Clostridium saccharoperbutylacetonicum N1-4(HMT)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium saccharoperbutylacetonicum; Clostridium saccharoperbutylacetonicum N1-4

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5810 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1LYH0|M1LYH0_9CLOT Lipopolysaccharide biosynthesis protein OS=Clostridium saccharoperbutylacetonicum N1-4(HMT) OX=931276 GN=Cspa_c45520 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.11SSEE5 pKa = 4.07IIEE8 pKa = 4.11YY9 pKa = 11.06LEE11 pKa = 5.19DD12 pKa = 3.8NDD14 pKa = 4.39LADD17 pKa = 4.35VEE19 pKa = 4.69EE20 pKa = 4.8IKK22 pKa = 10.85QKK24 pKa = 10.82SNYY27 pKa = 9.83VIIKK31 pKa = 9.57FYY33 pKa = 11.16YY34 pKa = 10.61DD35 pKa = 3.47FDD37 pKa = 4.17NEE39 pKa = 4.31EE40 pKa = 3.57ISAAKK45 pKa = 10.06AYY47 pKa = 8.97STEE50 pKa = 3.96EE51 pKa = 3.87SDD53 pKa = 5.79FEE55 pKa = 4.51PEE57 pKa = 3.21TDD59 pKa = 2.72EE60 pKa = 4.94WYY62 pKa = 10.27NEE64 pKa = 4.1CYY66 pKa = 10.01IPYY69 pKa = 9.99LRR71 pKa = 11.84DD72 pKa = 3.12IAVDD76 pKa = 3.6NVEE79 pKa = 4.65SIIDD83 pKa = 4.15EE84 pKa = 4.23ISDD87 pKa = 3.4EE88 pKa = 4.43LEE90 pKa = 3.98IEE92 pKa = 4.32GKK94 pKa = 10.2YY95 pKa = 10.2KK96 pKa = 10.38EE97 pKa = 4.25FGMEE101 pKa = 3.9NVEE104 pKa = 3.61NGYY107 pKa = 10.77YY108 pKa = 10.47KK109 pKa = 10.69FIAVFSDD116 pKa = 3.23EE117 pKa = 4.31LTDD120 pKa = 4.56EE121 pKa = 4.18EE122 pKa = 5.02MEE124 pKa = 4.84DD125 pKa = 4.39VLNDD129 pKa = 3.23YY130 pKa = 10.96HH131 pKa = 7.38KK132 pKa = 11.27

Molecular weight: 15.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1M9L6|M1M9L6_9CLOT Glutamine transport ATP-binding protein GlnQ OS=Clostridium saccharoperbutylacetonicum N1-4(HMT) OX=931276 GN=glnQ1 PE=4 SV=1
MM1 pKa = 7.04NRR3 pKa = 11.84KK4 pKa = 8.49NNRR7 pKa = 11.84VIGAVSHH14 pKa = 6.54IVFPLATCVFLVWGFLFHH32 pKa = 6.88GWRR35 pKa = 11.84ICWIVYY41 pKa = 8.79PITGILFSAFARR53 pKa = 11.84TYY55 pKa = 11.46SLMNGANKK63 pKa = 10.25

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5810

0

5810

1832897

29

3754

315.5

35.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.827 ± 0.038

1.205 ± 0.013

5.606 ± 0.024

7.39 ± 0.042

4.397 ± 0.023

6.363 ± 0.038

1.362 ± 0.013

9.762 ± 0.043

8.844 ± 0.032

8.848 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.578 ± 0.014

6.647 ± 0.031

2.742 ± 0.018

2.491 ± 0.019

3.098 ± 0.019

6.416 ± 0.029

5.091 ± 0.038

6.226 ± 0.026

0.821 ± 0.01

4.284 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski