Silurus glanis circovirus
Average proteome isoelectric point is 8.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I1TEL3|I1TEL3_9CIRC ATP-dependent helicase Rep OS=Silurus glanis circovirus OX=1126411 GN=Rep PE=3 SV=1
MM1 pKa = 7.91 PKK3 pKa = 9.78 YY4 pKa = 10.12 QKK6 pKa = 10.49 KK7 pKa = 10.37 SPGKK11 pKa = 8.16 EE12 pKa = 3.46 NRR14 pKa = 11.84 SPVQRR19 pKa = 11.84 PKK21 pKa = 10.31 QARR24 pKa = 11.84 PPKK27 pKa = 10.2 KK28 pKa = 8.74 EE29 pKa = 4.09 APHH32 pKa = 5.64 KK33 pKa = 10.6 RR34 pKa = 11.84 YY35 pKa = 10.58 VFTLNNYY42 pKa = 6.09 TTEE45 pKa = 4.01 EE46 pKa = 4.19 YY47 pKa = 11.25 ARR49 pKa = 11.84 IDD51 pKa = 3.5 NVGADD56 pKa = 3.37 GLARR60 pKa = 11.84 YY61 pKa = 7.49 MITGKK66 pKa = 10.22 EE67 pKa = 3.96 VGEE70 pKa = 4.45 NGTPHH75 pKa = 6.3 LQGFINLKK83 pKa = 7.92 VKK85 pKa = 10.44 KK86 pKa = 9.73 RR87 pKa = 11.84 FSQIKK92 pKa = 8.89 EE93 pKa = 3.9 MLGSRR98 pKa = 11.84 CHH100 pKa = 6.22 IEE102 pKa = 3.89 KK103 pKa = 10.8 ARR105 pKa = 11.84 GTDD108 pKa = 3.27 LEE110 pKa = 4.07 NRR112 pKa = 11.84 VYY114 pKa = 10.49 CSKK117 pKa = 10.7 EE118 pKa = 3.69 GSFQEE123 pKa = 4.31 YY124 pKa = 10.05 GSPVGQGKK132 pKa = 10.26 RR133 pKa = 11.84 SDD135 pKa = 3.55 LDD137 pKa = 3.44 AAAEE141 pKa = 4.11 TLRR144 pKa = 11.84 TSLGDD149 pKa = 3.5 LRR151 pKa = 11.84 SVAEE155 pKa = 4.72 LYY157 pKa = 9.71 PSQFIRR163 pKa = 11.84 YY164 pKa = 8.32 GRR166 pKa = 11.84 GLRR169 pKa = 11.84 DD170 pKa = 3.38 YY171 pKa = 11.31 ASVLGLVKK179 pKa = 10.53 PRR181 pKa = 11.84 DD182 pKa = 3.64 FKK184 pKa = 11.93 ANVTVITGPPGCGKK198 pKa = 10.03 SRR200 pKa = 11.84 YY201 pKa = 9.19 AADD204 pKa = 3.77 HH205 pKa = 6.63 ASGTPYY211 pKa = 10.56 YY212 pKa = 10.2 KK213 pKa = 10.28 PRR215 pKa = 11.84 GDD217 pKa = 2.86 WWDD220 pKa = 4.1 GYY222 pKa = 8.5 HH223 pKa = 6.5 TNATVILDD231 pKa = 4.8 DD232 pKa = 4.05 FYY234 pKa = 11.92 GWIKK238 pKa = 10.27 LDD240 pKa = 3.44 EE241 pKa = 4.24 MLRR244 pKa = 11.84 ICDD247 pKa = 4.21 RR248 pKa = 11.84 YY249 pKa = 8.57 PHH251 pKa = 5.45 QVPVKK256 pKa = 9.28 GGYY259 pKa = 7.91 VQFLARR265 pKa = 11.84 DD266 pKa = 3.48 IFITSNKK273 pKa = 8.76 PVEE276 pKa = 4.2 EE277 pKa = 4.19 WFPNCDD283 pKa = 2.87 CSALFRR289 pKa = 11.84 RR290 pKa = 11.84 INVYY294 pKa = 7.92 LTWNDD299 pKa = 2.75 EE300 pKa = 4.39 CFVEE304 pKa = 6.43 RR305 pKa = 11.84 IDD307 pKa = 3.68 TPYY310 pKa = 10.74 EE311 pKa = 3.83 INFF314 pKa = 3.65
Molecular weight: 35.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.493
IPC2_protein 8.448
IPC_protein 8.346
Toseland 8.726
ProMoST 8.799
Dawson 9.151
Bjellqvist 9.121
Wikipedia 9.311
Rodwell 9.253
Grimsley 9.107
Solomon 9.224
Lehninger 9.194
Nozaki 9.194
DTASelect 8.99
Thurlkill 9.063
EMBOSS 9.282
Sillero 9.268
Patrickios 4.355
IPC_peptide 9.224
IPC2_peptide 7.922
IPC2.peptide.svr19 7.741
Protein with the highest isoelectric point:
>tr|I1TEL3|I1TEL3_9CIRC ATP-dependent helicase Rep OS=Silurus glanis circovirus OX=1126411 GN=Rep PE=3 SV=1
MM1 pKa = 7.67 AFRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 TFRR9 pKa = 11.84 RR10 pKa = 11.84 PIRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 MHH17 pKa = 5.71 RR18 pKa = 11.84 RR19 pKa = 11.84 TRR21 pKa = 11.84 GRR23 pKa = 11.84 RR24 pKa = 11.84 MIRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 SRR31 pKa = 11.84 RR32 pKa = 11.84 SRR34 pKa = 11.84 PMGMRR39 pKa = 11.84 SFNFRR44 pKa = 11.84 AIKK47 pKa = 10.13 LDD49 pKa = 3.9 HH50 pKa = 6.81 IKK52 pKa = 10.94 VGGNSSVTGVLTFCLKK68 pKa = 10.65 DD69 pKa = 3.69 FCANFLNWDD78 pKa = 3.7 YY79 pKa = 11.72 YY80 pKa = 10.72 RR81 pKa = 11.84 FNAVVVKK88 pKa = 7.75 FVPQNTAQFDD98 pKa = 3.9 QEE100 pKa = 4.62 GGLSTVCATAVDD112 pKa = 4.19 YY113 pKa = 11.29 DD114 pKa = 4.63 DD115 pKa = 4.37 EE116 pKa = 4.79 SKK118 pKa = 9.26 PTSVRR123 pKa = 11.84 QLMMMQNARR132 pKa = 11.84 TFRR135 pKa = 11.84 TGVGHH140 pKa = 6.28 SRR142 pKa = 11.84 KK143 pKa = 9.12 FKK145 pKa = 10.26 PKK147 pKa = 7.63 MTFSVVEE154 pKa = 4.22 KK155 pKa = 10.01 VDD157 pKa = 3.63 SVTTYY162 pKa = 11.33 APAGLWMGGNRR173 pKa = 11.84 KK174 pKa = 7.67 WWLNTGYY181 pKa = 10.53 SEE183 pKa = 5.18 VKK185 pKa = 10.51 FLGLKK190 pKa = 10.17 YY191 pKa = 10.9 AMQNMSANDD200 pKa = 2.86 IHH202 pKa = 6.46 YY203 pKa = 10.1 QVLIKK208 pKa = 10.71 GYY210 pKa = 10.39 FSFKK214 pKa = 10.68 SPLITAPTVRR224 pKa = 11.84 GEE226 pKa = 4.04 HH227 pKa = 6.09
Molecular weight: 26.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.26
IPC2_protein 10.072
IPC_protein 11.155
Toseland 11.242
ProMoST 11.491
Dawson 11.286
Bjellqvist 11.155
Wikipedia 11.637
Rodwell 11.316
Grimsley 11.33
Solomon 11.623
Lehninger 11.535
Nozaki 11.228
DTASelect 11.155
Thurlkill 11.228
EMBOSS 11.696
Sillero 11.242
Patrickios 11.008
IPC_peptide 11.623
IPC2_peptide 10.511
IPC2.peptide.svr19 8.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
541
227
314
270.5
31.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.73 ± 0.002
1.848 ± 0.344
5.176 ± 0.791
4.436 ± 1.459
5.36 ± 1.103
7.763 ± 0.467
2.033 ± 0.111
4.067 ± 0.642
6.839 ± 0.439
5.915 ± 0.411
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.957 ± 1.521
4.621 ± 0.147
5.176 ± 1.079
3.142 ± 0.038
9.797 ± 1.658
5.73 ± 0.573
5.915 ± 0.74
6.839 ± 0.712
1.664 ± 0.064
4.991 ± 0.958
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here