Amino acid dipepetide frequency for Bacteriophage APSE-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.948AlaAla: 7.948 ± 1.488
0.786AlaCys: 0.786 ± 0.288
4.891AlaAsp: 4.891 ± 0.876
6.463AlaGlu: 6.463 ± 0.953
2.97AlaPhe: 2.97 ± 0.571
5.241AlaGly: 5.241 ± 0.707
2.096AlaHis: 2.096 ± 0.469
4.717AlaIle: 4.717 ± 0.551
6.813AlaLys: 6.813 ± 0.907
10.569AlaLeu: 10.569 ± 0.973
2.97AlaMet: 2.97 ± 0.539
3.843AlaAsn: 3.843 ± 0.767
2.533AlaPro: 2.533 ± 0.506
4.018AlaGln: 4.018 ± 0.658
3.319AlaArg: 3.319 ± 0.537
5.066AlaSer: 5.066 ± 0.71
4.193AlaThr: 4.193 ± 0.491
4.629AlaVal: 4.629 ± 0.721
1.66AlaTrp: 1.66 ± 0.417
2.795AlaTyr: 2.795 ± 0.598
0.0AlaXaa: 0.0 ± 0.0
Cys
0.349CysAla: 0.349 ± 0.162
0.262CysCys: 0.262 ± 0.146
0.699CysAsp: 0.699 ± 0.327
0.087CysGlu: 0.087 ± 0.073
0.961CysPhe: 0.961 ± 0.287
0.699CysGly: 0.699 ± 0.265
0.087CysHis: 0.087 ± 0.08
0.611CysIle: 0.611 ± 0.279
0.786CysLys: 0.786 ± 0.258
1.485CysLeu: 1.485 ± 0.308
0.437CysMet: 0.437 ± 0.216
0.262CysAsn: 0.262 ± 0.139
0.524CysPro: 0.524 ± 0.199
0.524CysGln: 0.524 ± 0.263
0.873CysArg: 0.873 ± 0.27
0.437CysSer: 0.437 ± 0.196
0.349CysThr: 0.349 ± 0.158
0.349CysVal: 0.349 ± 0.145
0.087CysTrp: 0.087 ± 0.094
0.524CysTyr: 0.524 ± 0.211
0.0CysXaa: 0.0 ± 0.0
Asp
4.629AspAla: 4.629 ± 0.679
0.437AspCys: 0.437 ± 0.134
3.406AspAsp: 3.406 ± 0.514
4.367AspGlu: 4.367 ± 0.638
1.747AspPhe: 1.747 ± 0.449
4.367AspGly: 4.367 ± 0.572
0.873AspHis: 0.873 ± 0.404
3.494AspIle: 3.494 ± 0.5
3.756AspLys: 3.756 ± 0.557
4.804AspLeu: 4.804 ± 0.666
1.398AspMet: 1.398 ± 0.418
3.406AspAsn: 3.406 ± 0.544
2.882AspPro: 2.882 ± 0.408
1.398AspGln: 1.398 ± 0.363
1.922AspArg: 1.922 ± 0.384
3.144AspSer: 3.144 ± 0.441
3.144AspThr: 3.144 ± 0.527
3.581AspVal: 3.581 ± 0.49
0.873AspTrp: 0.873 ± 0.233
2.009AspTyr: 2.009 ± 0.445
0.0AspXaa: 0.0 ± 0.0
Glu
6.725GluAla: 6.725 ± 0.894
0.611GluCys: 0.611 ± 0.253
2.358GluAsp: 2.358 ± 0.568
3.406GluGlu: 3.406 ± 0.685
1.747GluPhe: 1.747 ± 0.485
3.494GluGly: 3.494 ± 0.467
1.048GluHis: 1.048 ± 0.32
3.668GluIle: 3.668 ± 0.537
5.066GluLys: 5.066 ± 0.592
5.765GluLeu: 5.765 ± 0.585
1.572GluMet: 1.572 ± 0.45
3.319GluAsn: 3.319 ± 0.515
1.747GluPro: 1.747 ± 0.512
2.97GluGln: 2.97 ± 0.505
3.668GluArg: 3.668 ± 0.6
3.232GluSer: 3.232 ± 0.524
3.668GluThr: 3.668 ± 0.631
3.581GluVal: 3.581 ± 0.651
1.135GluTrp: 1.135 ± 0.348
2.184GluTyr: 2.184 ± 0.407
0.0GluXaa: 0.0 ± 0.0
Phe
2.62PheAla: 2.62 ± 0.543
0.873PheCys: 0.873 ± 0.323
2.97PheAsp: 2.97 ± 0.444
1.66PheGlu: 1.66 ± 0.378
1.398PhePhe: 1.398 ± 0.434
2.795PheGly: 2.795 ± 0.511
0.524PheHis: 0.524 ± 0.256
2.009PheIle: 2.009 ± 0.385
2.358PheLys: 2.358 ± 0.412
1.922PheLeu: 1.922 ± 0.307
0.961PheMet: 0.961 ± 0.307
2.358PheAsn: 2.358 ± 0.509
1.135PhePro: 1.135 ± 0.374
1.135PheGln: 1.135 ± 0.291
1.834PheArg: 1.834 ± 0.41
2.62PheSer: 2.62 ± 0.57
2.446PheThr: 2.446 ± 0.514
1.834PheVal: 1.834 ± 0.339
0.262PheTrp: 0.262 ± 0.136
1.31PheTyr: 1.31 ± 0.313
0.0PheXaa: 0.0 ± 0.0
Gly
4.629GlyAla: 4.629 ± 0.628
0.873GlyCys: 0.873 ± 0.233
4.105GlyAsp: 4.105 ± 0.754
4.105GlyGlu: 4.105 ± 0.742
3.144GlyPhe: 3.144 ± 0.425
4.891GlyGly: 4.891 ± 0.846
1.048GlyHis: 1.048 ± 0.293
5.153GlyIle: 5.153 ± 0.58
5.415GlyLys: 5.415 ± 0.544
5.153GlyLeu: 5.153 ± 0.78
1.922GlyMet: 1.922 ± 0.458
2.533GlyAsn: 2.533 ± 0.404
0.873GlyPro: 0.873 ± 0.257
3.668GlyGln: 3.668 ± 0.665
2.882GlyArg: 2.882 ± 0.468
4.367GlySer: 4.367 ± 0.613
3.668GlyThr: 3.668 ± 0.441
3.668GlyVal: 3.668 ± 0.569
0.873GlyTrp: 0.873 ± 0.27
2.358GlyTyr: 2.358 ± 0.484
0.0GlyXaa: 0.0 ± 0.0
His
2.009HisAla: 2.009 ± 0.316
0.0HisCys: 0.0 ± 0.0
1.485HisAsp: 1.485 ± 0.354
1.223HisGlu: 1.223 ± 0.381
0.786HisPhe: 0.786 ± 0.247
0.786HisGly: 0.786 ± 0.289
0.437HisHis: 0.437 ± 0.19
1.223HisIle: 1.223 ± 0.293
0.873HisLys: 0.873 ± 0.212
1.922HisLeu: 1.922 ± 0.397
0.524HisMet: 0.524 ± 0.196
1.398HisAsn: 1.398 ± 0.38
0.873HisPro: 0.873 ± 0.299
1.485HisGln: 1.485 ± 0.391
1.31HisArg: 1.31 ± 0.337
1.31HisSer: 1.31 ± 0.288
1.31HisThr: 1.31 ± 0.434
1.135HisVal: 1.135 ± 0.252
0.437HisTrp: 0.437 ± 0.192
0.699HisTyr: 0.699 ± 0.246
0.0HisXaa: 0.0 ± 0.0
Ile
5.241IleAla: 5.241 ± 0.739
0.611IleCys: 0.611 ± 0.258
3.668IleAsp: 3.668 ± 0.589
5.939IleGlu: 5.939 ± 0.691
2.533IlePhe: 2.533 ± 0.616
4.367IleGly: 4.367 ± 0.685
0.961IleHis: 0.961 ± 0.306
4.105IleIle: 4.105 ± 0.585
3.581IleLys: 3.581 ± 0.741
3.494IleLeu: 3.494 ± 0.355
0.873IleMet: 0.873 ± 0.228
3.581IleAsn: 3.581 ± 0.444
3.057IlePro: 3.057 ± 0.416
2.708IleGln: 2.708 ± 0.402
2.271IleArg: 2.271 ± 0.424
3.93IleSer: 3.93 ± 0.484
3.144IleThr: 3.144 ± 0.434
3.406IleVal: 3.406 ± 0.526
0.524IleTrp: 0.524 ± 0.186
2.271IleTyr: 2.271 ± 0.41
0.0IleXaa: 0.0 ± 0.0
Lys
6.813LysAla: 6.813 ± 0.641
0.611LysCys: 0.611 ± 0.185
3.319LysAsp: 3.319 ± 0.406
3.232LysGlu: 3.232 ± 0.521
1.398LysPhe: 1.398 ± 0.353
4.105LysGly: 4.105 ± 0.491
1.747LysHis: 1.747 ± 0.334
3.843LysIle: 3.843 ± 0.594
4.891LysLys: 4.891 ± 0.632
5.241LysLeu: 5.241 ± 0.716
2.533LysMet: 2.533 ± 0.524
3.494LysAsn: 3.494 ± 0.411
3.232LysPro: 3.232 ± 0.796
3.232LysGln: 3.232 ± 0.526
4.979LysArg: 4.979 ± 0.848
5.59LysSer: 5.59 ± 0.632
4.105LysThr: 4.105 ± 0.542
2.97LysVal: 2.97 ± 0.579
1.398LysTrp: 1.398 ± 0.389
2.62LysTyr: 2.62 ± 0.453
0.0LysXaa: 0.0 ± 0.0
Leu
8.298LeuAla: 8.298 ± 0.584
1.31LeuCys: 1.31 ± 0.338
3.581LeuAsp: 3.581 ± 0.486
4.367LeuGlu: 4.367 ± 0.607
2.62LeuPhe: 2.62 ± 0.455
5.677LeuGly: 5.677 ± 0.831
1.223LeuHis: 1.223 ± 0.306
5.066LeuIle: 5.066 ± 0.684
6.027LeuLys: 6.027 ± 0.722
8.21LeuLeu: 8.21 ± 1.216
2.533LeuMet: 2.533 ± 0.558
4.717LeuAsn: 4.717 ± 0.648
4.455LeuPro: 4.455 ± 0.896
3.843LeuGln: 3.843 ± 0.578
5.852LeuArg: 5.852 ± 0.878
6.813LeuSer: 6.813 ± 0.696
5.939LeuThr: 5.939 ± 0.544
3.756LeuVal: 3.756 ± 0.581
0.873LeuTrp: 0.873 ± 0.263
2.708LeuTyr: 2.708 ± 0.585
0.0LeuXaa: 0.0 ± 0.0
Met
2.708MetAla: 2.708 ± 0.428
0.262MetCys: 0.262 ± 0.147
1.048MetAsp: 1.048 ± 0.289
1.223MetGlu: 1.223 ± 0.328
0.699MetPhe: 0.699 ± 0.192
1.66MetGly: 1.66 ± 0.36
0.524MetHis: 0.524 ± 0.221
1.485MetIle: 1.485 ± 0.299
2.096MetLys: 2.096 ± 0.478
2.271MetLeu: 2.271 ± 0.395
0.961MetMet: 0.961 ± 0.302
1.223MetAsn: 1.223 ± 0.351
1.048MetPro: 1.048 ± 0.387
1.747MetGln: 1.747 ± 0.42
1.66MetArg: 1.66 ± 0.345
1.572MetSer: 1.572 ± 0.371
1.66MetThr: 1.66 ± 0.506
1.485MetVal: 1.485 ± 0.266
0.262MetTrp: 0.262 ± 0.162
0.786MetTyr: 0.786 ± 0.238
0.0MetXaa: 0.0 ± 0.0
Asn
4.979AsnAla: 4.979 ± 0.856
0.873AsnCys: 0.873 ± 0.363
2.446AsnAsp: 2.446 ± 0.451
2.271AsnGlu: 2.271 ± 0.342
0.961AsnPhe: 0.961 ± 0.347
3.319AsnGly: 3.319 ± 0.738
1.834AsnHis: 1.834 ± 0.354
3.057AsnIle: 3.057 ± 0.362
3.756AsnLys: 3.756 ± 0.59
4.28AsnLeu: 4.28 ± 0.709
1.135AsnMet: 1.135 ± 0.301
2.533AsnAsn: 2.533 ± 0.457
2.184AsnPro: 2.184 ± 0.508
2.708AsnGln: 2.708 ± 0.552
3.668AsnArg: 3.668 ± 0.531
3.93AsnSer: 3.93 ± 0.608
2.882AsnThr: 2.882 ± 0.371
2.271AsnVal: 2.271 ± 0.472
0.961AsnTrp: 0.961 ± 0.31
1.398AsnTyr: 1.398 ± 0.35
0.0AsnXaa: 0.0 ± 0.0
Pro
2.271ProAla: 2.271 ± 0.517
0.175ProCys: 0.175 ± 0.131
2.882ProAsp: 2.882 ± 0.486
2.358ProGlu: 2.358 ± 0.536
1.135ProPhe: 1.135 ± 0.267
2.533ProGly: 2.533 ± 0.391
1.048ProHis: 1.048 ± 0.271
2.358ProIle: 2.358 ± 0.406
2.795ProLys: 2.795 ± 0.51
3.232ProLeu: 3.232 ± 0.5
0.611ProMet: 0.611 ± 0.27
2.184ProAsn: 2.184 ± 0.439
1.31ProPro: 1.31 ± 0.423
2.533ProGln: 2.533 ± 0.406
2.358ProArg: 2.358 ± 0.524
3.144ProSer: 3.144 ± 0.539
2.795ProThr: 2.795 ± 0.506
3.406ProVal: 3.406 ± 0.613
0.349ProTrp: 0.349 ± 0.171
1.398ProTyr: 1.398 ± 0.381
0.0ProXaa: 0.0 ± 0.0
Gln
4.629GlnAla: 4.629 ± 0.695
0.349GlnCys: 0.349 ± 0.162
2.184GlnAsp: 2.184 ± 0.4
2.271GlnGlu: 2.271 ± 0.615
2.184GlnPhe: 2.184 ± 0.44
2.62GlnGly: 2.62 ± 0.616
0.786GlnHis: 0.786 ± 0.27
3.144GlnIle: 3.144 ± 0.507
3.232GlnLys: 3.232 ± 0.501
4.455GlnLeu: 4.455 ± 0.592
1.31GlnMet: 1.31 ± 0.27
2.446GlnAsn: 2.446 ± 0.566
2.184GlnPro: 2.184 ± 0.4
3.494GlnGln: 3.494 ± 0.619
3.319GlnArg: 3.319 ± 0.515
3.232GlnSer: 3.232 ± 0.597
2.358GlnThr: 2.358 ± 0.296
2.271GlnVal: 2.271 ± 0.462
0.611GlnTrp: 0.611 ± 0.198
1.31GlnTyr: 1.31 ± 0.547
0.0GlnXaa: 0.0 ± 0.0
Arg
4.542ArgAla: 4.542 ± 0.559
0.262ArgCys: 0.262 ± 0.15
2.446ArgAsp: 2.446 ± 0.499
3.406ArgGlu: 3.406 ± 0.639
2.795ArgPhe: 2.795 ± 0.432
2.62ArgGly: 2.62 ± 0.47
1.223ArgHis: 1.223 ± 0.295
4.28ArgIle: 4.28 ± 0.531
3.232ArgLys: 3.232 ± 0.519
5.415ArgLeu: 5.415 ± 0.726
1.135ArgMet: 1.135 ± 0.278
3.668ArgAsn: 3.668 ± 0.484
2.533ArgPro: 2.533 ± 0.471
2.358ArgGln: 2.358 ± 0.387
3.756ArgArg: 3.756 ± 0.527
2.446ArgSer: 2.446 ± 0.471
2.009ArgThr: 2.009 ± 0.34
2.795ArgVal: 2.795 ± 0.427
1.572ArgTrp: 1.572 ± 0.379
1.747ArgTyr: 1.747 ± 0.41
0.0ArgXaa: 0.0 ± 0.0
Ser
5.241SerAla: 5.241 ± 0.633
0.699SerCys: 0.699 ± 0.329
3.581SerAsp: 3.581 ± 0.377
3.93SerGlu: 3.93 ± 0.573
2.795SerPhe: 2.795 ± 0.447
4.455SerGly: 4.455 ± 0.584
1.922SerHis: 1.922 ± 0.496
4.193SerIle: 4.193 ± 0.628
3.93SerLys: 3.93 ± 0.634
5.153SerLeu: 5.153 ± 0.773
1.922SerMet: 1.922 ± 0.367
3.144SerAsn: 3.144 ± 0.447
3.668SerPro: 3.668 ± 0.481
3.144SerGln: 3.144 ± 0.499
2.97SerArg: 2.97 ± 0.53
4.455SerSer: 4.455 ± 0.609
3.581SerThr: 3.581 ± 0.696
4.105SerVal: 4.105 ± 0.389
1.048SerTrp: 1.048 ± 0.28
1.485SerTyr: 1.485 ± 0.397
0.0SerXaa: 0.0 ± 0.0
Thr
5.765ThrAla: 5.765 ± 0.841
0.175ThrCys: 0.175 ± 0.118
3.057ThrAsp: 3.057 ± 0.462
3.581ThrGlu: 3.581 ± 0.468
1.834ThrPhe: 1.834 ± 0.526
5.415ThrGly: 5.415 ± 0.673
1.66ThrHis: 1.66 ± 0.368
2.271ThrIle: 2.271 ± 0.461
3.057ThrLys: 3.057 ± 0.385
5.241ThrLeu: 5.241 ± 0.692
0.961ThrMet: 0.961 ± 0.29
2.446ThrAsn: 2.446 ± 0.394
2.97ThrPro: 2.97 ± 0.37
3.406ThrGln: 3.406 ± 0.783
2.184ThrArg: 2.184 ± 0.448
2.708ThrSer: 2.708 ± 0.48
2.533ThrThr: 2.533 ± 0.83
4.193ThrVal: 4.193 ± 0.545
0.873ThrTrp: 0.873 ± 0.27
1.572ThrTyr: 1.572 ± 0.39
0.0ThrXaa: 0.0 ± 0.0
Val
3.93ValAla: 3.93 ± 0.543
0.786ValCys: 0.786 ± 0.361
3.581ValAsp: 3.581 ± 0.764
4.542ValGlu: 4.542 ± 0.523
1.572ValPhe: 1.572 ± 0.362
2.882ValGly: 2.882 ± 0.852
1.31ValHis: 1.31 ± 0.315
3.057ValIle: 3.057 ± 0.598
4.455ValLys: 4.455 ± 0.818
4.629ValLeu: 4.629 ± 0.544
1.485ValMet: 1.485 ± 0.338
2.795ValAsn: 2.795 ± 0.488
1.922ValPro: 1.922 ± 0.396
1.747ValGln: 1.747 ± 0.385
2.446ValArg: 2.446 ± 0.444
3.756ValSer: 3.756 ± 0.482
3.843ValThr: 3.843 ± 0.44
2.882ValVal: 2.882 ± 0.589
1.048ValTrp: 1.048 ± 0.306
2.271ValTyr: 2.271 ± 0.459
0.0ValXaa: 0.0 ± 0.0
Trp
1.31TrpAla: 1.31 ± 0.29
0.175TrpCys: 0.175 ± 0.126
0.961TrpAsp: 0.961 ± 0.286
0.611TrpGlu: 0.611 ± 0.203
0.699TrpPhe: 0.699 ± 0.302
0.873TrpGly: 0.873 ± 0.253
0.175TrpHis: 0.175 ± 0.112
0.437TrpIle: 0.437 ± 0.265
1.223TrpLys: 1.223 ± 0.403
2.184TrpLeu: 2.184 ± 0.497
0.349TrpMet: 0.349 ± 0.213
0.611TrpAsn: 0.611 ± 0.229
0.611TrpPro: 0.611 ± 0.228
0.524TrpGln: 0.524 ± 0.221
0.873TrpArg: 0.873 ± 0.283
1.398TrpSer: 1.398 ± 0.281
0.524TrpThr: 0.524 ± 0.19
1.048TrpVal: 1.048 ± 0.323
0.175TrpTrp: 0.175 ± 0.102
0.699TrpTyr: 0.699 ± 0.214
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.882TyrAla: 2.882 ± 0.67
0.262TyrCys: 0.262 ± 0.129
2.97TyrAsp: 2.97 ± 0.593
1.922TyrGlu: 1.922 ± 0.349
1.048TyrPhe: 1.048 ± 0.202
2.533TyrGly: 2.533 ± 0.622
0.699TyrHis: 0.699 ± 0.198
2.096TyrIle: 2.096 ± 0.449
2.009TyrLys: 2.009 ± 0.467
2.446TyrLeu: 2.446 ± 0.598
0.699TyrMet: 0.699 ± 0.207
1.572TyrAsn: 1.572 ± 0.362
1.135TyrPro: 1.135 ± 0.315
1.747TyrGln: 1.747 ± 0.405
2.096TyrArg: 2.096 ± 0.422
2.271TyrSer: 2.271 ± 0.445
1.747TyrThr: 1.747 ± 0.305
1.572TyrVal: 1.572 ± 0.374
0.437TyrTrp: 0.437 ± 0.164
1.223TyrTyr: 1.223 ± 0.321
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (11450 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski