Novosphingobium nitrogenifigens DSM 19370

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; Novosphingobium nitrogenifigens

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1Z8C4|F1Z8C4_9SPHN Uncharacterized protein OS=Novosphingobium nitrogenifigens DSM 19370 OX=983920 GN=Y88_1163 PE=4 SV=1
MM1 pKa = 7.21RR2 pKa = 11.84TTYY5 pKa = 11.07EE6 pKa = 3.99DD7 pKa = 3.22ATVRR11 pKa = 11.84LYY13 pKa = 11.03YY14 pKa = 10.79LSDD17 pKa = 3.75GPEE20 pKa = 3.96GGVAQTLMYY29 pKa = 10.62GPLSDD34 pKa = 5.14AMALAAQQEE43 pKa = 4.5PAVQDD48 pKa = 3.98GLWIATSNDD57 pKa = 2.77VVAWRR62 pKa = 11.84DD63 pKa = 3.38LAEE66 pKa = 4.19GG67 pKa = 3.64

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1ZDK0|F1ZDK0_9SPHN Uncharacterized protein OS=Novosphingobium nitrogenifigens DSM 19370 OX=983920 GN=Y88_3568 PE=4 SV=1
MM1 pKa = 7.38AACPRR6 pKa = 11.84FRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84TFSRR15 pKa = 11.84FGRR18 pKa = 11.84SRR20 pKa = 11.84LLLRR24 pKa = 11.84TGAHH28 pKa = 5.74RR29 pKa = 11.84RR30 pKa = 11.84KK31 pKa = 8.73TGGKK35 pKa = 8.97KK36 pKa = 9.53RR37 pKa = 11.84KK38 pKa = 9.08KK39 pKa = 10.36KK40 pKa = 10.5NGTSRR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 8.57MAFHH51 pKa = 6.75RR52 pKa = 11.84VSVVNSAATRR62 pKa = 11.84GLGG65 pKa = 3.33

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3800

0

3800

1232021

37

1713

324.2

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.327 ± 0.055

0.853 ± 0.012

5.805 ± 0.025

5.195 ± 0.035

3.515 ± 0.022

9.003 ± 0.036

2.299 ± 0.018

4.864 ± 0.028

2.678 ± 0.028

10.054 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.019

2.46 ± 0.025

5.464 ± 0.031

3.043 ± 0.024

7.363 ± 0.042

5.197 ± 0.031

5.447 ± 0.034

7.381 ± 0.03

1.439 ± 0.016

2.202 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski