Georgenia muralis
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3556 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4ZAH6|A0A3N4ZAH6_9MICO Uncharacterized protein (TIGR02679 family) OS=Georgenia muralis OX=154117 GN=EDD32_3631 PE=4 SV=1
MM1 pKa = 7.21 STRR4 pKa = 11.84 KK5 pKa = 8.45 LTAITAATAGALLLASCGGDD25 pKa = 3.06 TGGGTGDD32 pKa = 3.21 ATGGAEE38 pKa = 4.63 GGDD41 pKa = 3.83 DD42 pKa = 4.67 PIVIGISLPLTGDD55 pKa = 3.18 FAEE58 pKa = 5.01 PGKK61 pKa = 10.58 GVQRR65 pKa = 11.84 GYY67 pKa = 8.12 EE68 pKa = 3.83 TWAKK72 pKa = 10.18 VINDD76 pKa = 3.56 GGGLLGRR83 pKa = 11.84 QVEE86 pKa = 4.42 LKK88 pKa = 10.5 ILDD91 pKa = 4.39 DD92 pKa = 4.56 ASDD95 pKa = 3.68 AARR98 pKa = 11.84 VVADD102 pKa = 3.96 YY103 pKa = 10.76 EE104 pKa = 4.39 SLIAQDD110 pKa = 4.03 EE111 pKa = 4.63 VDD113 pKa = 4.27 LVMGPFSTRR122 pKa = 11.84 LVVPASRR129 pKa = 11.84 VADD132 pKa = 4.16 EE133 pKa = 4.21 YY134 pKa = 11.81 GMLFVEE140 pKa = 5.08 PAAAAAEE147 pKa = 4.17 VFEE150 pKa = 5.11 NGFEE154 pKa = 4.47 NIFYY158 pKa = 9.58 AAPAIAPDD166 pKa = 3.66 HH167 pKa = 6.18 YY168 pKa = 11.49 NYY170 pKa = 11.06 LLDD173 pKa = 5.22 FIEE176 pKa = 5.75 AMPEE180 pKa = 3.73 DD181 pKa = 4.95 EE182 pKa = 4.98 RR183 pKa = 11.84 PQTAAVAAMDD193 pKa = 4.7 DD194 pKa = 4.01 PFAQGTAYY202 pKa = 10.51 GLRR205 pKa = 11.84 DD206 pKa = 3.55 GLAEE210 pKa = 4.37 LGVEE214 pKa = 3.91 IVVDD218 pKa = 3.95 EE219 pKa = 4.8 VYY221 pKa = 10.62 PPNATDD227 pKa = 3.47 MSAVAAQIADD237 pKa = 3.77 ADD239 pKa = 4.14 PDD241 pKa = 4.05 VVIGGTQYY249 pKa = 11.2 QDD251 pKa = 3.33 AVNMILALQQLDD263 pKa = 3.88 YY264 pKa = 9.97 QPRR267 pKa = 11.84 MAAFSTAPTNPEE279 pKa = 3.43 FVEE282 pKa = 4.94 AIGGAVDD289 pKa = 5.97 GILSPTGYY297 pKa = 8.26 TVEE300 pKa = 4.0 ATFPSNEE307 pKa = 3.83 EE308 pKa = 3.36 FVAANEE314 pKa = 4.16 EE315 pKa = 4.14 LHH317 pKa = 6.19 GAEE320 pKa = 4.54 PGEE323 pKa = 4.65 DD324 pKa = 3.12 EE325 pKa = 4.84 ANAYY329 pKa = 7.45 TAGQVVAAAVEE340 pKa = 4.14 AVGCAEE346 pKa = 5.59 QGDD349 pKa = 4.4 CQQDD353 pKa = 4.05 LIDD356 pKa = 3.92 WLHH359 pKa = 6.04 EE360 pKa = 4.23 NEE362 pKa = 3.88 VDD364 pKa = 3.94 TVVGTLGWDD373 pKa = 3.23 EE374 pKa = 4.22 AGRR377 pKa = 11.84 PNGAHH382 pKa = 8.12 LIQQYY387 pKa = 10.57 VDD389 pKa = 3.48 GEE391 pKa = 4.2 IAIVLPADD399 pKa = 3.35 VAEE402 pKa = 5.07 AEE404 pKa = 4.56 LIYY407 pKa = 9.76 PKK409 pKa = 10.57 PEE411 pKa = 3.45 WW412 pKa = 3.77
Molecular weight: 43.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.465
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.503
Grimsley 3.363
Solomon 3.643
Lehninger 3.605
Nozaki 3.757
DTASelect 3.961
Thurlkill 3.503
EMBOSS 3.579
Sillero 3.795
Patrickios 1.265
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A3N4Z8D2|A0A3N4Z8D2_9MICO Phosphoribosyl-AMP cyclohydrolase OS=Georgenia muralis OX=154117 GN=hisI PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.79 HH17 pKa = 5.67 RR18 pKa = 11.84 KK19 pKa = 8.31 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3556
0
3556
1205855
30
3017
339.1
36.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.424 ± 0.064
0.528 ± 0.011
6.222 ± 0.034
5.813 ± 0.038
2.495 ± 0.023
9.582 ± 0.044
2.192 ± 0.021
2.97 ± 0.03
1.413 ± 0.022
10.403 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.739 ± 0.018
1.531 ± 0.023
5.955 ± 0.03
2.463 ± 0.022
8.086 ± 0.046
4.732 ± 0.024
6.302 ± 0.036
9.874 ± 0.039
1.451 ± 0.017
1.824 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here