Bryocella elongata

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Bryocella

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5ZFJ5|A0A1H5ZFJ5_9BACT Nucleobase:cation symporter-1 NCS1 family OS=Bryocella elongata OX=863522 GN=SAMN05421819_2636 PE=3 SV=1
MM1 pKa = 7.45TPQEE5 pKa = 3.92QQLIDD10 pKa = 3.71GLISRR15 pKa = 11.84IRR17 pKa = 11.84STPATDD23 pKa = 3.88KK24 pKa = 11.44DD25 pKa = 3.55VDD27 pKa = 3.54ADD29 pKa = 4.25KK30 pKa = 11.25YY31 pKa = 10.94LHH33 pKa = 6.34QNLDD37 pKa = 3.58SVPDD41 pKa = 3.62VLYY44 pKa = 11.26VLAQTVLVQQYY55 pKa = 10.07GLQNAQQQLQAAQQQADD72 pKa = 4.04DD73 pKa = 4.68LQAQLDD79 pKa = 3.79QARR82 pKa = 11.84AQAQQAQQSQKK93 pKa = 9.64SGSFLGHH100 pKa = 7.46LFGTDD105 pKa = 3.01NPQPQQGYY113 pKa = 7.61PQQGYY118 pKa = 7.65PQGYY122 pKa = 7.82PPQGYY127 pKa = 8.65AQPGYY132 pKa = 8.6VQAVPVQPMGYY143 pKa = 10.67GMGGGMGSPIGGGGFLRR160 pKa = 11.84GALQTAAGVAAGEE173 pKa = 4.33MVFQGMEE180 pKa = 3.72DD181 pKa = 3.3MFRR184 pKa = 11.84GFGHH188 pKa = 6.77EE189 pKa = 4.01GGGYY193 pKa = 9.42GGSRR197 pKa = 11.84PTEE200 pKa = 3.97VINNNYY206 pKa = 9.75YY207 pKa = 10.91DD208 pKa = 4.19DD209 pKa = 5.0DD210 pKa = 4.84RR211 pKa = 11.84PEE213 pKa = 4.47SGHH216 pKa = 5.17EE217 pKa = 3.77HH218 pKa = 6.24HH219 pKa = 7.44LEE221 pKa = 4.3GNDD224 pKa = 3.95DD225 pKa = 3.73SSFYY229 pKa = 11.02NAANDD234 pKa = 3.91ASSKK238 pKa = 10.75DD239 pKa = 3.77LSPDD243 pKa = 2.96IEE245 pKa = 4.32DD246 pKa = 3.4RR247 pKa = 11.84RR248 pKa = 11.84GFADD252 pKa = 3.83TGNNDD257 pKa = 4.07ANDD260 pKa = 4.21DD261 pKa = 3.99SQQGFDD267 pKa = 4.91DD268 pKa = 4.54NSSSDD273 pKa = 3.73DD274 pKa = 3.59SGFDD278 pKa = 4.58DD279 pKa = 5.16SSSDD283 pKa = 4.29DD284 pKa = 3.36GGGYY288 pKa = 10.26DD289 pKa = 5.06DD290 pKa = 5.39SSNN293 pKa = 3.22

Molecular weight: 31.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5WUH4|A0A1H5WUH4_9BACT Uncharacterized protein OS=Bryocella elongata OX=863522 GN=SAMN05421819_1730 PE=4 SV=1
MM1 pKa = 6.72TRR3 pKa = 11.84KK4 pKa = 9.59SIKK7 pKa = 8.64IHH9 pKa = 6.27KK10 pKa = 7.38PAPTLHH16 pKa = 6.66LPAHH20 pKa = 5.77EE21 pKa = 4.01QRR23 pKa = 11.84AAARR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84HH30 pKa = 5.6NAALPNIPGPKK41 pKa = 9.02PRR43 pKa = 11.84IARR46 pKa = 11.84AAKK49 pKa = 8.89PRR51 pKa = 11.84PIPAHH56 pKa = 6.44KK57 pKa = 9.4VTTPRR62 pKa = 11.84SVHH65 pKa = 5.53

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4503

0

4503

1673813

25

3776

371.7

40.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.471 ± 0.051

0.857 ± 0.011

5.116 ± 0.031

5.317 ± 0.048

3.788 ± 0.02

8.146 ± 0.046

2.327 ± 0.017

4.703 ± 0.027

3.417 ± 0.03

9.895 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.301 ± 0.018

3.259 ± 0.033

5.477 ± 0.029

3.677 ± 0.026

6.072 ± 0.04

6.464 ± 0.042

6.346 ± 0.056

7.246 ± 0.03

1.384 ± 0.018

2.738 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski