Cellulophaga phage phi46:3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZZR7|R9ZZR7_9CAUD Structural protein OS=Cellulophaga phage phi46:3 OX=1327985 GN=Phi46:3_gp080 PE=4 SV=1
MM1 pKa = 6.84ITLNVFIYY9 pKa = 10.52PEE11 pKa = 4.21DD12 pKa = 5.42DD13 pKa = 2.79ITRR16 pKa = 11.84IEE18 pKa = 4.06NDD20 pKa = 3.22IDD22 pKa = 5.09PITPMDD28 pKa = 3.21QCEE31 pKa = 4.06YY32 pKa = 10.81LEE34 pKa = 4.29CTFYY38 pKa = 11.02QIAYY42 pKa = 8.15VRR44 pKa = 11.84RR45 pKa = 11.84YY46 pKa = 9.85DD47 pKa = 4.23DD48 pKa = 3.58NFAEE52 pKa = 4.38VGSNGEE58 pKa = 4.3SFVVAHH64 pKa = 6.44SMEE67 pKa = 4.3EE68 pKa = 4.03VNMKK72 pKa = 9.88IQLDD76 pKa = 3.8GAIYY80 pKa = 10.66LNN82 pKa = 3.92

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A2X2|S0A2X2_9CAUD Uncharacterized protein OS=Cellulophaga phage phi46:3 OX=1327985 GN=Phi46:3_gp058 PE=4 SV=1
MM1 pKa = 7.6CVLLLWARR9 pKa = 11.84LLAINLLIYY18 pKa = 10.45RR19 pKa = 11.84KK20 pKa = 9.86LRR22 pKa = 11.84FIRR25 pKa = 11.84AIFEE29 pKa = 3.87QPMYY33 pKa = 10.93NRR35 pKa = 11.84AIYY38 pKa = 9.21PSNGVLL44 pKa = 3.82

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

121

0

121

22451

33

1612

185.5

21.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.14 ± 0.227

0.922 ± 0.12

6.561 ± 0.184

7.746 ± 0.356

4.432 ± 0.184

5.75 ± 0.24

1.238 ± 0.122

8.022 ± 0.207

9.363 ± 0.435

7.857 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.177

7.015 ± 0.256

2.766 ± 0.15

3.225 ± 0.19

3.479 ± 0.17

6.922 ± 0.197

6.463 ± 0.325

5.724 ± 0.17

1.042 ± 0.087

4.12 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski