.251

4.691 ± 0.365

5.67 ± 0.335

5.885 ± 0.323

6.481 ± 0.279

1.287 ± 0.143

3.907 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski