Amino acid dipepetide frequency for Mycobacterium phage Magnar

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.742AlaAla: 12.742 ± 1.089
0.439AlaCys: 0.439 ± 0.159
7.219AlaAsp: 7.219 ± 0.698
6.654AlaGlu: 6.654 ± 0.655
2.825AlaPhe: 2.825 ± 0.406
7.784AlaGly: 7.784 ± 0.771
1.381AlaHis: 1.381 ± 0.346
4.206AlaIle: 4.206 ± 0.552
4.268AlaLys: 4.268 ± 0.618
8.788AlaLeu: 8.788 ± 0.854
2.699AlaMet: 2.699 ± 0.507
2.197AlaAsn: 2.197 ± 0.396
4.959AlaPro: 4.959 ± 0.825
2.887AlaGln: 2.887 ± 0.406
5.9AlaArg: 5.9 ± 0.675
5.587AlaSer: 5.587 ± 0.77
5.524AlaThr: 5.524 ± 0.735
8.411AlaVal: 8.411 ± 0.686
1.82AlaTrp: 1.82 ± 0.386
2.762AlaTyr: 2.762 ± 0.457
0.0AlaXaa: 0.0 ± 0.0
Cys
0.753CysAla: 0.753 ± 0.228
0.0CysCys: 0.0 ± 0.0
0.377CysAsp: 0.377 ± 0.156
0.628CysGlu: 0.628 ± 0.222
0.188CysPhe: 0.188 ± 0.092
0.502CysGly: 0.502 ± 0.203
0.126CysHis: 0.126 ± 0.095
0.126CysIle: 0.126 ± 0.096
0.126CysLys: 0.126 ± 0.079
0.251CysLeu: 0.251 ± 0.127
0.126CysMet: 0.126 ± 0.069
0.188CysAsn: 0.188 ± 0.12
0.314CysPro: 0.314 ± 0.144
0.314CysGln: 0.314 ± 0.134
0.502CysArg: 0.502 ± 0.169
0.251CysSer: 0.251 ± 0.14
0.314CysThr: 0.314 ± 0.132
0.126CysVal: 0.126 ± 0.094
0.188CysTrp: 0.188 ± 0.102
0.126CysTyr: 0.126 ± 0.084
0.0CysXaa: 0.0 ± 0.0
Asp
6.716AspAla: 6.716 ± 0.721
0.439AspCys: 0.439 ± 0.153
4.959AspAsp: 4.959 ± 0.523
3.892AspGlu: 3.892 ± 0.423
2.574AspPhe: 2.574 ± 0.342
6.528AspGly: 6.528 ± 0.493
1.004AspHis: 1.004 ± 0.289
2.574AspIle: 2.574 ± 0.428
2.636AspLys: 2.636 ± 0.483
6.905AspLeu: 6.905 ± 0.776
1.13AspMet: 1.13 ± 0.237
1.946AspAsn: 1.946 ± 0.336
5.022AspPro: 5.022 ± 0.729
1.569AspGln: 1.569 ± 0.319
3.578AspArg: 3.578 ± 0.439
3.39AspSer: 3.39 ± 0.517
3.892AspThr: 3.892 ± 0.437
4.959AspVal: 4.959 ± 0.615
1.82AspTrp: 1.82 ± 0.341
2.134AspTyr: 2.134 ± 0.441
0.0AspXaa: 0.0 ± 0.0
Glu
6.026GluAla: 6.026 ± 0.611
0.188GluCys: 0.188 ± 0.13
5.273GluAsp: 5.273 ± 0.518
4.771GluGlu: 4.771 ± 0.598
2.26GluPhe: 2.26 ± 0.337
3.703GluGly: 3.703 ± 0.46
1.255GluHis: 1.255 ± 0.256
3.515GluIle: 3.515 ± 0.57
2.825GluLys: 2.825 ± 0.445
6.277GluLeu: 6.277 ± 0.583
1.758GluMet: 1.758 ± 0.315
1.569GluAsn: 1.569 ± 0.332
2.636GluPro: 2.636 ± 0.369
2.636GluGln: 2.636 ± 0.394
3.578GluArg: 3.578 ± 0.505
3.452GluSer: 3.452 ± 0.375
3.578GluThr: 3.578 ± 0.496
5.461GluVal: 5.461 ± 0.675
1.506GluTrp: 1.506 ± 0.296
2.071GluTyr: 2.071 ± 0.466
0.0GluXaa: 0.0 ± 0.0
Phe
2.511PheAla: 2.511 ± 0.349
0.377PheCys: 0.377 ± 0.169
2.762PheAsp: 2.762 ± 0.297
2.071PheGlu: 2.071 ± 0.335
0.628PhePhe: 0.628 ± 0.176
3.578PheGly: 3.578 ± 0.453
0.816PheHis: 0.816 ± 0.241
1.381PheIle: 1.381 ± 0.293
1.506PheLys: 1.506 ± 0.27
2.574PheLeu: 2.574 ± 0.484
0.439PheMet: 0.439 ± 0.128
1.067PheAsn: 1.067 ± 0.23
1.569PhePro: 1.569 ± 0.289
1.004PheGln: 1.004 ± 0.265
2.134PheArg: 2.134 ± 0.407
1.569PheSer: 1.569 ± 0.283
2.385PheThr: 2.385 ± 0.43
2.197PheVal: 2.197 ± 0.382
0.502PheTrp: 0.502 ± 0.159
0.942PheTyr: 0.942 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
7.846GlyAla: 7.846 ± 1.096
0.439GlyCys: 0.439 ± 0.155
5.587GlyAsp: 5.587 ± 0.471
4.206GlyGlu: 4.206 ± 0.53
3.139GlyPhe: 3.139 ± 0.48
9.855GlyGly: 9.855 ± 2.769
1.946GlyHis: 1.946 ± 0.349
4.582GlyIle: 4.582 ± 0.636
3.264GlyLys: 3.264 ± 0.449
7.909GlyLeu: 7.909 ± 0.866
1.883GlyMet: 1.883 ± 0.359
3.139GlyAsn: 3.139 ± 0.394
3.766GlyPro: 3.766 ± 0.537
2.071GlyGln: 2.071 ± 0.352
5.336GlyArg: 5.336 ± 0.472
6.34GlySer: 6.34 ± 0.766
4.582GlyThr: 4.582 ± 0.612
5.461GlyVal: 5.461 ± 0.568
2.636GlyTrp: 2.636 ± 0.361
2.825GlyTyr: 2.825 ± 0.369
0.0GlyXaa: 0.0 ± 0.0
His
1.758HisAla: 1.758 ± 0.367
0.188HisCys: 0.188 ± 0.144
1.13HisAsp: 1.13 ± 0.281
1.695HisGlu: 1.695 ± 0.328
0.69HisPhe: 0.69 ± 0.188
1.82HisGly: 1.82 ± 0.392
0.565HisHis: 0.565 ± 0.2
0.879HisIle: 0.879 ± 0.22
0.942HisLys: 0.942 ± 0.278
1.632HisLeu: 1.632 ± 0.319
0.251HisMet: 0.251 ± 0.146
0.251HisAsn: 0.251 ± 0.131
1.255HisPro: 1.255 ± 0.24
0.816HisGln: 0.816 ± 0.211
1.506HisArg: 1.506 ± 0.308
0.565HisSer: 0.565 ± 0.189
0.879HisThr: 0.879 ± 0.263
1.82HisVal: 1.82 ± 0.355
0.565HisTrp: 0.565 ± 0.141
0.565HisTyr: 0.565 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
6.152IleAla: 6.152 ± 0.597
0.314IleCys: 0.314 ± 0.124
3.515IleAsp: 3.515 ± 0.42
3.641IleGlu: 3.641 ± 0.434
0.753IlePhe: 0.753 ± 0.203
4.08IleGly: 4.08 ± 0.535
0.816IleHis: 0.816 ± 0.19
2.134IleIle: 2.134 ± 0.331
1.82IleLys: 1.82 ± 0.337
3.201IleLeu: 3.201 ± 0.381
0.942IleMet: 0.942 ± 0.232
1.82IleAsn: 1.82 ± 0.357
3.264IlePro: 3.264 ± 0.433
1.506IleGln: 1.506 ± 0.326
3.578IleArg: 3.578 ± 0.436
3.39IleSer: 3.39 ± 0.579
3.515IleThr: 3.515 ± 0.527
2.636IleVal: 2.636 ± 0.467
0.628IleTrp: 0.628 ± 0.177
1.569IleTyr: 1.569 ± 0.283
0.0IleXaa: 0.0 ± 0.0
Lys
3.578LysAla: 3.578 ± 0.567
0.314LysCys: 0.314 ± 0.138
2.511LysAsp: 2.511 ± 0.493
1.82LysGlu: 1.82 ± 0.332
1.444LysPhe: 1.444 ± 0.292
2.574LysGly: 2.574 ± 0.439
1.444LysHis: 1.444 ± 0.356
2.26LysIle: 2.26 ± 0.379
2.071LysLys: 2.071 ± 0.425
3.515LysLeu: 3.515 ± 0.412
0.879LysMet: 0.879 ± 0.228
1.381LysAsn: 1.381 ± 0.335
2.511LysPro: 2.511 ± 0.427
1.632LysGln: 1.632 ± 0.296
2.887LysArg: 2.887 ± 0.454
2.448LysSer: 2.448 ± 0.404
2.323LysThr: 2.323 ± 0.38
2.95LysVal: 2.95 ± 0.436
0.69LysTrp: 0.69 ± 0.231
1.004LysTyr: 1.004 ± 0.254
0.0LysXaa: 0.0 ± 0.0
Leu
9.667LeuAla: 9.667 ± 0.821
0.251LeuCys: 0.251 ± 0.103
6.403LeuAsp: 6.403 ± 0.521
5.084LeuGlu: 5.084 ± 0.574
2.26LeuPhe: 2.26 ± 0.363
7.721LeuGly: 7.721 ± 0.654
1.506LeuHis: 1.506 ± 0.341
4.645LeuIle: 4.645 ± 0.51
3.515LeuLys: 3.515 ± 0.515
6.34LeuLeu: 6.34 ± 0.713
1.569LeuMet: 1.569 ± 0.31
3.139LeuAsn: 3.139 ± 0.519
5.587LeuPro: 5.587 ± 0.554
2.825LeuGln: 2.825 ± 0.509
6.152LeuArg: 6.152 ± 0.564
5.524LeuSer: 5.524 ± 0.523
6.214LeuThr: 6.214 ± 0.453
4.582LeuVal: 4.582 ± 0.569
1.193LeuTrp: 1.193 ± 0.333
2.511LeuTyr: 2.511 ± 0.448
0.0LeuXaa: 0.0 ± 0.0
Met
2.323MetAla: 2.323 ± 0.338
0.0MetCys: 0.0 ± 0.0
1.255MetAsp: 1.255 ± 0.317
1.506MetGlu: 1.506 ± 0.331
0.816MetPhe: 0.816 ± 0.2
1.758MetGly: 1.758 ± 0.359
0.377MetHis: 0.377 ± 0.162
0.753MetIle: 0.753 ± 0.216
1.004MetLys: 1.004 ± 0.279
1.193MetLeu: 1.193 ± 0.262
0.188MetMet: 0.188 ± 0.111
0.942MetAsn: 0.942 ± 0.194
0.879MetPro: 0.879 ± 0.252
0.753MetGln: 0.753 ± 0.188
1.067MetArg: 1.067 ± 0.248
2.197MetSer: 2.197 ± 0.385
1.946MetThr: 1.946 ± 0.271
1.13MetVal: 1.13 ± 0.331
0.188MetTrp: 0.188 ± 0.105
0.377MetTyr: 0.377 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
2.887AsnAla: 2.887 ± 0.592
0.0AsnCys: 0.0 ± 0.0
1.946AsnAsp: 1.946 ± 0.33
1.695AsnGlu: 1.695 ± 0.352
0.942AsnPhe: 0.942 ± 0.31
3.076AsnGly: 3.076 ± 0.43
0.816AsnHis: 0.816 ± 0.217
1.82AsnIle: 1.82 ± 0.325
0.628AsnLys: 0.628 ± 0.237
2.574AsnLeu: 2.574 ± 0.386
0.628AsnMet: 0.628 ± 0.166
0.816AsnAsn: 0.816 ± 0.218
2.636AsnPro: 2.636 ± 0.381
1.004AsnGln: 1.004 ± 0.235
1.255AsnArg: 1.255 ± 0.288
2.134AsnSer: 2.134 ± 0.42
1.946AsnThr: 1.946 ± 0.343
2.574AsnVal: 2.574 ± 0.394
0.816AsnTrp: 0.816 ± 0.196
1.255AsnTyr: 1.255 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
5.273ProAla: 5.273 ± 0.685
0.377ProCys: 0.377 ± 0.148
3.766ProAsp: 3.766 ± 0.432
4.143ProGlu: 4.143 ± 0.439
2.197ProPhe: 2.197 ± 0.374
4.833ProGly: 4.833 ± 0.661
1.067ProHis: 1.067 ± 0.301
2.448ProIle: 2.448 ± 0.459
2.071ProLys: 2.071 ± 0.259
4.457ProLeu: 4.457 ± 0.562
1.004ProMet: 1.004 ± 0.271
1.82ProAsn: 1.82 ± 0.357
3.013ProPro: 3.013 ± 0.487
1.695ProGln: 1.695 ± 0.352
3.013ProArg: 3.013 ± 0.478
3.829ProSer: 3.829 ± 0.529
4.08ProThr: 4.08 ± 0.54
3.766ProVal: 3.766 ± 0.429
0.753ProTrp: 0.753 ± 0.276
1.506ProTyr: 1.506 ± 0.329
0.0ProXaa: 0.0 ± 0.0
Gln
2.699GlnAla: 2.699 ± 0.415
0.063GlnCys: 0.063 ± 0.064
1.444GlnAsp: 1.444 ± 0.347
1.758GlnGlu: 1.758 ± 0.288
1.255GlnPhe: 1.255 ± 0.236
2.574GlnGly: 2.574 ± 0.385
0.502GlnHis: 0.502 ± 0.16
2.825GlnIle: 2.825 ± 0.521
1.13GlnLys: 1.13 ± 0.261
4.017GlnLeu: 4.017 ± 0.508
0.565GlnMet: 0.565 ± 0.184
0.565GlnAsn: 0.565 ± 0.152
1.695GlnPro: 1.695 ± 0.315
1.883GlnGln: 1.883 ± 0.379
1.758GlnArg: 1.758 ± 0.314
1.82GlnSer: 1.82 ± 0.357
2.071GlnThr: 2.071 ± 0.34
2.95GlnVal: 2.95 ± 0.367
0.69GlnTrp: 0.69 ± 0.15
0.439GlnTyr: 0.439 ± 0.146
0.0GlnXaa: 0.0 ± 0.0
Arg
4.708ArgAla: 4.708 ± 0.688
0.628ArgCys: 0.628 ± 0.25
3.641ArgAsp: 3.641 ± 0.422
4.896ArgGlu: 4.896 ± 0.567
2.009ArgPhe: 2.009 ± 0.349
4.771ArgGly: 4.771 ± 0.591
1.255ArgHis: 1.255 ± 0.294
3.139ArgIle: 3.139 ± 0.452
3.076ArgLys: 3.076 ± 0.539
6.152ArgLeu: 6.152 ± 0.643
1.946ArgMet: 1.946 ± 0.372
2.26ArgAsn: 2.26 ± 0.384
2.699ArgPro: 2.699 ± 0.522
1.569ArgGln: 1.569 ± 0.326
5.398ArgArg: 5.398 ± 0.735
3.515ArgSer: 3.515 ± 0.481
2.95ArgThr: 2.95 ± 0.477
5.084ArgVal: 5.084 ± 0.588
1.255ArgTrp: 1.255 ± 0.317
1.883ArgTyr: 1.883 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
6.654SerAla: 6.654 ± 0.929
0.565SerCys: 0.565 ± 0.212
3.766SerAsp: 3.766 ± 0.476
4.017SerGlu: 4.017 ± 0.452
1.946SerPhe: 1.946 ± 0.374
6.152SerGly: 6.152 ± 0.68
1.381SerHis: 1.381 ± 0.289
2.636SerIle: 2.636 ± 0.494
2.071SerLys: 2.071 ± 0.395
5.649SerLeu: 5.649 ± 0.549
1.506SerMet: 1.506 ± 0.297
2.071SerAsn: 2.071 ± 0.472
2.699SerPro: 2.699 ± 0.423
2.385SerGln: 2.385 ± 0.335
3.013SerArg: 3.013 ± 0.393
4.143SerSer: 4.143 ± 0.731
3.264SerThr: 3.264 ± 0.428
4.08SerVal: 4.08 ± 0.47
1.381SerTrp: 1.381 ± 0.308
1.444SerTyr: 1.444 ± 0.337
0.0SerXaa: 0.0 ± 0.0
Thr
5.273ThrAla: 5.273 ± 0.721
0.126ThrCys: 0.126 ± 0.086
4.08ThrAsp: 4.08 ± 0.588
4.017ThrGlu: 4.017 ± 0.614
2.071ThrPhe: 2.071 ± 0.389
6.905ThrGly: 6.905 ± 0.526
0.942ThrHis: 0.942 ± 0.264
2.699ThrIle: 2.699 ± 0.511
2.762ThrLys: 2.762 ± 0.331
5.21ThrLeu: 5.21 ± 0.632
0.879ThrMet: 0.879 ± 0.195
1.695ThrAsn: 1.695 ± 0.332
3.892ThrPro: 3.892 ± 0.533
1.82ThrGln: 1.82 ± 0.327
3.578ThrArg: 3.578 ± 0.594
3.578ThrSer: 3.578 ± 0.602
4.331ThrThr: 4.331 ± 0.618
5.775ThrVal: 5.775 ± 0.691
1.318ThrTrp: 1.318 ± 0.297
2.134ThrTyr: 2.134 ± 0.358
0.0ThrXaa: 0.0 ± 0.0
Val
7.093ValAla: 7.093 ± 0.728
0.377ValCys: 0.377 ± 0.173
5.084ValAsp: 5.084 ± 0.627
4.833ValGlu: 4.833 ± 0.581
2.448ValPhe: 2.448 ± 0.324
4.708ValGly: 4.708 ± 0.586
1.444ValHis: 1.444 ± 0.238
4.017ValIle: 4.017 ± 0.658
2.95ValLys: 2.95 ± 0.428
5.649ValLeu: 5.649 ± 0.624
1.067ValMet: 1.067 ± 0.3
2.699ValAsn: 2.699 ± 0.412
4.268ValPro: 4.268 ± 0.515
2.197ValGln: 2.197 ± 0.405
5.147ValArg: 5.147 ± 0.654
4.645ValSer: 4.645 ± 0.418
5.649ValThr: 5.649 ± 0.516
5.21ValVal: 5.21 ± 0.653
1.255ValTrp: 1.255 ± 0.273
2.197ValTyr: 2.197 ± 0.339
0.0ValXaa: 0.0 ± 0.0
Trp
1.695TrpAla: 1.695 ± 0.34
0.188TrpCys: 0.188 ± 0.088
1.695TrpAsp: 1.695 ± 0.29
0.879TrpGlu: 0.879 ± 0.191
0.879TrpPhe: 0.879 ± 0.256
1.569TrpGly: 1.569 ± 0.284
0.377TrpHis: 0.377 ± 0.151
1.004TrpIle: 1.004 ± 0.203
0.377TrpLys: 0.377 ± 0.166
1.883TrpLeu: 1.883 ± 0.312
0.439TrpMet: 0.439 ± 0.18
0.502TrpAsn: 0.502 ± 0.166
1.004TrpPro: 1.004 ± 0.236
1.004TrpGln: 1.004 ± 0.197
1.255TrpArg: 1.255 ± 0.311
1.004TrpSer: 1.004 ± 0.201
1.569TrpThr: 1.569 ± 0.374
1.883TrpVal: 1.883 ± 0.326
0.69TrpTrp: 0.69 ± 0.261
0.377TrpTyr: 0.377 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.762TyrAla: 2.762 ± 0.404
0.251TyrCys: 0.251 ± 0.156
1.255TyrAsp: 1.255 ± 0.26
2.134TyrGlu: 2.134 ± 0.335
0.69TyrPhe: 0.69 ± 0.178
2.323TyrGly: 2.323 ± 0.404
0.753TyrHis: 0.753 ± 0.224
1.632TyrIle: 1.632 ± 0.364
1.193TyrLys: 1.193 ± 0.279
2.511TyrLeu: 2.511 ± 0.466
0.69TyrMet: 0.69 ± 0.184
1.381TyrAsn: 1.381 ± 0.316
1.444TyrPro: 1.444 ± 0.276
1.13TyrGln: 1.13 ± 0.303
2.197TyrArg: 2.197 ± 0.327
1.444TyrSer: 1.444 ± 0.274
2.071TyrThr: 2.071 ± 0.334
1.883TyrVal: 1.883 ± 0.382
0.377TyrTrp: 0.377 ± 0.168
0.628TyrTyr: 0.628 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (15932 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski