Rhynchobatus djiddensis polyomavirus 1
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5CVG5|A0A0B5CVG5_9POLY Large T OS=Rhynchobatus djiddensis polyomavirus 1 OX=2170102 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.42 ARR4 pKa = 11.84 GRR6 pKa = 11.84 AFRR9 pKa = 11.84 RR10 pKa = 11.84 QLGLPCLCLNSKK22 pKa = 10.54 PNVDD26 pKa = 4.36 CDD28 pKa = 3.84 VHH30 pKa = 8.06 FCDD33 pKa = 6.01 IDD35 pKa = 5.2 DD36 pKa = 5.04 DD37 pKa = 5.09 SDD39 pKa = 3.44 WDD41 pKa = 3.81 SDD43 pKa = 3.37 EE44 pKa = 5.48 DD45 pKa = 4.73 PIVNQVRR52 pKa = 11.84 KK53 pKa = 10.34 NIFDD57 pKa = 3.66 NYY59 pKa = 8.78 WRR61 pKa = 11.84 DD62 pKa = 3.17 RR63 pKa = 11.84 LMCPEE68 pKa = 4.68 VISDD72 pKa = 3.92 SEE74 pKa = 4.06
Molecular weight: 8.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.43
IPC2_protein 4.393
IPC_protein 4.329
Toseland 4.101
ProMoST 4.431
Dawson 4.329
Bjellqvist 4.558
Wikipedia 4.291
Rodwell 4.151
Grimsley 4.012
Solomon 4.329
Lehninger 4.279
Nozaki 4.444
DTASelect 4.749
Thurlkill 4.164
EMBOSS 4.304
Sillero 4.457
Patrickios 3.821
IPC_peptide 4.317
IPC2_peptide 4.431
IPC2.peptide.svr19 4.401
Protein with the highest isoelectric point:
>tr|A0A0B5CSL6|A0A0B5CSL6_9POLY VP1 OS=Rhynchobatus djiddensis polyomavirus 1 OX=2170102 GN=VP1 PE=3 SV=1
MM1 pKa = 7.13 GAVIAMLAAEE11 pKa = 5.04 GGTLALEE18 pKa = 4.28 EE19 pKa = 4.12 ALGVSMAALEE29 pKa = 4.07 TSAAIEE35 pKa = 4.16 GFAGVGEE42 pKa = 4.17 MMAASGMAGALEE54 pKa = 4.0 EE55 pKa = 4.51 AAMEE59 pKa = 4.25 TLGTQAAQGMLSEE72 pKa = 4.5 SLVGGGGIALATLNLSSGITGIMLSDD98 pKa = 3.26 FHH100 pKa = 8.57 GNGNRR105 pKa = 11.84 FPYY108 pKa = 10.17 LLKK111 pKa = 10.47 EE112 pKa = 4.17 NQEE115 pKa = 4.19 GEE117 pKa = 4.15 IPAVLLSLTKK127 pKa = 10.71 SPIPGKK133 pKa = 9.96 KK134 pKa = 7.55 WGARR138 pKa = 11.84 RR139 pKa = 11.84 FVKK142 pKa = 10.43 KK143 pKa = 10.35 VGYY146 pKa = 8.84 KK147 pKa = 9.69 RR148 pKa = 11.84 KK149 pKa = 9.81 RR150 pKa = 11.84 RR151 pKa = 11.84 VKK153 pKa = 10.36 KK154 pKa = 10.03 RR155 pKa = 11.84 AQPPQEE161 pKa = 3.95 AEE163 pKa = 3.89 EE164 pKa = 4.24 VVEE167 pKa = 4.19 EE168 pKa = 4.09 EE169 pKa = 3.45 RR170 pKa = 11.84 RR171 pKa = 11.84 YY172 pKa = 9.56 PLRR175 pKa = 11.84 RR176 pKa = 11.84 GPLFRR181 pKa = 11.84 RR182 pKa = 11.84 PRR184 pKa = 11.84 EE185 pKa = 3.73 RR186 pKa = 11.84 QPYY189 pKa = 9.28 RR190 pKa = 11.84 EE191 pKa = 4.28 EE192 pKa = 3.9 IEE194 pKa = 4.19 NLLFVSAATSGVRR207 pKa = 11.84 KK208 pKa = 9.34 RR209 pKa = 11.84 RR210 pKa = 11.84 KK211 pKa = 9.05 ARR213 pKa = 11.84 KK214 pKa = 8.19 PRR216 pKa = 11.84 CTRR219 pKa = 11.84 YY220 pKa = 9.93 YY221 pKa = 10.38 PNGNCQEE228 pKa = 4.24 KK229 pKa = 10.26 KK230 pKa = 10.5 GPKK233 pKa = 9.5 GPHH236 pKa = 4.8 SSKK239 pKa = 10.51 RR240 pKa = 11.84 RR241 pKa = 11.84 LL242 pKa = 3.29
Molecular weight: 26.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.205
IPC2_protein 9.399
IPC_protein 9.545
Toseland 10.335
ProMoST 9.926
Dawson 10.467
Bjellqvist 10.101
Wikipedia 10.613
Rodwell 10.877
Grimsley 10.511
Solomon 10.511
Lehninger 10.482
Nozaki 10.321
DTASelect 10.101
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.379
Patrickios 10.57
IPC_peptide 10.511
IPC2_peptide 8.712
IPC2.peptide.svr19 8.509
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1190
74
597
297.5
33.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.387 ± 1.607
2.941 ± 0.939
5.798 ± 1.625
7.647 ± 0.869
4.286 ± 0.595
7.227 ± 1.359
2.101 ± 0.711
5.126 ± 0.612
7.479 ± 0.487
8.655 ± 0.463
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.941 ± 0.395
4.454 ± 0.443
4.79 ± 0.371
2.941 ± 0.052
6.723 ± 1.172
6.723 ± 0.435
4.538 ± 0.73
5.21 ± 0.295
0.84 ± 0.19
3.193 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here