Changjiang narna-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KID5|A0A1L3KID5_9VIRU Uncharacterized protein OS=Changjiang narna-like virus 3 OX=1922778 PE=4 SV=1
MM1 pKa = 6.88TRR3 pKa = 11.84NTITHH8 pKa = 5.86HH9 pKa = 5.42TAGGRR14 pKa = 11.84APQLSKK20 pKa = 10.9DD21 pKa = 3.35RR22 pKa = 11.84RR23 pKa = 11.84PRR25 pKa = 11.84RR26 pKa = 11.84VLRR29 pKa = 11.84STPTSADD36 pKa = 3.25LHH38 pKa = 6.12VLLPEE43 pKa = 4.36LVWDD47 pKa = 4.42PVQVARR53 pKa = 11.84LSAKK57 pKa = 10.03GCWRR61 pKa = 11.84KK62 pKa = 10.45ANDD65 pKa = 3.61ALIDD69 pKa = 3.75SFQLLGYY76 pKa = 9.69KK77 pKa = 9.84FEE79 pKa = 5.86NILCPVPPSSSVPEE93 pKa = 3.92LMSILKK99 pKa = 10.01SWTAFWLPHH108 pKa = 5.77MLGDD112 pKa = 3.86DD113 pKa = 3.84TPVQRR118 pKa = 11.84FNPYY122 pKa = 10.82SLITSEE128 pKa = 3.7FRR130 pKa = 11.84KK131 pKa = 10.19FIRR134 pKa = 11.84NRR136 pKa = 11.84VTGGGKK142 pKa = 9.11ARR144 pKa = 11.84KK145 pKa = 9.05YY146 pKa = 10.71RR147 pKa = 11.84IGALLLYY154 pKa = 9.99SKK156 pKa = 10.74RR157 pKa = 11.84LFPSFTVEE165 pKa = 3.84MVRR168 pKa = 11.84EE169 pKa = 4.02KK170 pKa = 11.21VNEE173 pKa = 3.88FSKK176 pKa = 10.79SVARR180 pKa = 11.84IEE182 pKa = 4.25PSVLPRR188 pKa = 11.84KK189 pKa = 9.62RR190 pKa = 11.84RR191 pKa = 11.84MFLEE195 pKa = 3.81IQRR198 pKa = 11.84TLDD201 pKa = 3.39EE202 pKa = 4.58FCPPGEE208 pKa = 4.21EE209 pKa = 4.36MVADD213 pKa = 3.79YY214 pKa = 9.85CKK216 pKa = 10.21PFPPSVSACHH226 pKa = 6.15EE227 pKa = 4.02YY228 pKa = 10.71SRR230 pKa = 11.84QEE232 pKa = 4.0GGLQAYY238 pKa = 9.26IRR240 pKa = 11.84DD241 pKa = 3.92FPLEE245 pKa = 4.27GFLEE249 pKa = 4.52DD250 pKa = 3.81PVVKK254 pKa = 10.56KK255 pKa = 10.48ILQQFRR261 pKa = 11.84LTDD264 pKa = 3.43TDD266 pKa = 3.98LSSNGPLNYY275 pKa = 9.84LWEE278 pKa = 4.45RR279 pKa = 11.84MLRR282 pKa = 11.84HH283 pKa = 6.72LIAEE287 pKa = 5.23AIGEE291 pKa = 4.19LGLPEE296 pKa = 5.26SEE298 pKa = 4.57WKK300 pKa = 10.4PMMVGATGLTEE311 pKa = 3.96PLKK314 pKa = 11.06VRR316 pKa = 11.84IVTKK320 pKa = 10.9AEE322 pKa = 3.72WFLQLLTPVQKK333 pKa = 10.46AWHH336 pKa = 5.77SKK338 pKa = 7.41MRR340 pKa = 11.84EE341 pKa = 3.92HH342 pKa = 7.43PVFQLIGGADD352 pKa = 3.43VEE354 pKa = 4.62DD355 pKa = 4.35ALRR358 pKa = 11.84PMKK361 pKa = 10.36LSKK364 pKa = 10.89GEE366 pKa = 4.04KK367 pKa = 9.46VVSGDD372 pKa = 3.39YY373 pKa = 10.73SAATDD378 pKa = 4.41NIFLTYY384 pKa = 9.56TKK386 pKa = 10.15EE387 pKa = 3.6AAEE390 pKa = 4.15AMLEE394 pKa = 4.01RR395 pKa = 11.84TRR397 pKa = 11.84FQLPEE402 pKa = 4.22SVPSYY407 pKa = 10.56TDD409 pKa = 2.78AFLRR413 pKa = 11.84KK414 pKa = 9.4LVVHH418 pKa = 6.67SLTKK422 pKa = 10.51SVLDD426 pKa = 4.23LKK428 pKa = 11.03GSSPVPITRR437 pKa = 11.84GQMMGHH443 pKa = 6.87ILSFPLLCIINRR455 pKa = 11.84AASCMAVSRR464 pKa = 11.84DD465 pKa = 2.83SFMRR469 pKa = 11.84INGDD473 pKa = 3.58DD474 pKa = 4.02VIFPATKK481 pKa = 9.91KK482 pKa = 10.92VYY484 pKa = 9.27TKK486 pKa = 9.62WKK488 pKa = 9.12SATKK492 pKa = 10.04IVGLEE497 pKa = 3.48FSIGKK502 pKa = 9.67NYY504 pKa = 10.09YY505 pKa = 10.34SRR507 pKa = 11.84DD508 pKa = 3.4LALVNSVYY516 pKa = 9.65CTYY519 pKa = 11.11SKK521 pKa = 11.26EE522 pKa = 3.82KK523 pKa = 10.67DD524 pKa = 2.6RR525 pKa = 11.84WIALDD530 pKa = 3.59VPNVGLLNMPLDD542 pKa = 3.8RR543 pKa = 11.84QVDD546 pKa = 4.33LNNGRR551 pKa = 11.84QILPWEE557 pKa = 3.93QLAQLFRR564 pKa = 11.84EE565 pKa = 4.73FNRR568 pKa = 11.84FSTSDD573 pKa = 2.91THH575 pKa = 6.28TKK577 pKa = 8.62YY578 pKa = 11.12LSMFRR583 pKa = 11.84KK584 pKa = 9.97YY585 pKa = 10.95YY586 pKa = 9.69PILRR590 pKa = 11.84GFPGPFYY597 pKa = 11.32GPVEE601 pKa = 4.18YY602 pKa = 10.35GAFGAPVPPKK612 pKa = 10.69HH613 pKa = 6.44EE614 pKa = 4.09FTKK617 pKa = 10.75NQLMWMNAHH626 pKa = 7.17RR627 pKa = 11.84LGIFNYY633 pKa = 10.11QEE635 pKa = 3.8GTRR638 pKa = 11.84NSFSKK643 pKa = 10.34ISNRR647 pKa = 11.84YY648 pKa = 7.79EE649 pKa = 3.75SFIAIEE655 pKa = 4.22LTKK658 pKa = 10.74GMYY661 pKa = 10.2KK662 pKa = 10.67FGPLPVGAAFGPPRR676 pKa = 11.84AADD679 pKa = 4.71GILDD683 pKa = 4.15PYY685 pKa = 11.12ARR687 pKa = 11.84DD688 pKa = 3.16GGLGYY693 pKa = 10.99RR694 pKa = 11.84LMAMRR699 pKa = 11.84RR700 pKa = 11.84WFEE703 pKa = 4.0DD704 pKa = 3.32LSSNKK709 pKa = 9.26HH710 pKa = 4.39VKK712 pKa = 10.1IFGARR717 pKa = 11.84RR718 pKa = 11.84WNHH721 pKa = 5.79FKK723 pKa = 11.17LSMKK727 pKa = 10.11DD728 pKa = 3.09QGGIPPLPPNFLHH741 pKa = 7.15KK742 pKa = 10.75VLEE745 pKa = 4.22NSTWSMRR752 pKa = 11.84PAWHH756 pKa = 6.71RR757 pKa = 11.84QRR759 pKa = 11.84DD760 pKa = 4.2TVGVRR765 pKa = 11.84YY766 pKa = 10.03KK767 pKa = 10.64EE768 pKa = 4.12DD769 pKa = 3.15ASFLHH774 pKa = 7.11EE775 pKa = 4.12IFQAQEE781 pKa = 3.79EE782 pKa = 4.49QTEE785 pKa = 4.44EE786 pKa = 3.98TTLL789 pKa = 3.55

Molecular weight: 90.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KID5|A0A1L3KID5_9VIRU Uncharacterized protein OS=Changjiang narna-like virus 3 OX=1922778 PE=4 SV=1
MM1 pKa = 7.75AKK3 pKa = 9.78TNTKK7 pKa = 10.36KK8 pKa = 10.53SGKK11 pKa = 10.17KK12 pKa = 8.99NVKK15 pKa = 9.89NSNTGNKK22 pKa = 8.5PRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84TMGGGSVAGRR36 pKa = 11.84YY37 pKa = 8.31AQLLHH42 pKa = 6.66NPDD45 pKa = 4.05NGDD48 pKa = 3.26HH49 pKa = 6.28MFDD52 pKa = 3.39VYY54 pKa = 11.02DD55 pKa = 3.82GEE57 pKa = 4.49RR58 pKa = 11.84GEE60 pKa = 4.35TQKK63 pKa = 10.63FVSTITLNTIAGHH76 pKa = 4.98TAGFLQLFGATGNGQWQSGASSSAALTFALLNTGCPGAAYY116 pKa = 8.48LTANAAKK123 pKa = 10.08SRR125 pKa = 11.84CKK127 pKa = 10.31ALKK130 pKa = 10.67LEE132 pKa = 5.59LIPAAASFSNITGEE146 pKa = 4.09VAAGVTTSGSWASGVTSVDD165 pKa = 3.21QLFDD169 pKa = 2.98IAKK172 pKa = 10.04AYY174 pKa = 10.34GPLRR178 pKa = 11.84RR179 pKa = 11.84EE180 pKa = 4.22TVVSRR185 pKa = 11.84WIPSGLDD192 pKa = 3.02HH193 pKa = 7.33TYY195 pKa = 11.59ANYY198 pKa = 10.87NSVPNEE204 pKa = 4.04DD205 pKa = 3.64SNAVYY210 pKa = 9.89IAYY213 pKa = 9.86RR214 pKa = 11.84GWPAGVPISIRR225 pKa = 11.84MTYY228 pKa = 9.78VVEE231 pKa = 4.19FTIKK235 pKa = 10.49NSIGIPPTGAVSVPVGHH252 pKa = 5.96PHH254 pKa = 6.92ILAAMQQQDD263 pKa = 4.06PHH265 pKa = 5.09WHH267 pKa = 6.22HH268 pKa = 7.13SILDD272 pKa = 3.64EE273 pKa = 4.3VKK275 pKa = 9.74RR276 pKa = 11.84AGVGVARR283 pKa = 11.84DD284 pKa = 3.39VGNFTRR290 pKa = 11.84HH291 pKa = 4.31MARR294 pKa = 11.84NGMVRR299 pKa = 11.84VAEE302 pKa = 4.4KK303 pKa = 10.31VFSRR307 pKa = 11.84GVSSAIVLAA316 pKa = 4.61

Molecular weight: 33.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1105

316

789

552.5

61.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.783 ± 2.025

0.905 ± 0.14

4.163 ± 0.352

5.068 ± 1.145

4.796 ± 0.841

7.421 ± 1.558

2.534 ± 0.325

4.525 ± 0.114

5.701 ± 0.492

8.959 ± 1.683

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.351

4.163 ± 0.954

6.244 ± 0.935

3.077 ± 0.118

6.878 ± 0.773

7.33 ± 0.463

5.701 ± 0.813

6.697 ± 0.953

1.9 ± 0.164

3.258 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski