Amino acid dipepetide frequency for Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.348AlaAla: 19.348 ± 0.133
1.086AlaCys: 1.086 ± 0.024
8.269AlaAsp: 8.269 ± 0.059
9.904AlaGlu: 9.904 ± 0.091
3.426AlaPhe: 3.426 ± 0.045
13.235AlaGly: 13.235 ± 0.077
2.716AlaHis: 2.716 ± 0.037
3.81AlaIle: 3.81 ± 0.052
2.394AlaLys: 2.394 ± 0.041
14.017AlaLeu: 14.017 ± 0.108
2.674AlaMet: 2.674 ± 0.042
2.033AlaAsn: 2.033 ± 0.032
6.389AlaPro: 6.389 ± 0.06
3.635AlaGln: 3.635 ± 0.045
9.891AlaArg: 9.891 ± 0.088
5.8AlaSer: 5.8 ± 0.054
6.537AlaThr: 6.537 ± 0.056
12.45AlaVal: 12.45 ± 0.105
1.718AlaTrp: 1.718 ± 0.029
2.138AlaTyr: 2.138 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
1.1CysAla: 1.1 ± 0.024
0.106CysCys: 0.106 ± 0.006
0.531CysAsp: 0.531 ± 0.014
0.478CysGlu: 0.478 ± 0.012
0.243CysPhe: 0.243 ± 0.011
1.01CysGly: 1.01 ± 0.024
0.198CysHis: 0.198 ± 0.009
0.151CysIle: 0.151 ± 0.007
0.115CysLys: 0.115 ± 0.008
0.731CysLeu: 0.731 ± 0.02
0.132CysMet: 0.132 ± 0.008
0.146CysAsn: 0.146 ± 0.008
0.481CysPro: 0.481 ± 0.016
0.191CysGln: 0.191 ± 0.01
0.679CysArg: 0.679 ± 0.02
0.506CysSer: 0.506 ± 0.016
0.481CysThr: 0.481 ± 0.014
0.679CysVal: 0.679 ± 0.019
0.146CysTrp: 0.146 ± 0.008
0.173CysTyr: 0.173 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.52AspAla: 7.52 ± 0.068
0.415AspCys: 0.415 ± 0.013
3.853AspAsp: 3.853 ± 0.048
4.324AspGlu: 4.324 ± 0.048
1.714AspPhe: 1.714 ± 0.027
6.017AspGly: 6.017 ± 0.066
1.492AspHis: 1.492 ± 0.029
1.77AspIle: 1.77 ± 0.031
0.943AspLys: 0.943 ± 0.025
6.195AspLeu: 6.195 ± 0.062
0.831AspMet: 0.831 ± 0.02
0.959AspAsn: 0.959 ± 0.022
4.384AspPro: 4.384 ± 0.05
1.764AspGln: 1.764 ± 0.029
5.049AspArg: 5.049 ± 0.059
2.702AspSer: 2.702 ± 0.043
2.721AspThr: 2.721 ± 0.033
5.296AspVal: 5.296 ± 0.056
0.952AspTrp: 0.952 ± 0.023
1.213AspTyr: 1.213 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.074GluAla: 7.074 ± 0.075
0.418GluCys: 0.418 ± 0.012
2.802GluAsp: 2.802 ± 0.037
3.356GluGlu: 3.356 ± 0.041
1.828GluPhe: 1.828 ± 0.032
4.058GluGly: 4.058 ± 0.047
2.022GluHis: 2.022 ± 0.028
2.389GluIle: 2.389 ± 0.036
1.179GluLys: 1.179 ± 0.026
7.957GluLeu: 7.957 ± 0.083
1.006GluMet: 1.006 ± 0.02
1.015GluAsn: 1.015 ± 0.022
3.875GluPro: 3.875 ± 0.045
2.834GluGln: 2.834 ± 0.04
5.931GluArg: 5.931 ± 0.058
3.071GluSer: 3.071 ± 0.043
2.788GluThr: 2.788 ± 0.037
5.53GluVal: 5.53 ± 0.051
0.873GluTrp: 0.873 ± 0.021
1.025GluTyr: 1.025 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.086PheAla: 4.086 ± 0.044
0.29PheCys: 0.29 ± 0.012
2.255PheAsp: 2.255 ± 0.032
1.551PheGlu: 1.551 ± 0.027
0.903PhePhe: 0.903 ± 0.021
3.435PheGly: 3.435 ± 0.048
0.67PheHis: 0.67 ± 0.017
0.697PheIle: 0.697 ± 0.019
0.373PheLys: 0.373 ± 0.012
2.598PheLeu: 2.598 ± 0.043
0.399PheMet: 0.399 ± 0.015
0.556PheAsn: 0.556 ± 0.017
1.381PhePro: 1.381 ± 0.023
0.685PheGln: 0.685 ± 0.019
1.902PheArg: 1.902 ± 0.03
1.525PheSer: 1.525 ± 0.028
1.783PheThr: 1.783 ± 0.032
2.555PheVal: 2.555 ± 0.033
0.426PheTrp: 0.426 ± 0.013
0.594PheTyr: 0.594 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
10.787GlyAla: 10.787 ± 0.077
0.885GlyCys: 0.885 ± 0.021
5.117GlyAsp: 5.117 ± 0.057
5.727GlyGlu: 5.727 ± 0.052
3.16GlyPhe: 3.16 ± 0.041
8.97GlyGly: 8.97 ± 0.092
2.255GlyHis: 2.255 ± 0.033
3.551GlyIle: 3.551 ± 0.045
2.088GlyLys: 2.088 ± 0.04
9.387GlyLeu: 9.387 ± 0.078
2.222GlyMet: 2.222 ± 0.034
1.716GlyAsn: 1.716 ± 0.035
4.767GlyPro: 4.767 ± 0.048
2.771GlyGln: 2.771 ± 0.044
7.725GlyArg: 7.725 ± 0.07
5.402GlySer: 5.402 ± 0.065
5.582GlyThr: 5.582 ± 0.055
8.327GlyVal: 8.327 ± 0.082
1.731GlyTrp: 1.731 ± 0.033
2.23GlyTyr: 2.23 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.754HisAla: 2.754 ± 0.037
0.253HisCys: 0.253 ± 0.012
1.511HisAsp: 1.511 ± 0.028
1.315HisGlu: 1.315 ± 0.025
0.67HisPhe: 0.67 ± 0.019
2.458HisGly: 2.458 ± 0.035
0.739HisHis: 0.739 ± 0.02
0.598HisIle: 0.598 ± 0.017
0.288HisLys: 0.288 ± 0.011
2.436HisLeu: 2.436 ± 0.036
0.328HisMet: 0.328 ± 0.012
0.423HisAsn: 0.423 ± 0.015
1.796HisPro: 1.796 ± 0.038
0.694HisGln: 0.694 ± 0.017
2.241HisArg: 2.241 ± 0.037
1.083HisSer: 1.083 ± 0.024
1.097HisThr: 1.097 ± 0.022
1.967HisVal: 1.967 ± 0.031
0.376HisTrp: 0.376 ± 0.014
0.526HisTyr: 0.526 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
5.043IleAla: 5.043 ± 0.047
0.279IleCys: 0.279 ± 0.011
2.342IleAsp: 2.342 ± 0.028
2.023IleGlu: 2.023 ± 0.033
0.667IlePhe: 0.667 ± 0.018
3.71IleGly: 3.71 ± 0.051
0.539IleHis: 0.539 ± 0.018
0.851IleIle: 0.851 ± 0.024
0.595IleLys: 0.595 ± 0.018
2.031IleLeu: 2.031 ± 0.036
0.446IleMet: 0.446 ± 0.015
0.734IleAsn: 0.734 ± 0.022
1.735IlePro: 1.735 ± 0.029
0.632IleGln: 0.632 ± 0.02
2.312IleArg: 2.312 ± 0.029
1.813IleSer: 1.813 ± 0.033
2.183IleThr: 2.183 ± 0.033
2.743IleVal: 2.743 ± 0.048
0.318IleTrp: 0.318 ± 0.012
0.558IleTyr: 0.558 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.183LysAla: 2.183 ± 0.039
0.103LysCys: 0.103 ± 0.006
0.881LysAsp: 0.881 ± 0.024
0.797LysGlu: 0.797 ± 0.02
0.424LysPhe: 0.424 ± 0.014
1.293LysGly: 1.293 ± 0.025
0.451LysHis: 0.451 ± 0.014
0.715LysIle: 0.715 ± 0.02
0.49LysLys: 0.49 ± 0.02
1.984LysLeu: 1.984 ± 0.032
0.314LysMet: 0.314 ± 0.01
0.356LysAsn: 0.356 ± 0.015
1.244LysPro: 1.244 ± 0.033
0.721LysGln: 0.721 ± 0.019
1.416LysArg: 1.416 ± 0.029
0.987LysSer: 0.987 ± 0.022
1.021LysThr: 1.021 ± 0.026
1.624LysVal: 1.624 ± 0.03
0.252LysTrp: 0.252 ± 0.011
0.333LysTyr: 0.333 ± 0.013
0.0LysXaa: 0.0 ± 0.0
Leu
15.04LeuAla: 15.04 ± 0.118
0.873LeuCys: 0.873 ± 0.021
6.793LeuAsp: 6.793 ± 0.056
5.174LeuGlu: 5.174 ± 0.053
2.739LeuPhe: 2.739 ± 0.042
9.576LeuGly: 9.576 ± 0.071
2.456LeuHis: 2.456 ± 0.034
2.899LeuIle: 2.899 ± 0.046
1.462LeuLys: 1.462 ± 0.028
11.308LeuLeu: 11.308 ± 0.112
1.523LeuMet: 1.523 ± 0.031
1.705LeuAsn: 1.705 ± 0.028
6.253LeuPro: 6.253 ± 0.061
2.107LeuGln: 2.107 ± 0.032
9.394LeuArg: 9.394 ± 0.082
5.735LeuSer: 5.735 ± 0.051
5.792LeuThr: 5.792 ± 0.061
9.869LeuVal: 9.869 ± 0.085
1.276LeuTrp: 1.276 ± 0.024
1.593LeuTyr: 1.593 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.332MetAla: 2.332 ± 0.034
0.157MetCys: 0.157 ± 0.008
0.844MetAsp: 0.844 ± 0.018
0.676MetGlu: 0.676 ± 0.019
0.525MetPhe: 0.525 ± 0.016
1.319MetGly: 1.319 ± 0.024
0.4MetHis: 0.4 ± 0.013
0.659MetIle: 0.659 ± 0.019
0.309MetLys: 0.309 ± 0.013
2.026MetLeu: 2.026 ± 0.031
0.296MetMet: 0.296 ± 0.012
0.378MetAsn: 0.378 ± 0.012
1.198MetPro: 1.198 ± 0.025
0.49MetGln: 0.49 ± 0.015
1.704MetArg: 1.704 ± 0.026
1.386MetSer: 1.386 ± 0.025
1.402MetThr: 1.402 ± 0.029
1.439MetVal: 1.439 ± 0.026
0.225MetTrp: 0.225 ± 0.01
0.286MetTyr: 0.286 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.263AsnAla: 2.263 ± 0.032
0.18AsnCys: 0.18 ± 0.008
0.931AsnAsp: 0.931 ± 0.023
0.812AsnGlu: 0.812 ± 0.019
0.512AsnPhe: 0.512 ± 0.013
1.78AsnGly: 1.78 ± 0.035
0.392AsnHis: 0.392 ± 0.013
0.632AsnIle: 0.632 ± 0.017
0.328AsnLys: 0.328 ± 0.014
1.747AsnLeu: 1.747 ± 0.028
0.295AsnMet: 0.295 ± 0.013
0.404AsnAsn: 0.404 ± 0.015
1.502AsnPro: 1.502 ± 0.031
0.527AsnGln: 0.527 ± 0.017
1.393AsnArg: 1.393 ± 0.025
0.97AsnSer: 0.97 ± 0.023
1.022AsnThr: 1.022 ± 0.023
1.364AsnVal: 1.364 ± 0.027
0.298AsnTrp: 0.298 ± 0.012
0.414AsnTyr: 0.414 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.011ProAla: 8.011 ± 0.075
0.37ProCys: 0.37 ± 0.016
4.386ProAsp: 4.386 ± 0.053
4.553ProGlu: 4.553 ± 0.045
1.531ProPhe: 1.531 ± 0.025
6.335ProGly: 6.335 ± 0.066
1.354ProHis: 1.354 ± 0.025
1.408ProIle: 1.408 ± 0.025
1.093ProLys: 1.093 ± 0.026
5.009ProLeu: 5.009 ± 0.055
1.065ProMet: 1.065 ± 0.024
1.033ProAsn: 1.033 ± 0.023
3.523ProPro: 3.523 ± 0.069
1.835ProGln: 1.835 ± 0.036
4.045ProArg: 4.045 ± 0.054
3.136ProSer: 3.136 ± 0.04
2.945ProThr: 2.945 ± 0.04
5.536ProVal: 5.536 ± 0.048
0.908ProTrp: 0.908 ± 0.021
1.048ProTyr: 1.048 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.847GlnAla: 3.847 ± 0.046
0.201GlnCys: 0.201 ± 0.01
1.378GlnAsp: 1.378 ± 0.027
1.477GlnGlu: 1.477 ± 0.029
0.699GlnPhe: 0.699 ± 0.02
2.167GlnGly: 2.167 ± 0.03
0.753GlnHis: 0.753 ± 0.018
1.047GlnIle: 1.047 ± 0.02
0.465GlnLys: 0.465 ± 0.015
2.929GlnLeu: 2.929 ± 0.042
0.47GlnMet: 0.47 ± 0.015
0.501GlnAsn: 0.501 ± 0.017
1.951GlnPro: 1.951 ± 0.042
1.467GlnGln: 1.467 ± 0.037
3.044GlnArg: 3.044 ± 0.032
1.237GlnSer: 1.237 ± 0.027
1.255GlnThr: 1.255 ± 0.031
2.728GlnVal: 2.728 ± 0.038
0.49GlnTrp: 0.49 ± 0.015
0.566GlnTyr: 0.566 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
9.813ArgAla: 9.813 ± 0.09
0.679ArgCys: 0.679 ± 0.02
4.548ArgAsp: 4.548 ± 0.049
5.372ArgGlu: 5.372 ± 0.063
2.614ArgPhe: 2.614 ± 0.035
6.407ArgGly: 6.407 ± 0.066
2.101ArgHis: 2.101 ± 0.029
3.311ArgIle: 3.311 ± 0.045
1.681ArgLys: 1.681 ± 0.034
8.787ArgLeu: 8.787 ± 0.086
2.04ArgMet: 2.04 ± 0.034
1.522ArgAsn: 1.522 ± 0.028
4.814ArgPro: 4.814 ± 0.051
2.483ArgGln: 2.483 ± 0.033
8.463ArgArg: 8.463 ± 0.098
4.548ArgSer: 4.548 ± 0.048
4.999ArgThr: 4.999 ± 0.052
6.347ArgVal: 6.347 ± 0.058
1.57ArgTrp: 1.57 ± 0.031
1.843ArgTyr: 1.843 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
7.172SerAla: 7.172 ± 0.057
0.444SerCys: 0.444 ± 0.013
2.865SerAsp: 2.865 ± 0.036
2.876SerGlu: 2.876 ± 0.046
1.602SerPhe: 1.602 ± 0.027
6.089SerGly: 6.089 ± 0.07
1.013SerHis: 1.013 ± 0.02
1.582SerIle: 1.582 ± 0.03
0.98SerLys: 0.98 ± 0.023
4.753SerLeu: 4.753 ± 0.046
1.197SerMet: 1.197 ± 0.023
0.93SerAsn: 0.93 ± 0.018
3.126SerPro: 3.126 ± 0.037
1.338SerGln: 1.338 ± 0.027
4.056SerArg: 4.056 ± 0.044
3.059SerSer: 3.059 ± 0.049
3.193SerThr: 3.193 ± 0.046
4.494SerVal: 4.494 ± 0.045
0.967SerTrp: 0.967 ± 0.023
1.091SerTyr: 1.091 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
7.861ThrAla: 7.861 ± 0.067
0.445ThrCys: 0.445 ± 0.016
3.222ThrAsp: 3.222 ± 0.035
3.274ThrGlu: 3.274 ± 0.034
1.517ThrPhe: 1.517 ± 0.028
6.222ThrGly: 6.222 ± 0.059
1.056ThrHis: 1.056 ± 0.024
1.76ThrIle: 1.76 ± 0.035
0.908ThrLys: 0.908 ± 0.023
4.835ThrLeu: 4.835 ± 0.055
0.938ThrMet: 0.938 ± 0.023
0.959ThrAsn: 0.959 ± 0.022
3.501ThrPro: 3.501 ± 0.041
1.154ThrGln: 1.154 ± 0.025
3.789ThrArg: 3.789 ± 0.039
3.143ThrSer: 3.143 ± 0.036
3.542ThrThr: 3.542 ± 0.05
4.98ThrVal: 4.98 ± 0.056
0.871ThrTrp: 0.871 ± 0.019
1.107ThrTyr: 1.107 ± 0.021
0.0ThrXaa: 0.0 ± 0.0
Val
11.481ValAla: 11.481 ± 0.081
0.76ValCys: 0.76 ± 0.017
5.625ValAsp: 5.625 ± 0.06
5.571ValGlu: 5.571 ± 0.052
2.663ValPhe: 2.663 ± 0.041
7.319ValGly: 7.319 ± 0.059
2.142ValHis: 2.142 ± 0.034
2.77ValIle: 2.77 ± 0.037
1.311ValLys: 1.311 ± 0.029
10.828ValLeu: 10.828 ± 0.092
1.318ValMet: 1.318 ± 0.024
1.647ValAsn: 1.647 ± 0.03
5.273ValPro: 5.273 ± 0.052
2.13ValGln: 2.13 ± 0.028
7.651ValArg: 7.651 ± 0.066
4.722ValSer: 4.722 ± 0.051
4.866ValThr: 4.866 ± 0.044
9.587ValVal: 9.587 ± 0.09
1.121ValTrp: 1.121 ± 0.022
1.411ValTyr: 1.411 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.544TrpAla: 1.544 ± 0.031
0.151TrpCys: 0.151 ± 0.008
0.749TrpAsp: 0.749 ± 0.019
0.763TrpGlu: 0.763 ± 0.021
0.565TrpPhe: 0.565 ± 0.016
1.05TrpGly: 1.05 ± 0.024
0.383TrpHis: 0.383 ± 0.014
0.546TrpIle: 0.546 ± 0.017
0.318TrpLys: 0.318 ± 0.012
1.914TrpLeu: 1.914 ± 0.034
0.349TrpMet: 0.349 ± 0.012
0.373TrpAsn: 0.373 ± 0.014
0.853TrpPro: 0.853 ± 0.02
0.594TrpGln: 0.594 ± 0.018
1.397TrpArg: 1.397 ± 0.025
1.002TrpSer: 1.002 ± 0.019
0.898TrpThr: 0.898 ± 0.02
1.104TrpVal: 1.104 ± 0.024
0.363TrpTrp: 0.363 ± 0.014
0.304TrpTyr: 0.304 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.214TyrAla: 2.214 ± 0.034
0.18TyrCys: 0.18 ± 0.009
1.21TyrAsp: 1.21 ± 0.028
1.005TyrGlu: 1.005 ± 0.022
0.663TyrPhe: 0.663 ± 0.02
1.947TyrGly: 1.947 ± 0.035
0.423TyrHis: 0.423 ± 0.012
0.412TyrIle: 0.412 ± 0.014
0.271TyrLys: 0.271 ± 0.01
2.097TyrLeu: 2.097 ± 0.033
0.21TyrMet: 0.21 ± 0.01
0.376TyrAsn: 0.376 ± 0.014
1.075TyrPro: 1.075 ± 0.019
0.621TyrGln: 0.621 ± 0.016
1.861TyrArg: 1.861 ± 0.027
0.998TyrSer: 0.998 ± 0.02
0.979TyrThr: 0.979 ± 0.019
1.576TyrVal: 1.576 ± 0.025
0.336TyrTrp: 0.336 ± 0.013
0.429TyrTyr: 0.429 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7154 proteins (2307965 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski