Rhizobium phage 16-3 (Bacteriophage 16-3)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4UTQ7|B4UTQ7_BP163 p018 OS=Rhizobium phage 16-3 OX=10704 GN=16-3p018 PE=4 SV=1
MM1 pKa = 7.61GFTPRR6 pKa = 11.84AIDD9 pKa = 6.13DD10 pKa = 3.56MTLWEE15 pKa = 5.02FDD17 pKa = 3.51CCYY20 pKa = 10.2EE21 pKa = 4.64GYY23 pKa = 9.99RR24 pKa = 11.84KK25 pKa = 9.34SHH27 pKa = 4.94QTEE30 pKa = 3.8EE31 pKa = 4.27DD32 pKa = 3.75APPPMADD39 pKa = 3.2EE40 pKa = 4.18QLAEE44 pKa = 4.52LGIQGFF50 pKa = 3.94

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4UTW0|B4UTW0_BP163 p072 OS=Rhizobium phage 16-3 OX=10704 GN=16-3p072 PE=4 SV=1
MM1 pKa = 7.43NSLHH5 pKa = 6.85IVDD8 pKa = 5.18RR9 pKa = 11.84DD10 pKa = 3.61PVAQRR15 pKa = 11.84VAAYY19 pKa = 8.98FRR21 pKa = 11.84SSVFEE26 pKa = 3.92RR27 pKa = 11.84FEE29 pKa = 3.85ATKK32 pKa = 10.61PRR34 pKa = 11.84DD35 pKa = 3.31STLAVHH41 pKa = 6.28HH42 pKa = 6.58HH43 pKa = 6.79LVPDD47 pKa = 4.06LRR49 pKa = 11.84PDD51 pKa = 2.93GWIQPGFRR59 pKa = 11.84ADD61 pKa = 3.71EE62 pKa = 4.31EE63 pKa = 4.93GGLHH67 pKa = 6.75DD68 pKa = 5.44LGVNRR73 pKa = 11.84YY74 pKa = 8.49CVAAGGNARR83 pKa = 11.84TATASVAGAGRR94 pKa = 11.84VEE96 pKa = 4.17LEE98 pKa = 3.78FAFFSVGNAARR109 pKa = 11.84FRR111 pKa = 11.84YY112 pKa = 8.63WRR114 pKa = 11.84DD115 pKa = 2.75ARR117 pKa = 11.84QLRR120 pKa = 11.84VACTGNGFLAKK131 pKa = 9.41LTRR134 pKa = 11.84HH135 pKa = 6.03IDD137 pKa = 3.46RR138 pKa = 11.84YY139 pKa = 8.6VQPSGDD145 pKa = 3.84CGVPIRR151 pKa = 11.84PRR153 pKa = 11.84YY154 pKa = 9.49RR155 pKa = 11.84AMLPRR160 pKa = 11.84AIGLLKK166 pKa = 10.89DD167 pKa = 3.4SDD169 pKa = 4.12FLRR172 pKa = 11.84SLVVGQASLGLFIQQHH188 pKa = 4.81LRR190 pKa = 11.84KK191 pKa = 9.78AA192 pKa = 3.54

Molecular weight: 21.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

1

110

19559

40

859

177.8

19.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.672 ± 0.338

1.248 ± 0.127

6.105 ± 0.195

6.437 ± 0.216

3.446 ± 0.146

8.283 ± 0.272

2.193 ± 0.161

4.709 ± 0.154

4.959 ± 0.26

7.633 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.133

3.19 ± 0.176

4.642 ± 0.244

3.354 ± 0.204

7.05 ± 0.311

5.941 ± 0.23

5.333 ± 0.228

6.969 ± 0.234

1.81 ± 0.117

2.674 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski