Amycolatopsis arida

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5KNM4|A0A1I5KNM4_9PSEU Short-chain dehydrogenase OS=Amycolatopsis arida OX=587909 GN=SAMN05421810_101242 PE=3 SV=1
MM1 pKa = 7.56ARR3 pKa = 11.84RR4 pKa = 11.84IRR6 pKa = 11.84NLLAGAAAACLATTVTGVLGAGTAQAAEE34 pKa = 4.3VTYY37 pKa = 10.56EE38 pKa = 4.13SQTLRR43 pKa = 11.84YY44 pKa = 8.72ICTYY48 pKa = 10.11PLIGNDD54 pKa = 2.92ILDD57 pKa = 3.57VKK59 pKa = 10.62FQFQAPEE66 pKa = 4.26SVPSGGTVQPHH77 pKa = 7.26DD78 pKa = 3.59IVATATVPASVVSLLYY94 pKa = 10.61YY95 pKa = 10.46VGGIDD100 pKa = 4.52GVRR103 pKa = 11.84GTADD107 pKa = 2.87GGVTVTNGSPTDD119 pKa = 3.48VDD121 pKa = 3.52ISDD124 pKa = 4.35LRR126 pKa = 11.84IDD128 pKa = 3.72EE129 pKa = 4.53QFVSDD134 pKa = 4.54PDD136 pKa = 3.56SDD138 pKa = 3.93FTVIAHH144 pKa = 6.02QDD146 pKa = 3.08AGTVVPTITAGEE158 pKa = 4.44PGTLGADD165 pKa = 3.01MGSFFAATVDD175 pKa = 4.04FHH177 pKa = 8.22YY178 pKa = 10.66MNTNPPSWQGPEE190 pKa = 4.41SFEE193 pKa = 4.71CDD195 pKa = 3.67LDD197 pKa = 3.78TSIPQDD203 pKa = 3.26TSFTPDD209 pKa = 3.11MTVTPPATLDD219 pKa = 3.2

Molecular weight: 22.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5YAG6|A0A1I5YAG6_9PSEU Uncharacterized protein OS=Amycolatopsis arida OX=587909 GN=SAMN05421810_10727 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.71QGKK33 pKa = 8.65

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5663

0

5663

1734616

29

5193

306.3

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.958 ± 0.048

0.763 ± 0.008

5.901 ± 0.027

5.733 ± 0.034

2.68 ± 0.018

9.534 ± 0.034

2.25 ± 0.017

2.915 ± 0.019

1.556 ± 0.022

10.619 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.611 ± 0.012

1.64 ± 0.017

6.359 ± 0.036

2.386 ± 0.021

8.935 ± 0.035

4.455 ± 0.021

5.888 ± 0.028

9.391 ± 0.032

1.51 ± 0.016

1.917 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski