Proteome-pI 2.0 - Proteome Isoelectric Point Database

Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Erwinia; Erwinia tasmaniensis

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3618 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|B2VL57|B2VL57_ERWT9 Two-component response regulator OS=Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99) OX=465817 GN=cpxR PE=4 SV=1

MM1 pKa = 7.59SEE3 pKa = 3.96QNNTEE8 pKa = 3.74MSFQIQRR15 pKa = 11.84IYY17 pKa = 10.58TKK19 pKa = 10.12DD20 pKa = 3.08ISFEE24 pKa = 4.19APNAPQVFQKK34 pKa = 10.29EE35 pKa = 4.21WEE37 pKa = 4.31PEE39 pKa = 3.99VKK41 pKa = 10.4LDD43 pKa = 4.58LDD45 pKa = 4.02TASSQLADD53 pKa = 3.58DD54 pKa = 4.2VYY56 pKa = 11.21EE57 pKa = 4.1VVLRR61 pKa = 11.84VTVTATVGEE70 pKa = 4.42DD71 pKa = 3.17SAFLCEE77 pKa = 4.17VQQAGIFSVGGIDD90 pKa = 3.81GTQMAHH96 pKa = 7.07CLGAYY101 pKa = 9.15CPNILFPYY109 pKa = 9.96ARR111 pKa = 11.84EE112 pKa = 4.53CITSLVARR120 pKa = 11.84GTFPQLNLAPVNFDD134 pKa = 3.64ALFMNYY140 pKa = 9.29LQQSEE145 pKa = 4.68GAAQQQDD152 pKa = 3.18AA153 pKa = 4.36

Molecular weight: 16.91 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|B2VCJ3|B2VCJ3_ERWT9 Transcriptional regulator of organic hydroperoxide resistance OS=Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99) OX=465817 GN=ohrR PE=4 SV=1

MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MANKK25 pKa = 9.98NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.41 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3618	0	3618	1146452	24	3351	316.9	35.03

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.736 ± 0.053	1.044 ± 0.013	5.274 ± 0.034	5.428 ± 0.041	3.806 ± 0.027	7.412 ± 0.044	2.284 ± 0.022	5.688 ± 0.038	4.254 ± 0.046	11.013 ± 0.057

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.665 ± 0.018	3.776 ± 0.034	4.399 ± 0.025	4.715 ± 0.04	5.939 ± 0.039	6.377 ± 0.037	5.142 ± 0.031	6.852 ± 0.035	1.429 ± 0.017	2.767 ± 0.028

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski