Escherichia phage Halfdan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lokivirus; unclassified Lokivirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5H555|A0A2Z5H555_9CAUD DNA primase/helicase OS=Escherichia phage Halfdan OX=2234092 PE=4 SV=1
MM1 pKa = 7.65PAYY4 pKa = 10.22DD5 pKa = 3.47VTTSFEE11 pKa = 4.21MTDD14 pKa = 3.69GRR16 pKa = 11.84ILEE19 pKa = 4.3CTYY22 pKa = 11.08VLSVTPARR30 pKa = 11.84LWGAPEE36 pKa = 3.53NCYY39 pKa = 10.28PEE41 pKa = 4.63EE42 pKa = 4.97SEE44 pKa = 4.25ASEE47 pKa = 4.6GVFTIDD53 pKa = 4.73GEE55 pKa = 4.41EE56 pKa = 3.71VDD58 pKa = 4.88YY59 pKa = 11.79DD60 pKa = 3.99NLPKK64 pKa = 10.65GLAVIADD71 pKa = 4.16KK72 pKa = 11.35LEE74 pKa = 3.89QAGPGEE80 pKa = 3.99FGYY83 pKa = 10.88NEE85 pKa = 4.4SLRR88 pKa = 11.84EE89 pKa = 3.83PDD91 pKa = 4.16YY92 pKa = 11.55EE93 pKa = 4.67PDD95 pKa = 3.79FYY97 pKa = 11.47DD98 pKa = 3.68YY99 pKa = 11.5DD100 pKa = 3.58

Molecular weight: 11.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5H3F7|A0A2Z5H3F7_9CAUD DNA-directed DNA polymerase OS=Escherichia phage Halfdan OX=2234092 PE=4 SV=1
MM1 pKa = 7.11SRR3 pKa = 11.84SRR5 pKa = 11.84NRR7 pKa = 11.84LAVGKK12 pKa = 10.02LEE14 pKa = 5.82HH15 pKa = 6.2FAAWCEE21 pKa = 3.86KK22 pKa = 10.5QGWKK26 pKa = 9.81RR27 pKa = 11.84HH28 pKa = 4.32QCKK31 pKa = 9.69GAYY34 pKa = 7.94EE35 pKa = 4.3VLRR38 pKa = 11.84MRR40 pKa = 11.84HH41 pKa = 4.9EE42 pKa = 4.31VEE44 pKa = 3.69NDD46 pKa = 3.1TLIVYY51 pKa = 10.2RR52 pKa = 11.84RR53 pKa = 11.84DD54 pKa = 3.18STNAGGEE61 pKa = 4.29LVHH64 pKa = 6.27LTLFGIAEE72 pKa = 4.24RR73 pKa = 11.84TFNRR77 pKa = 11.84YY78 pKa = 8.26IKK80 pKa = 9.87ATRR83 pKa = 3.33

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13674

55

1149

244.2

26.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.926 ± 0.855

1.112 ± 0.182

5.829 ± 0.213

5.726 ± 0.324

3.73 ± 0.193

8.154 ± 0.288

1.265 ± 0.15

5.514 ± 0.235

5.485 ± 0.331

7.518 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.122

5.148 ± 0.327

4.79 ± 0.389

4.212 ± 0.399

4.673 ± 0.287

5.478 ± 0.221

6.275 ± 0.294

6.86 ± 0.276

1.55 ± 0.153

3.481 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski