Haloarculaceae archaeon HArcel1

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; unclassified Haloarculaceae

Average proteome isoelectric point is 4.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2532 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4X102|A0A2R4X102_9EURY Uncharacterized protein OS=Haloarculaceae archaeon HArcel1 OX=1679096 GN=HARCEL1_06995 PE=4 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84SPEE5 pKa = 3.75VDD7 pKa = 3.13RR8 pKa = 11.84QQVARR13 pKa = 11.84MQQSPTRR20 pKa = 11.84RR21 pKa = 11.84QILRR25 pKa = 11.84ASGATILGGSVLTAGTHH42 pKa = 4.47TVTAHH47 pKa = 7.27RR48 pKa = 11.84STDD51 pKa = 3.0DD52 pKa = 2.94WHH54 pKa = 6.9QFGYY58 pKa = 11.22DD59 pKa = 3.58DD60 pKa = 5.93ANTGHH65 pKa = 7.35AADD68 pKa = 3.81TTGPVTGVSPEE79 pKa = 3.5WHH81 pKa = 6.55FPDD84 pKa = 4.0VSGLEE89 pKa = 4.13SSPTADD95 pKa = 3.3GDD97 pKa = 4.13TVYY100 pKa = 10.84LGTVRR105 pKa = 11.84GVVALSEE112 pKa = 4.33ADD114 pKa = 3.4RR115 pKa = 11.84SVRR118 pKa = 11.84WRR120 pKa = 11.84TRR122 pKa = 11.84LSSPVKK128 pKa = 7.52TTPVLANGTLYY139 pKa = 11.04VGTADD144 pKa = 3.66GTVCAVDD151 pKa = 3.67PASGAVQWRR160 pKa = 11.84FEE162 pKa = 4.34TNARR166 pKa = 11.84MYY168 pKa = 9.48FSPVVSDD175 pKa = 3.1GTVYY179 pKa = 10.71VGSGDD184 pKa = 3.52ASVYY188 pKa = 11.08ALDD191 pKa = 3.95AVEE194 pKa = 5.52GTEE197 pKa = 3.64LWQYY201 pKa = 7.02EE202 pKa = 4.35TGAEE206 pKa = 4.34VHH208 pKa = 6.01CTPAVADD215 pKa = 3.62GTVYY219 pKa = 10.94VGGIDD224 pKa = 3.66NNVYY228 pKa = 10.83ALDD231 pKa = 4.37AGDD234 pKa = 5.21GTVEE238 pKa = 4.02WQFEE242 pKa = 4.47TEE244 pKa = 4.17DD245 pKa = 3.72TVMGSPAIADD255 pKa = 3.43GTVYY259 pKa = 10.65VGSFDD264 pKa = 3.58NTVYY268 pKa = 10.91AVDD271 pKa = 3.43ASGGSEE277 pKa = 3.66EE278 pKa = 4.15WRR280 pKa = 11.84FDD282 pKa = 2.96TGDD285 pKa = 3.56RR286 pKa = 11.84VNSSPAVADD295 pKa = 3.38GTVFVGSFDD304 pKa = 4.11NNVYY308 pKa = 10.92ALDD311 pKa = 3.93ARR313 pKa = 11.84DD314 pKa = 4.08GAVEE318 pKa = 3.96WRR320 pKa = 11.84FEE322 pKa = 3.76TDD324 pKa = 2.5DD325 pKa = 3.85WVNSSPVIADD335 pKa = 3.35GTVHH339 pKa = 6.13VGSYY343 pKa = 10.52DD344 pKa = 3.58GNVYY348 pKa = 10.87ALDD351 pKa = 3.83RR352 pKa = 11.84THH354 pKa = 7.06GAVKK358 pKa = 9.6WRR360 pKa = 11.84FEE362 pKa = 3.95TGGWEE367 pKa = 3.98RR368 pKa = 11.84SQSAVADD375 pKa = 4.09GTISPSVRR383 pKa = 11.84DD384 pKa = 3.31STVYY388 pKa = 10.73VGTQNSVYY396 pKa = 10.62AIDD399 pKa = 4.01AANGTEE405 pKa = 3.53RR406 pKa = 11.84WQYY409 pKa = 8.39EE410 pKa = 3.93TGAPNDD416 pKa = 3.85SSPAAVDD423 pKa = 3.33GTVYY427 pKa = 10.78LGSTNSRR434 pKa = 11.84VSAIDD439 pKa = 3.62AGDD442 pKa = 3.81GTVQWQFEE450 pKa = 4.48TGDD453 pKa = 3.96EE454 pKa = 4.43VHH456 pKa = 6.85SSPAVDD462 pKa = 4.88DD463 pKa = 3.89GTVYY467 pKa = 10.37IGSWDD472 pKa = 3.54NRR474 pKa = 11.84LYY476 pKa = 11.25ALDD479 pKa = 4.15VDD481 pKa = 5.1DD482 pKa = 6.4GTIQWRR488 pKa = 11.84FEE490 pKa = 3.95TDD492 pKa = 3.13GEE494 pKa = 4.83LNCSPVVADD503 pKa = 3.29GTVYY507 pKa = 10.68VGSLDD512 pKa = 3.54NHH514 pKa = 6.61VYY516 pKa = 10.6AVDD519 pKa = 3.98ADD521 pKa = 4.24DD522 pKa = 3.9GTEE525 pKa = 3.42RR526 pKa = 11.84WRR528 pKa = 11.84YY529 pKa = 5.17EE530 pKa = 3.93TGDD533 pKa = 3.76EE534 pKa = 4.36VHH536 pKa = 6.85SSPAIVDD543 pKa = 3.38GTVYY547 pKa = 10.54VGSWDD552 pKa = 3.64DD553 pKa = 3.65SIYY556 pKa = 10.87AIDD559 pKa = 4.53AGDD562 pKa = 3.7GTVQWRR568 pKa = 11.84YY569 pKa = 6.17EE570 pKa = 4.14TTGGVSSSPAVANDD584 pKa = 3.46TVYY587 pKa = 10.94VGNFEE592 pKa = 4.69GIVYY596 pKa = 10.65ALDD599 pKa = 4.27AEE601 pKa = 4.79DD602 pKa = 6.07GSVEE606 pKa = 3.82WGQTTNGVINSSPAIADD623 pKa = 3.42TTVYY627 pKa = 10.05IGSWDD632 pKa = 3.59NTVYY636 pKa = 11.21ALDD639 pKa = 4.85ADD641 pKa = 4.75DD642 pKa = 4.14GTKK645 pKa = 9.74RR646 pKa = 11.84WEE648 pKa = 3.85YY649 pKa = 9.73RR650 pKa = 11.84TDD652 pKa = 3.97DD653 pKa = 4.91EE654 pKa = 4.63IHH656 pKa = 6.71SSPAVADD663 pKa = 3.49GTVYY667 pKa = 10.71VGSDD671 pKa = 3.64DD672 pKa = 4.28DD673 pKa = 4.65SVYY676 pKa = 11.37ALDD679 pKa = 4.06ATDD682 pKa = 5.49GTVQWQYY689 pKa = 9.74EE690 pKa = 4.23TGDD693 pKa = 3.72WVPQSPSVADD703 pKa = 3.4GTVYY707 pKa = 10.32IGSFDD712 pKa = 3.62GLVYY716 pKa = 11.0ALTEE720 pKa = 4.15PARR723 pKa = 11.84DD724 pKa = 3.71TPSPAVEE731 pKa = 4.09PTEE734 pKa = 4.09TAEE737 pKa = 4.46PRR739 pKa = 11.84QQDD742 pKa = 3.86TEE744 pKa = 4.33GSGIDD749 pKa = 4.12ALPIAAGLGLIGLGGGGGALWWVRR773 pKa = 11.84QQ774 pKa = 3.57

Molecular weight: 82.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4X1I0|A0A2R4X1I0_9EURY Uncharacterized protein OS=Haloarculaceae archaeon HArcel1 OX=1679096 GN=HARCEL1_07955 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84ISTAVSWLVVCAGVAALGAVVWTDD26 pKa = 3.16RR27 pKa = 11.84VARR30 pKa = 11.84AVVPRR35 pKa = 11.84AVGAGLLALLVGVTLGRR52 pKa = 11.84LASTRR57 pKa = 11.84TRR59 pKa = 11.84QASTGLGAPRR69 pKa = 11.84VWPVIVIGGVLGSAVLATIGALGGGLVALPIVEE102 pKa = 4.72APLTVEE108 pKa = 4.57ALRR111 pKa = 11.84TTPLVRR117 pKa = 11.84RR118 pKa = 11.84AALAGVVLTHH128 pKa = 6.96ALAGAAGLTTLAHH141 pKa = 7.0TITPGDD147 pKa = 3.35RR148 pKa = 11.84VAATGPAISLAAIAAAGLALAPIGTVPMAGLTVALGALGVGLYY191 pKa = 10.48ARR193 pKa = 11.84APIRR197 pKa = 11.84ALLDD201 pKa = 3.46RR202 pKa = 11.84LGLDD206 pKa = 3.4ARR208 pKa = 11.84VLPARR213 pKa = 11.84SEE215 pKa = 4.16WAHH218 pKa = 6.55DD219 pKa = 3.5AADD222 pKa = 3.45AEE224 pKa = 4.54RR225 pKa = 11.84AHH227 pKa = 7.2RR228 pKa = 11.84RR229 pKa = 11.84AQKK232 pKa = 10.07RR233 pKa = 11.84APPLDD238 pKa = 3.43IGEE241 pKa = 4.37TTSIVVQEE249 pKa = 4.22RR250 pKa = 11.84LDD252 pKa = 3.48GGRR255 pKa = 11.84QLRR258 pKa = 11.84GVYY261 pKa = 10.26EE262 pKa = 4.2DD263 pKa = 3.62FQVFVKK269 pKa = 10.18WDD271 pKa = 3.75VPPDD275 pKa = 3.66CATGEE280 pKa = 4.45TICVRR285 pKa = 11.84IEE287 pKa = 4.23DD288 pKa = 4.44YY289 pKa = 9.47GTNDD293 pKa = 2.76AGQRR297 pKa = 11.84TSGQARR303 pKa = 11.84FLSRR307 pKa = 11.84GACC310 pKa = 3.33

Molecular weight: 31.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2532

0

2532

790179

36

3324

312.1

33.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.728 ± 0.082

0.69 ± 0.015

9.215 ± 0.059

7.564 ± 0.061

2.916 ± 0.027

8.468 ± 0.049

2.009 ± 0.02

4.448 ± 0.036

1.385 ± 0.026

8.485 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.621 ± 0.021

2.012 ± 0.036

4.962 ± 0.036

2.455 ± 0.03

6.961 ± 0.056

5.545 ± 0.042

6.776 ± 0.055

9.14 ± 0.048

1.188 ± 0.022

2.431 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski