Enterobacteria phage SP (Bacteriophage SP)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Allassoviricetes; Levivirales; Leviviridae; Allolevivirus; Escherichia virus FI

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P09677|A1_BPSP Minor capsid protein A1 OS=Enterobacteria phage SP OX=12027 PE=2 SV=1
MM1 pKa = 7.71AKK3 pKa = 10.19LNQVTLSKK11 pKa = 10.24IGKK14 pKa = 9.4NGDD17 pKa = 3.22QTLTLTPRR25 pKa = 11.84GVNPTNGVASLSEE38 pKa = 4.3AGAVPALEE46 pKa = 4.02KK47 pKa = 10.58RR48 pKa = 11.84VTVSVAQPSRR58 pKa = 11.84NRR60 pKa = 11.84KK61 pKa = 7.49NFKK64 pKa = 10.37VQIKK68 pKa = 9.11LQNPTACTRR77 pKa = 11.84DD78 pKa = 3.24ACDD81 pKa = 3.43PSVTRR86 pKa = 11.84SAFADD91 pKa = 3.47VTLSFTSYY99 pKa = 9.59STDD102 pKa = 3.16EE103 pKa = 3.89EE104 pKa = 4.2RR105 pKa = 11.84ALIRR109 pKa = 11.84TEE111 pKa = 3.98LAALLADD118 pKa = 4.35PLIVDD123 pKa = 5.92AIDD126 pKa = 3.96NLNPAYY132 pKa = 9.34WAALLVASSGGGDD145 pKa = 3.58NPSDD149 pKa = 3.72PDD151 pKa = 3.77VPVVPDD157 pKa = 3.66VKK159 pKa = 10.85PPDD162 pKa = 3.18GTGRR166 pKa = 11.84YY167 pKa = 7.96KK168 pKa = 10.95CPFACYY174 pKa = 9.56RR175 pKa = 11.84LGSIYY180 pKa = 10.43EE181 pKa = 4.09VGKK184 pKa = 10.57EE185 pKa = 4.09GSPDD189 pKa = 2.68IYY191 pKa = 11.07EE192 pKa = 4.63RR193 pKa = 11.84GDD195 pKa = 3.62EE196 pKa = 4.15VSVTFDD202 pKa = 3.26YY203 pKa = 11.3ALEE206 pKa = 4.6DD207 pKa = 4.09FLGNTNWRR215 pKa = 11.84NWDD218 pKa = 3.54QRR220 pKa = 11.84LSDD223 pKa = 3.69YY224 pKa = 11.14DD225 pKa = 3.03IANRR229 pKa = 11.84RR230 pKa = 11.84RR231 pKa = 11.84CRR233 pKa = 11.84GNGYY237 pKa = 9.75IDD239 pKa = 4.98LDD241 pKa = 3.78ATAMQSDD248 pKa = 4.34DD249 pKa = 3.54FVLSGRR255 pKa = 11.84YY256 pKa = 8.51GVRR259 pKa = 11.84KK260 pKa = 10.15VKK262 pKa = 10.62FPGAFGSIKK271 pKa = 10.58YY272 pKa = 10.02LLNIQGDD279 pKa = 3.36AWLDD283 pKa = 3.65LSEE286 pKa = 4.15VTAYY290 pKa = 10.4RR291 pKa = 11.84SYY293 pKa = 11.7GMVIGFWTDD302 pKa = 2.71SKK304 pKa = 11.46SPQLPTDD311 pKa = 4.28FTQFNSANCPVQTVIIIPSLL331 pKa = 3.49

Molecular weight: 36.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P09677|A1_BPSP Minor capsid protein A1 OS=Enterobacteria phage SP OX=12027 PE=2 SV=1
MM1 pKa = 7.17PTLPRR6 pKa = 11.84GLRR9 pKa = 11.84FGSNGEE15 pKa = 3.97VLNDD19 pKa = 4.97FEE21 pKa = 5.65ALWFPEE27 pKa = 3.68RR28 pKa = 11.84HH29 pKa = 5.32TVDD32 pKa = 4.7LSNGTCKK39 pKa = 9.99LTGYY43 pKa = 8.13ITNLPGYY50 pKa = 10.65SDD52 pKa = 3.66IFPNKK57 pKa = 9.32GVTAARR63 pKa = 11.84TPYY66 pKa = 10.4RR67 pKa = 11.84STVPVNHH74 pKa = 6.82LGYY77 pKa = 10.5RR78 pKa = 11.84PVTTVEE84 pKa = 4.23YY85 pKa = 10.01IPDD88 pKa = 3.63GTYY91 pKa = 10.56VRR93 pKa = 11.84LDD95 pKa = 3.21GHH97 pKa = 6.06VKK99 pKa = 10.68FEE101 pKa = 4.2GDD103 pKa = 3.64LVNGSVDD110 pKa = 3.31LTNFVISLAAQGGFDD125 pKa = 3.92YY126 pKa = 11.11QSVIGPRR133 pKa = 11.84FSARR137 pKa = 11.84FSAFSTKK144 pKa = 10.58YY145 pKa = 10.48GVLLGEE151 pKa = 4.26GRR153 pKa = 11.84EE154 pKa = 4.09TLKK157 pKa = 11.12YY158 pKa = 10.15LLLVVRR164 pKa = 11.84RR165 pKa = 11.84MRR167 pKa = 11.84EE168 pKa = 3.72GYY170 pKa = 9.2RR171 pKa = 11.84AVRR174 pKa = 11.84RR175 pKa = 11.84GDD177 pKa = 3.48LKK179 pKa = 10.75RR180 pKa = 11.84LRR182 pKa = 11.84NVISTFEE189 pKa = 3.9PSTIKK194 pKa = 10.57GKK196 pKa = 9.62RR197 pKa = 11.84ARR199 pKa = 11.84AEE201 pKa = 3.83FSQTYY206 pKa = 9.68RR207 pKa = 11.84DD208 pKa = 3.74KK209 pKa = 10.98LTGNKK214 pKa = 9.44VEE216 pKa = 4.12VRR218 pKa = 11.84PSEE221 pKa = 4.3GKK223 pKa = 9.22WNSSSASDD231 pKa = 3.0LWLEE235 pKa = 3.89FRR237 pKa = 11.84YY238 pKa = 10.92GLMPLFYY245 pKa = 10.26DD246 pKa = 3.45IQSVMEE252 pKa = 4.32DD253 pKa = 3.16FMRR256 pKa = 11.84VHH258 pKa = 6.76KK259 pKa = 10.46KK260 pKa = 8.56IAKK263 pKa = 8.24IQRR266 pKa = 11.84FSAGHH271 pKa = 6.04GKK273 pKa = 10.43LEE275 pKa = 4.44TVSSRR280 pKa = 11.84FYY282 pKa = 10.62PDD284 pKa = 2.75VHH286 pKa = 6.75FSLEE290 pKa = 4.03VTAVLQRR297 pKa = 11.84RR298 pKa = 11.84HH299 pKa = 5.06RR300 pKa = 11.84WGVIYY305 pKa = 10.45QDD307 pKa = 3.34TGSFATFNNGRR318 pKa = 11.84LVPVKK323 pKa = 10.05DD324 pKa = 3.07WKK326 pKa = 9.34TAAFALLNPAEE337 pKa = 4.61VAWEE341 pKa = 3.92VTPYY345 pKa = 11.07SFVVDD350 pKa = 3.11WFVNVGDD357 pKa = 4.51MLEE360 pKa = 4.16QMGQLYY366 pKa = 9.74RR367 pKa = 11.84HH368 pKa = 6.16VDD370 pKa = 3.45VVDD373 pKa = 3.84GFDD376 pKa = 4.27RR377 pKa = 11.84KK378 pKa = 10.39DD379 pKa = 3.13IKK381 pKa = 10.88LKK383 pKa = 9.86SVSVRR388 pKa = 11.84VLTNDD393 pKa = 3.15VAHH396 pKa = 6.1VASFQLRR403 pKa = 11.84QAKK406 pKa = 9.53LLHH409 pKa = 6.21SYY411 pKa = 9.86YY412 pKa = 10.93SRR414 pKa = 11.84VHH416 pKa = 5.56TVAFPQISPQLDD428 pKa = 3.44TEE430 pKa = 4.2IRR432 pKa = 11.84SVKK435 pKa = 10.36HH436 pKa = 5.94VIDD439 pKa = 4.9SIALLTQRR447 pKa = 11.84VKK449 pKa = 10.89RR450 pKa = 3.95

Molecular weight: 51.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1489

132

576

372.3

41.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.455 ± 0.726

1.276 ± 0.386

6.985 ± 0.589

4.634 ± 0.516

4.365 ± 0.492

6.514 ± 0.376

1.41 ± 0.5

4.835 ± 0.682

4.835 ± 0.169

9.604 ± 0.449

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.209 ± 0.128

4.231 ± 0.481

5.037 ± 0.404

2.821 ± 0.326

7.79 ± 0.558

7.052 ± 0.368

6.38 ± 0.442

8.328 ± 0.941

1.545 ± 0.196

3.694 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski