Deep-sea thermophilic phage D6E

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5DV61|E5DV61_9CAUD Uncharacterized protein OS=Deep-sea thermophilic phage D6E OX=749413 PE=4 SV=1
MM1 pKa = 7.54HH2 pKa = 7.52IIEE5 pKa = 4.53IDD7 pKa = 3.57KK8 pKa = 11.36NNLPEE13 pKa = 3.92QFNIEE18 pKa = 4.31LGTEE22 pKa = 4.63LFTMEE27 pKa = 3.97VNYY30 pKa = 11.13NEE32 pKa = 4.33TGDD35 pKa = 3.89FFTIDD40 pKa = 4.05LYY42 pKa = 11.27QYY44 pKa = 10.76DD45 pKa = 4.13NPEE48 pKa = 4.12PLVLGEE54 pKa = 4.29KK55 pKa = 10.47LMYY58 pKa = 9.53GQPLFQDD65 pKa = 3.5IADD68 pKa = 3.97SRR70 pKa = 11.84FPAPTIVPLDD80 pKa = 3.71LSGKK84 pKa = 5.66EE85 pKa = 4.33TRR87 pKa = 11.84VSWDD91 pKa = 3.15NFGVTVFLVIDD102 pKa = 4.41DD103 pKa = 4.58GEE105 pKa = 3.97

Molecular weight: 12.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5DV82|E5DV82_9CAUD Replication protein OS=Deep-sea thermophilic phage D6E OX=749413 PE=4 SV=1
MM1 pKa = 7.67SGRR4 pKa = 11.84KK5 pKa = 9.2KK6 pKa = 10.22PVEE9 pKa = 3.5KK10 pKa = 10.1AYY12 pKa = 10.72RR13 pKa = 11.84PGLEE17 pKa = 4.02YY18 pKa = 10.55EE19 pKa = 4.4VAIPHH24 pKa = 6.67CYY26 pKa = 9.73KK27 pKa = 10.22WMARR31 pKa = 11.84TKK33 pKa = 10.13QEE35 pKa = 3.94YY36 pKa = 8.47IGYY39 pKa = 9.2VMGYY43 pKa = 9.27VRR45 pKa = 11.84ASHH48 pKa = 7.0PGFKK52 pKa = 10.17VIRR55 pKa = 11.84IEE57 pKa = 3.95KK58 pKa = 10.08GKK60 pKa = 10.4AICIKK65 pKa = 10.16EE66 pKa = 4.15DD67 pKa = 3.49KK68 pKa = 10.77QSGG71 pKa = 3.52

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10731

45

752

219.0

24.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.315 ± 0.377

0.699 ± 0.117

6.272 ± 0.32

7.912 ± 0.453

3.737 ± 0.229

6.449 ± 0.252

1.752 ± 0.183

6.719 ± 0.317

7.912 ± 0.384

8.163 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.647 ± 0.13

4.594 ± 0.192

3.485 ± 0.206

4.119 ± 0.271

5.759 ± 0.331

4.753 ± 0.256

5.778 ± 0.408

6.877 ± 0.381

1.221 ± 0.114

3.839 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski