Fig mosaic emaravirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2GAD5|I2GAD5_9VIRU p6 protein OS=Fig mosaic emaravirus OX=1980427 GN=RNA6 PE=4 SV=1
MM1 pKa = 7.35MMRR4 pKa = 11.84AFEE7 pKa = 4.38TIYY10 pKa = 10.43NVCIARR16 pKa = 11.84DD17 pKa = 3.75LDD19 pKa = 3.94SLHH22 pKa = 8.17DD23 pKa = 3.88EE24 pKa = 4.36MEE26 pKa = 4.88CYY28 pKa = 10.46LFNAVADD35 pKa = 4.92KK36 pKa = 10.35IDD38 pKa = 3.42NMEE41 pKa = 4.38SYY43 pKa = 11.22SEE45 pKa = 4.02FLAMRR50 pKa = 11.84DD51 pKa = 3.24AYY53 pKa = 10.62FDD55 pKa = 3.94KK56 pKa = 10.86KK57 pKa = 11.24LDD59 pKa = 3.51MSKK62 pKa = 10.61FNSTIPHH69 pKa = 5.57VSVEE73 pKa = 4.21GCNVSQKK80 pKa = 9.79VIRR83 pKa = 11.84YY84 pKa = 6.83FMRR87 pKa = 11.84YY88 pKa = 6.4VQIVSSLFGSCNILDD103 pKa = 4.36GNLSQDD109 pKa = 3.17FHH111 pKa = 8.52LFGTNVIGNSVSPVIPDD128 pKa = 3.19NSKK131 pKa = 10.26YY132 pKa = 11.24LLMKK136 pKa = 9.02PTDD139 pKa = 3.66VQDD142 pKa = 4.04LVWAVVIGKK151 pKa = 9.64DD152 pKa = 3.82PIEE155 pKa = 4.18TLRR158 pKa = 11.84KK159 pKa = 9.8SQFLQNKK166 pKa = 7.52VATIPTVSEE175 pKa = 4.08EE176 pKa = 4.9LSFLYY181 pKa = 9.8QQYY184 pKa = 10.25HH185 pKa = 6.5ISHH188 pKa = 7.25

Molecular weight: 21.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8Z2H2|C8Z2H2_9VIRU Putative movement protein OS=Fig mosaic emaravirus OX=1980427 GN=RNA4 PE=2 SV=1
MM1 pKa = 7.52APKK4 pKa = 10.48SKK6 pKa = 7.72TTSASSSKK14 pKa = 8.81ITPGQMQEE22 pKa = 3.97NTVLLSSGSKK32 pKa = 10.34LKK34 pKa = 10.64AIKK37 pKa = 8.71LTNVVNGKK45 pKa = 8.44LTHH48 pKa = 7.18PEE50 pKa = 3.87TSDD53 pKa = 3.32LKK55 pKa = 10.83PIDD58 pKa = 3.97VEE60 pKa = 4.5VQAFTSASQNISNFTLHH77 pKa = 7.36KK78 pKa = 9.92YY79 pKa = 10.69RR80 pKa = 11.84NICHH84 pKa = 6.64VDD86 pKa = 3.03TCAAHH91 pKa = 6.7LSKK94 pKa = 10.95SKK96 pKa = 10.35EE97 pKa = 3.94IKK99 pKa = 10.2EE100 pKa = 3.9KK101 pKa = 10.63LQARR105 pKa = 11.84NLRR108 pKa = 11.84LIVSSNEE115 pKa = 3.24FLVVVKK121 pKa = 9.75EE122 pKa = 4.45LNDD125 pKa = 3.57STVDD129 pKa = 3.46NVVSFNKK136 pKa = 10.14ACAIMSAGILKK147 pKa = 8.9HH148 pKa = 5.71TFDD151 pKa = 5.81EE152 pKa = 4.44EE153 pKa = 4.41FDD155 pKa = 3.72WKK157 pKa = 10.59LSKK160 pKa = 10.55YY161 pKa = 10.63VKK163 pKa = 9.75TNNTTKK169 pKa = 10.59VIPDD173 pKa = 3.1VKK175 pKa = 10.24IINRR179 pKa = 11.84LAGQMGLSAGNPYY192 pKa = 10.72YY193 pKa = 10.44WMIVPGYY200 pKa = 8.6EE201 pKa = 4.21FLYY204 pKa = 10.04EE205 pKa = 4.26LYY207 pKa = 9.64PAEE210 pKa = 4.09VLAYY214 pKa = 9.05TLVRR218 pKa = 11.84LQYY221 pKa = 10.38RR222 pKa = 11.84KK223 pKa = 10.08NLNIPDD229 pKa = 4.09SMTDD233 pKa = 3.11ADD235 pKa = 4.01IVSSLVMKK243 pKa = 9.43MNRR246 pKa = 11.84IHH248 pKa = 7.37KK249 pKa = 10.08LEE251 pKa = 3.85QTSFDD256 pKa = 3.66EE257 pKa = 4.47ALNLIGKK264 pKa = 9.61DD265 pKa = 3.69NVSEE269 pKa = 4.58AYY271 pKa = 10.5VEE273 pKa = 3.99LARR276 pKa = 11.84DD277 pKa = 3.33IGSTSKK283 pKa = 9.71TKK285 pKa = 10.84RR286 pKa = 11.84NDD288 pKa = 3.05EE289 pKa = 4.78AILKK293 pKa = 9.56FKK295 pKa = 10.61EE296 pKa = 4.52LIASFLPALEE306 pKa = 4.32ADD308 pKa = 5.16RR309 pKa = 11.84IASAHH314 pKa = 4.4VV315 pKa = 3.04

Molecular weight: 35.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4304

188

2297

717.3

82.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.88 ± 0.768

1.905 ± 0.463

6.808 ± 0.475

5.832 ± 0.175

4.345 ± 0.343

3.415 ± 0.381

2.463 ± 0.276

9.41 ± 0.6

8.434 ± 0.212

9.038 ± 0.699

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.273

6.761 ± 0.499

3.067 ± 0.082

2.718 ± 0.201

3.485 ± 0.427

7.644 ± 0.419

6.064 ± 0.495

5.599 ± 0.812

0.558 ± 0.243

5.901 ± 0.957
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski