Enterobacteria phage SP (Bacteriophage SP)
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P09677|A1_BPSP Minor capsid protein A1 OS=Enterobacteria phage SP OX=12027 PE=2 SV=1
MM1 pKa = 7.71 AKK3 pKa = 10.19 LNQVTLSKK11 pKa = 10.24 IGKK14 pKa = 9.4 NGDD17 pKa = 3.22 QTLTLTPRR25 pKa = 11.84 GVNPTNGVASLSEE38 pKa = 4.3 AGAVPALEE46 pKa = 4.02 KK47 pKa = 10.58 RR48 pKa = 11.84 VTVSVAQPSRR58 pKa = 11.84 NRR60 pKa = 11.84 KK61 pKa = 7.49 NFKK64 pKa = 10.37 VQIKK68 pKa = 9.11 LQNPTACTRR77 pKa = 11.84 DD78 pKa = 3.24 ACDD81 pKa = 3.43 PSVTRR86 pKa = 11.84 SAFADD91 pKa = 3.47 VTLSFTSYY99 pKa = 9.59 STDD102 pKa = 3.16 EE103 pKa = 3.89 EE104 pKa = 4.2 RR105 pKa = 11.84 ALIRR109 pKa = 11.84 TEE111 pKa = 3.98 LAALLADD118 pKa = 4.35 PLIVDD123 pKa = 5.92 AIDD126 pKa = 3.96 NLNPAYY132 pKa = 9.34 WAALLVASSGGGDD145 pKa = 3.58 NPSDD149 pKa = 3.72 PDD151 pKa = 3.77 VPVVPDD157 pKa = 3.66 VKK159 pKa = 10.85 PPDD162 pKa = 3.18 GTGRR166 pKa = 11.84 YY167 pKa = 7.96 KK168 pKa = 10.95 CPFACYY174 pKa = 9.56 RR175 pKa = 11.84 LGSIYY180 pKa = 10.43 EE181 pKa = 4.09 VGKK184 pKa = 10.57 EE185 pKa = 4.09 GSPDD189 pKa = 2.68 IYY191 pKa = 11.07 EE192 pKa = 4.63 RR193 pKa = 11.84 GDD195 pKa = 3.62 EE196 pKa = 4.15 VSVTFDD202 pKa = 3.26 YY203 pKa = 11.3 ALEE206 pKa = 4.6 DD207 pKa = 4.09 FLGNTNWRR215 pKa = 11.84 NWDD218 pKa = 3.54 QRR220 pKa = 11.84 LSDD223 pKa = 3.69 YY224 pKa = 11.14 DD225 pKa = 3.03 IANRR229 pKa = 11.84 RR230 pKa = 11.84 RR231 pKa = 11.84 CRR233 pKa = 11.84 GNGYY237 pKa = 9.75 IDD239 pKa = 4.98 LDD241 pKa = 3.78 ATAMQSDD248 pKa = 4.34 DD249 pKa = 3.54 FVLSGRR255 pKa = 11.84 YY256 pKa = 8.51 GVRR259 pKa = 11.84 KK260 pKa = 10.15 VKK262 pKa = 10.62 FPGAFGSIKK271 pKa = 10.58 YY272 pKa = 10.02 LLNIQGDD279 pKa = 3.36 AWLDD283 pKa = 3.65 LSEE286 pKa = 4.15 VTAYY290 pKa = 10.4 RR291 pKa = 11.84 SYY293 pKa = 11.7 GMVIGFWTDD302 pKa = 2.71 SKK304 pKa = 11.46 SPQLPTDD311 pKa = 4.28 FTQFNSANCPVQTVIIIPSLL331 pKa = 3.49
Molecular weight: 36.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.898
IPC2_protein 4.991
IPC_protein 4.914
Toseland 4.711
ProMoST 5.041
Dawson 4.876
Bjellqvist 5.029
Wikipedia 4.787
Rodwell 4.736
Grimsley 4.622
Solomon 4.876
Lehninger 4.825
Nozaki 4.978
DTASelect 5.232
Thurlkill 4.736
EMBOSS 4.8
Sillero 5.016
Patrickios 4.024
IPC_peptide 4.876
IPC2_peptide 5.003
IPC2.peptide.svr19 5.07
Protein with the highest isoelectric point:
>sp|P09677|A1_BPSP Minor capsid protein A1 OS=Enterobacteria phage SP OX=12027 PE=2 SV=1
MM1 pKa = 7.17 PTLPRR6 pKa = 11.84 GLRR9 pKa = 11.84 FGSNGEE15 pKa = 3.97 VLNDD19 pKa = 4.97 FEE21 pKa = 5.65 ALWFPEE27 pKa = 3.68 RR28 pKa = 11.84 HH29 pKa = 5.32 TVDD32 pKa = 4.7 LSNGTCKK39 pKa = 9.99 LTGYY43 pKa = 8.13 ITNLPGYY50 pKa = 10.65 SDD52 pKa = 3.66 IFPNKK57 pKa = 9.32 GVTAARR63 pKa = 11.84 TPYY66 pKa = 10.4 RR67 pKa = 11.84 STVPVNHH74 pKa = 6.82 LGYY77 pKa = 10.5 RR78 pKa = 11.84 PVTTVEE84 pKa = 4.23 YY85 pKa = 10.01 IPDD88 pKa = 3.63 GTYY91 pKa = 10.56 VRR93 pKa = 11.84 LDD95 pKa = 3.21 GHH97 pKa = 6.06 VKK99 pKa = 10.68 FEE101 pKa = 4.2 GDD103 pKa = 3.64 LVNGSVDD110 pKa = 3.31 LTNFVISLAAQGGFDD125 pKa = 3.92 YY126 pKa = 11.11 QSVIGPRR133 pKa = 11.84 FSARR137 pKa = 11.84 FSAFSTKK144 pKa = 10.58 YY145 pKa = 10.48 GVLLGEE151 pKa = 4.26 GRR153 pKa = 11.84 EE154 pKa = 4.09 TLKK157 pKa = 11.12 YY158 pKa = 10.15 LLLVVRR164 pKa = 11.84 RR165 pKa = 11.84 MRR167 pKa = 11.84 EE168 pKa = 3.72 GYY170 pKa = 9.2 RR171 pKa = 11.84 AVRR174 pKa = 11.84 RR175 pKa = 11.84 GDD177 pKa = 3.48 LKK179 pKa = 10.75 RR180 pKa = 11.84 LRR182 pKa = 11.84 NVISTFEE189 pKa = 3.9 PSTIKK194 pKa = 10.57 GKK196 pKa = 9.62 RR197 pKa = 11.84 ARR199 pKa = 11.84 AEE201 pKa = 3.83 FSQTYY206 pKa = 9.68 RR207 pKa = 11.84 DD208 pKa = 3.74 KK209 pKa = 10.98 LTGNKK214 pKa = 9.44 VEE216 pKa = 4.12 VRR218 pKa = 11.84 PSEE221 pKa = 4.3 GKK223 pKa = 9.22 WNSSSASDD231 pKa = 3.0 LWLEE235 pKa = 3.89 FRR237 pKa = 11.84 YY238 pKa = 10.92 GLMPLFYY245 pKa = 10.26 DD246 pKa = 3.45 IQSVMEE252 pKa = 4.32 DD253 pKa = 3.16 FMRR256 pKa = 11.84 VHH258 pKa = 6.76 KK259 pKa = 10.46 KK260 pKa = 8.56 IAKK263 pKa = 8.24 IQRR266 pKa = 11.84 FSAGHH271 pKa = 6.04 GKK273 pKa = 10.43 LEE275 pKa = 4.44 TVSSRR280 pKa = 11.84 FYY282 pKa = 10.62 PDD284 pKa = 2.75 VHH286 pKa = 6.75 FSLEE290 pKa = 4.03 VTAVLQRR297 pKa = 11.84 RR298 pKa = 11.84 HH299 pKa = 5.06 RR300 pKa = 11.84 WGVIYY305 pKa = 10.45 QDD307 pKa = 3.34 TGSFATFNNGRR318 pKa = 11.84 LVPVKK323 pKa = 10.05 DD324 pKa = 3.07 WKK326 pKa = 9.34 TAAFALLNPAEE337 pKa = 4.61 VAWEE341 pKa = 3.92 VTPYY345 pKa = 11.07 SFVVDD350 pKa = 3.11 WFVNVGDD357 pKa = 4.51 MLEE360 pKa = 4.16 QMGQLYY366 pKa = 9.74 RR367 pKa = 11.84 HH368 pKa = 6.16 VDD370 pKa = 3.45 VVDD373 pKa = 3.84 GFDD376 pKa = 4.27 RR377 pKa = 11.84 KK378 pKa = 10.39 DD379 pKa = 3.13 IKK381 pKa = 10.88 LKK383 pKa = 9.86 SVSVRR388 pKa = 11.84 VLTNDD393 pKa = 3.15 VAHH396 pKa = 6.1 VASFQLRR403 pKa = 11.84 QAKK406 pKa = 9.53 LLHH409 pKa = 6.21 SYY411 pKa = 9.86 YY412 pKa = 10.93 SRR414 pKa = 11.84 VHH416 pKa = 5.56 TVAFPQISPQLDD428 pKa = 3.44 TEE430 pKa = 4.2 IRR432 pKa = 11.84 SVKK435 pKa = 10.36 HH436 pKa = 5.94 VIDD439 pKa = 4.9 SIALLTQRR447 pKa = 11.84 VKK449 pKa = 10.89 RR450 pKa = 3.95
Molecular weight: 51.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.887
IPC2_protein 9.194
IPC_protein 9.238
Toseland 9.692
ProMoST 9.56
Dawson 9.984
Bjellqvist 9.692
Wikipedia 10.189
Rodwell 10.218
Grimsley 10.087
Solomon 10.014
Lehninger 9.955
Nozaki 9.692
DTASelect 9.692
Thurlkill 9.809
EMBOSS 10.131
Sillero 9.897
Patrickios 5.219
IPC_peptide 9.999
IPC2_peptide 8.287
IPC2.peptide.svr19 8.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1489
132
576
372.3
41.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.455 ± 0.726
1.276 ± 0.386
6.985 ± 0.589
4.634 ± 0.516
4.365 ± 0.492
6.514 ± 0.376
1.41 ± 0.5
4.835 ± 0.682
4.835 ± 0.169
9.604 ± 0.449
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.209 ± 0.128
4.231 ± 0.481
5.037 ± 0.404
2.821 ± 0.326
7.79 ± 0.558
7.052 ± 0.368
6.38 ± 0.442
8.328 ± 0.941
1.545 ± 0.196
3.694 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here