Daedalea quercina L-15889
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12170 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A165LHK6|A0A165LHK6_9APHY Glutathione transferase OS=Daedalea quercina L-15889 OX=1314783 GN=DAEQUDRAFT_732662 PE=3 SV=1
MM1 pKa = 6.94 NTFFGLLIASLCGLTNALYY20 pKa = 9.97 IPVRR24 pKa = 11.84 VNHH27 pKa = 6.94 DD28 pKa = 3.44 AQASSSGTGVHH39 pKa = 6.02 NPAIRR44 pKa = 11.84 LVGSFRR50 pKa = 11.84 QQSDD54 pKa = 3.3 DD55 pKa = 3.76 ANNGSVVNFQDD66 pKa = 3.15 TVYY69 pKa = 9.36 ATNMTIGGQDD79 pKa = 3.47 VLIQLDD85 pKa = 3.98 TGSSDD90 pKa = 2.79 LWVNLDD96 pKa = 3.25 EE97 pKa = 5.71 GEE99 pKa = 4.42 IEE101 pKa = 4.62 FSNTTDD107 pKa = 3.79 LPAQEE112 pKa = 5.2 AYY114 pKa = 10.52 GIGQIAGTIQFANVEE129 pKa = 4.4 LGDD132 pKa = 4.04 NMVPNQAFMNVSNATNFGTIFNDD155 pKa = 4.02 GVRR158 pKa = 11.84 GILGLAFDD166 pKa = 4.9 AGSTIEE172 pKa = 4.09 STITEE177 pKa = 4.46 AYY179 pKa = 10.5 GEE181 pKa = 4.12 DD182 pKa = 3.95 SEE184 pKa = 5.71 LGEE187 pKa = 4.37 SFLTNVFQQDD197 pKa = 3.05 TSKK200 pKa = 10.98 PLFTVQLGRR209 pKa = 11.84 ADD211 pKa = 3.86 DD212 pKa = 4.05 PQYY215 pKa = 8.32 TTEE218 pKa = 4.01 GAFTIGEE225 pKa = 4.27 YY226 pKa = 10.85 LPDD229 pKa = 3.72 YY230 pKa = 8.57 EE231 pKa = 5.17 TIEE234 pKa = 4.78 DD235 pKa = 3.83 MPKK238 pKa = 10.57 LEE240 pKa = 4.4 RR241 pKa = 11.84 FPNNGTGAAPRR252 pKa = 11.84 WSTVMSGMTVNGEE265 pKa = 3.68 PFQFNTSGVPGTPEE279 pKa = 3.89 GSVVAVLDD287 pKa = 3.59 TGFTFPPIPKK297 pKa = 8.86 PAVDD301 pKa = 5.13 AIYY304 pKa = 10.64 GGIDD308 pKa = 3.11 GAYY311 pKa = 9.63 YY312 pKa = 8.59 DD313 pKa = 4.71 TNSSLWIVPCNKK325 pKa = 9.11 ATTLEE330 pKa = 4.24 FQFGEE335 pKa = 4.3 LSVPIHH341 pKa = 6.45 PLDD344 pKa = 3.49 LTTIVQINNTQVCVNTFRR362 pKa = 11.84 PSTFPVNTQFDD373 pKa = 4.78 LVLGDD378 pKa = 4.55 AFLKK382 pKa = 10.56 NVYY385 pKa = 10.7 ASFNYY390 pKa = 10.44 GNMSEE395 pKa = 4.77 DD396 pKa = 3.33 AVPYY400 pKa = 8.99 MQLLPTTDD408 pKa = 2.65 MHH410 pKa = 6.64 AASKK414 pKa = 10.65 EE415 pKa = 3.87 FDD417 pKa = 3.65 SVRR420 pKa = 11.84 SAMYY424 pKa = 10.06 AQAAASAAASSASASAASAYY444 pKa = 9.9 AALVSGSASTACSTAMPTATPLFASAWSSSAPSAVMTDD482 pKa = 3.27 STPAIIASPDD492 pKa = 3.31 LSGLGNGNSSNPTAASSANGSSATDD517 pKa = 3.4 ASSTDD522 pKa = 3.56 PAATDD527 pKa = 3.45 ASSTDD532 pKa = 3.4 GSSATATSDD541 pKa = 2.79 TSTPTSTDD549 pKa = 3.18 DD550 pKa = 3.59 SSASTTSDD558 pKa = 2.79 TSASSPTDD566 pKa = 3.12 SATSSTATSTDD577 pKa = 2.84 SSTDD581 pKa = 3.32 PSATSTDD588 pKa = 3.24 SSTDD592 pKa = 3.32 PSATSTDD599 pKa = 3.24 SSTDD603 pKa = 3.27 PSATSTASAADD614 pKa = 3.86 PNATSTDD621 pKa = 3.5 SSSDD625 pKa = 3.41 TAGTTSTASAADD637 pKa = 3.77 PSATSSGDD645 pKa = 3.25 SSSGGNSSSTGSLPIDD661 pKa = 4.08 GSSSSDD667 pKa = 3.41 DD668 pKa = 3.58 GSGSLQEE675 pKa = 4.22 NTSLLDD681 pKa = 3.34 NSGGSKK687 pKa = 10.3 QSTFGNSLYY696 pKa = 11.08 GDD698 pKa = 4.19 GGNGYY703 pKa = 10.32 ARR705 pKa = 11.84 AFRR708 pKa = 11.84 RR709 pKa = 11.84 SYY711 pKa = 10.93 RR712 pKa = 11.84 EE713 pKa = 3.6 PTKK716 pKa = 10.24 TVHH719 pKa = 7.44 DD720 pKa = 4.78 CLTPMIEE727 pKa = 4.37 EE728 pKa = 4.09 YY729 pKa = 10.93 GALLFALIAGTFIISFALCLVTVVLAIRR757 pKa = 11.84 NHH759 pKa = 5.61 SRR761 pKa = 11.84 SQARR765 pKa = 11.84 KK766 pKa = 7.39 RR767 pKa = 11.84 AEE769 pKa = 4.05 YY770 pKa = 10.05 FVLEE774 pKa = 4.31 ANAEE778 pKa = 4.07 AEE780 pKa = 4.38 ADD782 pKa = 3.76 PEE784 pKa = 4.29 AACTATYY791 pKa = 9.88 HH792 pKa = 6.56 YY793 pKa = 10.92 SDD795 pKa = 5.47 DD796 pKa = 3.97 YY797 pKa = 12.02
Molecular weight: 82.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.478
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.923
Patrickios 1.049
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A165THV8|A0A165THV8_9APHY Uncharacterized protein OS=Daedalea quercina L-15889 OX=1314783 GN=DAEQUDRAFT_721516 PE=4 SV=1
PP1 pKa = 6.32 SARR4 pKa = 11.84 RR5 pKa = 11.84 GRR7 pKa = 11.84 AASPTKK13 pKa = 8.96 PTSTIAPTKK22 pKa = 9.38 RR23 pKa = 11.84 TAPPVKK29 pKa = 9.93 RR30 pKa = 11.84 ATSPTKK36 pKa = 10.42 RR37 pKa = 11.84 ATSPAKK43 pKa = 10.18 RR44 pKa = 11.84 AASPIKK50 pKa = 10.28 RR51 pKa = 11.84 AAPPVKK57 pKa = 10.03 RR58 pKa = 11.84 AASPVKK64 pKa = 10.21 RR65 pKa = 11.84 AASPVKK71 pKa = 10.35 RR72 pKa = 11.84 AGSPVKK78 pKa = 10.32 RR79 pKa = 11.84 AASPVKK85 pKa = 10.21 RR86 pKa = 11.84 AASPVKK92 pKa = 10.27 RR93 pKa = 11.84 AA94 pKa = 3.43
Molecular weight: 9.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.518
IPC2_protein 11.374
IPC_protein 12.954
Toseland 13.13
ProMoST 13.612
Dawson 13.13
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.896
Grimsley 13.173
Solomon 13.612
Lehninger 13.525
Nozaki 13.13
DTASelect 13.115
Thurlkill 13.13
EMBOSS 13.627
Sillero 13.13
Patrickios 12.618
IPC_peptide 13.627
IPC2_peptide 12.603
IPC2.peptide.svr19 9.24
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12169
1
12170
4971741
49
5065
408.5
45.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.359 ± 0.02
1.314 ± 0.009
5.558 ± 0.016
5.908 ± 0.021
3.459 ± 0.014
6.629 ± 0.02
2.565 ± 0.013
4.441 ± 0.014
4.081 ± 0.02
9.172 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.156 ± 0.009
3.012 ± 0.01
6.68 ± 0.029
3.688 ± 0.014
6.769 ± 0.024
8.453 ± 0.035
5.969 ± 0.017
6.621 ± 0.016
1.456 ± 0.009
2.708 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here