Subterranean clover mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8BEQ3|Q8BEQ3_9VIRU ORF1 OS=Subterranean clover mottle virus OX=12472 PE=4 SV=1
MM1 pKa = 7.61PSVSIEE7 pKa = 4.02VYY9 pKa = 9.93SRR11 pKa = 11.84EE12 pKa = 4.11RR13 pKa = 11.84PTLLLLTHH21 pKa = 6.95RR22 pKa = 11.84FWPSSEE28 pKa = 4.18TIPRR32 pKa = 11.84DD33 pKa = 3.39YY34 pKa = 10.95EE35 pKa = 3.87YY36 pKa = 11.05DD37 pKa = 3.5QTVTLYY43 pKa = 11.04GRR45 pKa = 11.84FNNEE49 pKa = 3.46EE50 pKa = 3.99EE51 pKa = 4.22TLILCISRR59 pKa = 11.84CNTCDD64 pKa = 3.68FWCVLGDD71 pKa = 3.8SNLPGIEE78 pKa = 3.88IWDD81 pKa = 3.81SALSCFANAEE91 pKa = 4.17DD92 pKa = 4.54CTLSGQCSACRR103 pKa = 11.84NPPVEE108 pKa = 4.54EE109 pKa = 4.86DD110 pKa = 3.53ADD112 pKa = 3.92SDD114 pKa = 4.28SSEE117 pKa = 4.54EE118 pKa = 3.99YY119 pKa = 10.17QSLGNRR125 pKa = 11.84TVIRR129 pKa = 11.84DD130 pKa = 3.92SGPEE134 pKa = 4.38GYY136 pKa = 9.43WSEE139 pKa = 4.25SSDD142 pKa = 5.2DD143 pKa = 3.86EE144 pKa = 4.45DD145 pKa = 3.5HH146 pKa = 6.66TRR148 pKa = 11.84AAYY151 pKa = 10.0SPCDD155 pKa = 3.49YY156 pKa = 10.73EE157 pKa = 5.04GNLVRR162 pKa = 11.84LFQLQSIQPLQEE174 pKa = 5.04GDD176 pKa = 3.66DD177 pKa = 3.81QEE179 pKa = 4.29

Molecular weight: 20.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8BEQ1|Q8BEQ1_9VIRU N-terminal protein OS=Subterranean clover mottle virus OX=12472 PE=4 SV=1
MM1 pKa = 6.82TGRR4 pKa = 11.84RR5 pKa = 11.84VRR7 pKa = 11.84RR8 pKa = 11.84SKK10 pKa = 10.76KK11 pKa = 9.99KK12 pKa = 10.09SGQEE16 pKa = 3.42TTTQQNRR23 pKa = 11.84QRR25 pKa = 11.84SGGKK29 pKa = 8.99QSQNQMIQVKK39 pKa = 9.55RR40 pKa = 11.84EE41 pKa = 3.99RR42 pKa = 11.84TPMASTVVIGRR53 pKa = 11.84SFPAIHH59 pKa = 6.68SEE61 pKa = 4.03GTRR64 pKa = 11.84TRR66 pKa = 11.84VCHH69 pKa = 6.24TEE71 pKa = 4.39LIRR74 pKa = 11.84AVDD77 pKa = 3.21INTIFTLNFTWCIPSAFPWLSGVAVNWSKK106 pKa = 10.38WRR108 pKa = 11.84WVSLRR113 pKa = 11.84FTYY116 pKa = 9.9MPAAATTTQGTVALGYY132 pKa = 10.73LYY134 pKa = 10.62DD135 pKa = 4.71ALDD138 pKa = 4.13AVPAALQQMSSLAGFSTGAVWSGSEE163 pKa = 4.08GSVLLRR169 pKa = 11.84AANKK173 pKa = 9.88NGNVPGAVSSQLNIADD189 pKa = 3.4PSKK192 pKa = 10.26YY193 pKa = 10.14YY194 pKa = 10.4RR195 pKa = 11.84YY196 pKa = 10.5EE197 pKa = 3.75NLTNFAAIAEE207 pKa = 4.58GTKK210 pKa = 10.52NLYY213 pKa = 10.45APARR217 pKa = 11.84LAVAAANGAADD228 pKa = 3.64VGGVGTVYY236 pKa = 9.77ATYY239 pKa = 10.37VVEE242 pKa = 5.54LIDD245 pKa = 5.24PISSALNSS253 pKa = 3.57

Molecular weight: 27.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1978

179

966

494.5

54.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.128 ± 0.954

1.871 ± 0.501

5.612 ± 0.643

6.775 ± 0.876

3.286 ± 0.282

7.381 ± 0.357

1.82 ± 0.254

4.601 ± 0.338

6.37 ± 1.299

9.454 ± 0.587

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.618 ± 0.148

3.539 ± 0.446

5.157 ± 0.217

2.932 ± 0.539

5.207 ± 0.37

9.505 ± 0.943

5.308 ± 0.816

7.28 ± 0.607

2.123 ± 0.171

3.033 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski