Legionella clemsonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2917 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222P1X6|A0A222P1X6_9GAMM Ancillary SecYEG translocon subunit OS=Legionella clemsonensis OX=1867846 GN=clem_06440 PE=3 SV=1
MM1 pKa = 7.42AAIDD5 pKa = 3.79VSPSVSSIHH14 pKa = 6.1FSVSAADD21 pKa = 4.1KK22 pKa = 9.87VASLIAEE29 pKa = 4.14EE30 pKa = 4.59EE31 pKa = 4.21NPNLNLRR38 pKa = 11.84VFVTGGGCSGLQYY51 pKa = 11.19GFTFDD56 pKa = 3.51EE57 pKa = 5.39TIQEE61 pKa = 4.51DD62 pKa = 3.84DD63 pKa = 4.08TIIEE67 pKa = 4.37QTCSDD72 pKa = 3.64GKK74 pKa = 11.15SSVKK78 pKa = 10.59LLVDD82 pKa = 3.58SMSYY86 pKa = 10.52QYY88 pKa = 11.62LNAAEE93 pKa = 4.24IDD95 pKa = 3.99YY96 pKa = 10.27VQNIQGAQFVIRR108 pKa = 11.84NPNAKK113 pKa = 6.55TTCGCGSSFSMDD125 pKa = 3.56DD126 pKa = 3.98DD127 pKa = 4.02EE128 pKa = 5.54

Molecular weight: 13.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222NYH4|A0A222NYH4_9GAMM Uncharacterized protein OS=Legionella clemsonensis OX=1867846 GN=clem_00310 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.18RR12 pKa = 11.84KK13 pKa = 8.83RR14 pKa = 11.84DD15 pKa = 3.29HH16 pKa = 7.0GFRR19 pKa = 11.84QRR21 pKa = 11.84MSTRR25 pKa = 11.84GGRR28 pKa = 11.84LVLKK32 pKa = 10.32RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2917

0

2917

962275

29

4735

329.9

37.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.083 ± 0.042

1.178 ± 0.017

4.534 ± 0.029

6.251 ± 0.053

4.513 ± 0.032

5.809 ± 0.046

2.534 ± 0.022

7.239 ± 0.039

6.221 ± 0.055

11.362 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.02

4.735 ± 0.039

4.115 ± 0.026

4.701 ± 0.034

4.433 ± 0.03

6.259 ± 0.036

5.357 ± 0.027

5.88 ± 0.042

1.137 ± 0.017

3.389 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski