Streptococcus phage Str-PAP-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5A2G1|A0A0B5A2G1_9CAUD Endonuclease OS=Streptococcus phage Str-PAP-1 OX=1589270 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.4LYY4 pKa = 10.2ILEE7 pKa = 4.17FFKK10 pKa = 10.83FDD12 pKa = 3.67YY13 pKa = 10.03EE14 pKa = 4.2CSYY17 pKa = 9.7THH19 pKa = 7.23ILGVYY24 pKa = 10.29DD25 pKa = 3.94NLDD28 pKa = 3.57DD29 pKa = 4.02ARR31 pKa = 11.84EE32 pKa = 4.45FIDD35 pKa = 3.46SKK37 pKa = 10.94GYY39 pKa = 10.23RR40 pKa = 11.84YY41 pKa = 9.88CKK43 pKa = 10.42KK44 pKa = 10.42IFDD47 pKa = 5.3DD48 pKa = 5.92DD49 pKa = 4.12LEE51 pKa = 4.43QYY53 pKa = 10.01EE54 pKa = 4.56KK55 pKa = 10.82EE56 pKa = 4.37DD57 pKa = 3.34RR58 pKa = 11.84TITIIEE64 pKa = 4.02RR65 pKa = 11.84DD66 pKa = 3.72LNKK69 pKa = 10.51DD70 pKa = 3.38CSISSVEE77 pKa = 4.06GG78 pKa = 3.31

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5A550|A0A0B5A550_9CAUD Structural protein OS=Streptococcus phage Str-PAP-1 OX=1589270 PE=4 SV=1
MM1 pKa = 6.76NWKK4 pKa = 9.98IIEE7 pKa = 4.05EE8 pKa = 4.11RR9 pKa = 11.84NNYY12 pKa = 9.63SINEE16 pKa = 4.01NGEE19 pKa = 3.54IRR21 pKa = 11.84NDD23 pKa = 2.96KK24 pKa = 9.68TGRR27 pKa = 11.84ILKK30 pKa = 10.06LRR32 pKa = 11.84VGTSGYY38 pKa = 7.9EE39 pKa = 3.73QIMLGRR45 pKa = 11.84KK46 pKa = 4.82TTPLYY51 pKa = 9.48VHH53 pKa = 7.06RR54 pKa = 11.84LVAKK58 pKa = 10.6AFIKK62 pKa = 9.09NTKK65 pKa = 9.58NLPQVDD71 pKa = 4.85HH72 pKa = 7.01INGIKK77 pKa = 9.16TDD79 pKa = 3.41NRR81 pKa = 11.84ADD83 pKa = 3.46NLRR86 pKa = 11.84WVTVSEE92 pKa = 4.28NCWGYY97 pKa = 10.91GYY99 pKa = 10.52EE100 pKa = 3.95GRR102 pKa = 11.84VKK104 pKa = 10.53NRR106 pKa = 11.84QKK108 pKa = 10.7KK109 pKa = 9.01ISAFNEE115 pKa = 4.25TLDD118 pKa = 3.32KK119 pKa = 11.0TIIFNSRR126 pKa = 11.84NEE128 pKa = 3.88TAKK131 pKa = 10.51FFNTHH136 pKa = 5.94KK137 pKa = 11.01SQIKK141 pKa = 9.94YY142 pKa = 9.52NYY144 pKa = 9.04EE145 pKa = 3.78YY146 pKa = 11.21KK147 pKa = 10.57KK148 pKa = 10.8GNKK151 pKa = 9.2KK152 pKa = 9.84GWIFNLVKK160 pKa = 10.77DD161 pKa = 3.94IVV163 pKa = 3.42

Molecular weight: 19.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11055

38

987

193.9

22.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.807 ± 0.384

0.832 ± 0.195

6.214 ± 0.259

6.974 ± 0.356

4.052 ± 0.27

6.712 ± 0.328

1.601 ± 0.19

7.273 ± 0.365

8.82 ± 0.469

8.033 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.161

6.603 ± 0.347

2.976 ± 0.196

3.528 ± 0.222

3.953 ± 0.274

6.395 ± 0.318

6.178 ± 0.372

6.097 ± 0.245

1.429 ± 0.16

4.261 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski