Clostridium phage phiCD111

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Leicestervirus; Clostridioides virus phiCD111

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8WIK0|A0A0A8WIK0_9CAUD Uncharacterized protein OS=Clostridium phage phiCD111 OX=1582150 GN=PHICD111_20048 PE=4 SV=1
MM1 pKa = 7.64SEE3 pKa = 5.36FVQCCCCEE11 pKa = 3.56RR12 pKa = 11.84TINIEE17 pKa = 3.74EE18 pKa = 4.15NNYY21 pKa = 8.78VQYY24 pKa = 10.76EE25 pKa = 4.18KK26 pKa = 10.84EE27 pKa = 3.89ALGLVFTLYY36 pKa = 10.73FCLNCVDD43 pKa = 4.63EE44 pKa = 4.67LSEE47 pKa = 4.15MEE49 pKa = 4.11

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8WIJ6|A0A0A8WIJ6_9CAUD Uncharacterized protein OS=Clostridium phage phiCD111 OX=1582150 GN=PHICD111_20038 PE=4 SV=1
MM1 pKa = 8.26DD2 pKa = 4.88NFLLNILAGVIASLIFYY19 pKa = 9.98IISKK23 pKa = 9.74LFRR26 pKa = 11.84QVKK29 pKa = 8.87NHH31 pKa = 5.97SAQKK35 pKa = 10.51SGWEE39 pKa = 3.7FDD41 pKa = 4.05LKK43 pKa = 10.85IKK45 pKa = 10.36FKK47 pKa = 10.76KK48 pKa = 10.25SRR50 pKa = 3.35

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12303

39

1767

223.7

25.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.608 ± 0.394

0.983 ± 0.163

5.934 ± 0.249

8.884 ± 0.403

4.349 ± 0.29

5.934 ± 0.463

0.918 ± 0.154

9.063 ± 0.315

10.087 ± 0.404

7.957 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.174

7.307 ± 0.339

1.796 ± 0.15

2.991 ± 0.22

3.316 ± 0.294

6.429 ± 0.289

5.722 ± 0.388

5.527 ± 0.219

0.983 ± 0.121

4.056 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski