Flanders hapavirus
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A077CZE0|A0A077CZE0_9RHAB SH protein OS=Flanders hapavirus OX=1972612 PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 5.42 LYY4 pKa = 10.74 INIGITFHH12 pKa = 6.52 YY13 pKa = 9.87 NNSYY17 pKa = 10.84 LDD19 pKa = 3.79 NDD21 pKa = 3.93 SLNWIISRR29 pKa = 11.84 MIYY32 pKa = 10.41 DD33 pKa = 4.42 CVRR36 pKa = 11.84 EE37 pKa = 4.06 SGIPGDD43 pKa = 3.77 VAAFAINMAWEE54 pKa = 4.3 HH55 pKa = 6.04 VDD57 pKa = 3.91 ISFDD61 pKa = 3.54 SDD63 pKa = 3.95 KK64 pKa = 10.32 ITHH67 pKa = 6.69 GYY69 pKa = 9.76 CWLQEE74 pKa = 4.02 AVSLPGTPKK83 pKa = 10.94 DD84 pKa = 3.96 LDD86 pKa = 3.63 KK87 pKa = 11.71 LNNTFSSRR95 pKa = 11.84 GNFFIQGDD103 pKa = 3.78 EE104 pKa = 3.93 ALGEE108 pKa = 4.05 VEE110 pKa = 4.55 YY111 pKa = 11.19 VFFVAEE117 pKa = 4.04 PTLQEE122 pKa = 3.82 GRR124 pKa = 11.84 PWNMLWHH131 pKa = 7.16 PIFVDD136 pKa = 3.77 PQAYY140 pKa = 8.81 HH141 pKa = 6.25 VKK143 pKa = 10.44 RR144 pKa = 11.84 NPDD147 pKa = 2.83 VVAYY151 pKa = 9.81 KK152 pKa = 10.56 FGFQHH157 pKa = 7.53 LIYY160 pKa = 10.22 PP161 pKa = 4.71
Molecular weight: 18.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.372
IPC2_protein 4.647
IPC_protein 4.571
Toseland 4.431
ProMoST 4.724
Dawson 4.558
Bjellqvist 4.698
Wikipedia 4.469
Rodwell 4.431
Grimsley 4.342
Solomon 4.558
Lehninger 4.507
Nozaki 4.673
DTASelect 4.889
Thurlkill 4.457
EMBOSS 4.495
Sillero 4.724
Patrickios 2.041
IPC_peptide 4.558
IPC2_peptide 4.711
IPC2.peptide.svr19 4.611
Protein with the highest isoelectric point:
>tr|T2FGJ3|T2FGJ3_9RHAB U2 OS=Flanders hapavirus OX=1972612 GN=U2 PE=4 SV=1
MM1 pKa = 7.37 GFDD4 pKa = 3.03 IGKK7 pKa = 10.11 DD8 pKa = 2.6 IGKK11 pKa = 8.87 PLKK14 pKa = 10.23 EE15 pKa = 4.27 AFDD18 pKa = 4.2 KK19 pKa = 11.12 FGSDD23 pKa = 3.09 IKK25 pKa = 10.51 ITFLTVLNWMKK36 pKa = 10.06 WISIGILIVISIILICKK53 pKa = 8.23 IVKK56 pKa = 9.81 VLFQFGKK63 pKa = 10.38 CLWSGFKK70 pKa = 10.36 CCRR73 pKa = 11.84 KK74 pKa = 9.56 CFKK77 pKa = 10.47 SSKK80 pKa = 8.84 TRR82 pKa = 11.84 AKK84 pKa = 10.41 SSKK87 pKa = 10.45 EE88 pKa = 3.64 KK89 pKa = 10.47 IKK91 pKa = 10.81 LKK93 pKa = 10.37 RR94 pKa = 11.84 ATKK97 pKa = 9.95 MIRR100 pKa = 11.84 NPLRR104 pKa = 11.84 RR105 pKa = 11.84 KK106 pKa = 9.27 KK107 pKa = 10.7 SRR109 pKa = 11.84 IKK111 pKa = 10.4 KK112 pKa = 8.1 VPSVIKK118 pKa = 10.64 LII120 pKa = 4.14
Molecular weight: 13.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.182
IPC2_protein 9.677
IPC_protein 9.648
Toseland 10.965
ProMoST 10.379
Dawson 11.008
Bjellqvist 10.57
Wikipedia 11.096
Rodwell 11.696
Grimsley 11.023
Solomon 11.052
Lehninger 11.052
Nozaki 10.935
DTASelect 10.555
Thurlkill 10.921
EMBOSS 11.33
Sillero 10.935
Patrickios 11.418
IPC_peptide 11.067
IPC2_peptide 9.238
IPC2.peptide.svr19 8.631
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2119
120
618
264.9
30.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.964 ± 0.763
1.888 ± 0.344
6.088 ± 0.641
5.286 ± 0.358
4.578 ± 0.523
5.663 ± 0.58
2.076 ± 0.316
7.881 ± 0.733
7.598 ± 1.063
8.495 ± 0.292
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.548 ± 0.45
5.427 ± 0.482
4.908 ± 0.486
3.398 ± 0.309
4.483 ± 0.413
8.164 ± 0.829
5.05 ± 0.499
5.71 ± 0.572
1.935 ± 0.318
4.861 ± 0.645
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here