Amino acid dipepetide frequency for Mycobacterium phage Alvin

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.448AlaAla: 13.448 ± 1.606
0.772AlaCys: 0.772 ± 0.186
6.949AlaAsp: 6.949 ± 0.873
6.113AlaGlu: 6.113 ± 0.692
3.089AlaPhe: 3.089 ± 0.538
7.4AlaGly: 7.4 ± 0.792
1.673AlaHis: 1.673 ± 0.436
4.118AlaIle: 4.118 ± 0.59
3.861AlaLys: 3.861 ± 0.531
9.073AlaLeu: 9.073 ± 0.892
2.316AlaMet: 2.316 ± 0.404
2.638AlaAsn: 2.638 ± 0.318
4.762AlaPro: 4.762 ± 0.72
3.089AlaGln: 3.089 ± 0.456
5.855AlaArg: 5.855 ± 0.504
5.341AlaSer: 5.341 ± 0.658
5.791AlaThr: 5.791 ± 0.59
8.429AlaVal: 8.429 ± 0.751
1.995AlaTrp: 1.995 ± 0.395
3.089AlaTyr: 3.089 ± 0.481
0.0AlaXaa: 0.0 ± 0.0
Cys
0.836CysAla: 0.836 ± 0.301
0.064CysCys: 0.064 ± 0.067
0.515CysAsp: 0.515 ± 0.178
0.836CysGlu: 0.836 ± 0.2
0.193CysPhe: 0.193 ± 0.115
0.579CysGly: 0.579 ± 0.228
0.129CysHis: 0.129 ± 0.086
0.257CysIle: 0.257 ± 0.126
0.129CysLys: 0.129 ± 0.095
0.322CysLeu: 0.322 ± 0.177
0.064CysMet: 0.064 ± 0.059
0.322CysAsn: 0.322 ± 0.141
0.193CysPro: 0.193 ± 0.113
0.129CysGln: 0.129 ± 0.098
0.515CysArg: 0.515 ± 0.172
0.322CysSer: 0.322 ± 0.138
0.257CysThr: 0.257 ± 0.127
0.129CysVal: 0.129 ± 0.083
0.257CysTrp: 0.257 ± 0.123
0.322CysTyr: 0.322 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
6.563AspAla: 6.563 ± 0.669
0.45AspCys: 0.45 ± 0.16
4.955AspAsp: 4.955 ± 0.493
3.861AspGlu: 3.861 ± 0.458
2.059AspPhe: 2.059 ± 0.342
6.242AspGly: 6.242 ± 0.579
1.094AspHis: 1.094 ± 0.29
2.638AspIle: 2.638 ± 0.476
2.638AspLys: 2.638 ± 0.417
6.692AspLeu: 6.692 ± 0.687
1.223AspMet: 1.223 ± 0.221
1.93AspAsn: 1.93 ± 0.316
4.633AspPro: 4.633 ± 0.61
1.544AspGln: 1.544 ± 0.326
3.989AspArg: 3.989 ± 0.467
3.475AspSer: 3.475 ± 0.469
3.796AspThr: 3.796 ± 0.435
5.019AspVal: 5.019 ± 0.589
1.609AspTrp: 1.609 ± 0.332
1.802AspTyr: 1.802 ± 0.355
0.0AspXaa: 0.0 ± 0.0
Glu
5.791GluAla: 5.791 ± 0.743
0.45GluCys: 0.45 ± 0.17
5.083GluAsp: 5.083 ± 0.603
5.083GluGlu: 5.083 ± 0.573
2.123GluPhe: 2.123 ± 0.386
3.989GluGly: 3.989 ± 0.427
1.094GluHis: 1.094 ± 0.304
3.217GluIle: 3.217 ± 0.465
2.896GluLys: 2.896 ± 0.504
7.078GluLeu: 7.078 ± 0.667
1.609GluMet: 1.609 ± 0.318
1.737GluAsn: 1.737 ± 0.405
2.831GluPro: 2.831 ± 0.456
2.316GluGln: 2.316 ± 0.408
3.41GluArg: 3.41 ± 0.574
3.475GluSer: 3.475 ± 0.419
3.861GluThr: 3.861 ± 0.473
5.598GluVal: 5.598 ± 0.555
1.737GluTrp: 1.737 ± 0.438
2.574GluTyr: 2.574 ± 0.436
0.0GluXaa: 0.0 ± 0.0
Phe
2.316PheAla: 2.316 ± 0.31
0.257PheCys: 0.257 ± 0.165
2.574PheAsp: 2.574 ± 0.43
2.188PheGlu: 2.188 ± 0.354
0.515PhePhe: 0.515 ± 0.151
3.539PheGly: 3.539 ± 0.538
0.579PheHis: 0.579 ± 0.25
1.544PheIle: 1.544 ± 0.302
1.351PheLys: 1.351 ± 0.283
2.316PheLeu: 2.316 ± 0.426
0.579PheMet: 0.579 ± 0.2
1.094PheAsn: 1.094 ± 0.26
1.609PhePro: 1.609 ± 0.314
0.772PheGln: 0.772 ± 0.175
1.866PheArg: 1.866 ± 0.384
2.059PheSer: 2.059 ± 0.519
1.673PheThr: 1.673 ± 0.346
2.188PheVal: 2.188 ± 0.377
0.643PheTrp: 0.643 ± 0.232
0.836PheTyr: 0.836 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
6.949GlyAla: 6.949 ± 0.882
0.515GlyCys: 0.515 ± 0.153
5.855GlyAsp: 5.855 ± 0.534
4.955GlyGlu: 4.955 ± 0.544
3.282GlyPhe: 3.282 ± 0.489
9.459GlyGly: 9.459 ± 1.938
1.93GlyHis: 1.93 ± 0.414
4.376GlyIle: 4.376 ± 0.707
3.732GlyLys: 3.732 ± 0.531
7.915GlyLeu: 7.915 ± 0.991
1.416GlyMet: 1.416 ± 0.292
2.896GlyAsn: 2.896 ± 0.419
3.732GlyPro: 3.732 ± 0.488
2.574GlyGln: 2.574 ± 0.433
5.148GlyArg: 5.148 ± 0.533
6.563GlySer: 6.563 ± 0.942
5.855GlyThr: 5.855 ± 0.733
5.791GlyVal: 5.791 ± 0.773
2.316GlyTrp: 2.316 ± 0.378
2.96GlyTyr: 2.96 ± 0.439
0.0GlyXaa: 0.0 ± 0.0
His
1.673HisAla: 1.673 ± 0.336
0.129HisCys: 0.129 ± 0.105
1.351HisAsp: 1.351 ± 0.265
1.48HisGlu: 1.48 ± 0.31
0.579HisPhe: 0.579 ± 0.193
1.544HisGly: 1.544 ± 0.354
0.708HisHis: 0.708 ± 0.194
0.965HisIle: 0.965 ± 0.228
1.223HisLys: 1.223 ± 0.315
1.544HisLeu: 1.544 ± 0.365
0.129HisMet: 0.129 ± 0.089
0.386HisAsn: 0.386 ± 0.176
1.287HisPro: 1.287 ± 0.248
0.901HisGln: 0.901 ± 0.201
1.287HisArg: 1.287 ± 0.261
0.579HisSer: 0.579 ± 0.184
0.965HisThr: 0.965 ± 0.311
1.609HisVal: 1.609 ± 0.306
0.515HisTrp: 0.515 ± 0.177
0.772HisTyr: 0.772 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
6.306IleAla: 6.306 ± 0.775
0.322IleCys: 0.322 ± 0.141
3.217IleAsp: 3.217 ± 0.355
3.282IleGlu: 3.282 ± 0.408
0.901IlePhe: 0.901 ± 0.215
3.925IleGly: 3.925 ± 0.504
0.901IleHis: 0.901 ± 0.249
1.737IleIle: 1.737 ± 0.333
1.673IleLys: 1.673 ± 0.41
3.475IleLeu: 3.475 ± 0.469
0.643IleMet: 0.643 ± 0.161
1.802IleAsn: 1.802 ± 0.383
3.282IlePro: 3.282 ± 0.448
1.544IleGln: 1.544 ± 0.391
3.603IleArg: 3.603 ± 0.496
3.217IleSer: 3.217 ± 0.459
3.153IleThr: 3.153 ± 0.51
3.346IleVal: 3.346 ± 0.532
0.836IleTrp: 0.836 ± 0.186
1.609IleTyr: 1.609 ± 0.279
0.0IleXaa: 0.0 ± 0.0
Lys
3.603LysAla: 3.603 ± 0.462
0.193LysCys: 0.193 ± 0.108
2.188LysAsp: 2.188 ± 0.402
2.381LysGlu: 2.381 ± 0.444
1.673LysPhe: 1.673 ± 0.278
2.831LysGly: 2.831 ± 0.396
1.158LysHis: 1.158 ± 0.324
2.574LysIle: 2.574 ± 0.509
1.93LysLys: 1.93 ± 0.405
3.475LysLeu: 3.475 ± 0.448
1.03LysMet: 1.03 ± 0.213
1.544LysAsn: 1.544 ± 0.326
2.896LysPro: 2.896 ± 0.419
1.416LysGln: 1.416 ± 0.298
3.024LysArg: 3.024 ± 0.488
2.188LysSer: 2.188 ± 0.375
2.638LysThr: 2.638 ± 0.372
2.703LysVal: 2.703 ± 0.447
0.708LysTrp: 0.708 ± 0.222
1.03LysTyr: 1.03 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
9.588LeuAla: 9.588 ± 0.968
0.45LeuCys: 0.45 ± 0.186
6.177LeuAsp: 6.177 ± 0.622
5.598LeuGlu: 5.598 ± 0.7
1.866LeuPhe: 1.866 ± 0.423
8.172LeuGly: 8.172 ± 0.884
1.609LeuHis: 1.609 ± 0.322
4.89LeuIle: 4.89 ± 0.605
3.925LeuLys: 3.925 ± 0.502
6.177LeuLeu: 6.177 ± 0.587
1.995LeuMet: 1.995 ± 0.357
3.024LeuAsn: 3.024 ± 0.446
5.855LeuPro: 5.855 ± 0.628
2.445LeuGln: 2.445 ± 0.409
6.435LeuArg: 6.435 ± 0.593
5.469LeuSer: 5.469 ± 0.615
5.92LeuThr: 5.92 ± 0.533
4.826LeuVal: 4.826 ± 0.741
0.836LeuTrp: 0.836 ± 0.258
2.381LeuTyr: 2.381 ± 0.487
0.0LeuXaa: 0.0 ± 0.0
Met
2.381MetAla: 2.381 ± 0.352
0.0MetCys: 0.0 ± 0.0
1.158MetAsp: 1.158 ± 0.286
1.737MetGlu: 1.737 ± 0.324
0.45MetPhe: 0.45 ± 0.171
1.287MetGly: 1.287 ± 0.29
0.386MetHis: 0.386 ± 0.156
0.579MetIle: 0.579 ± 0.191
1.03MetLys: 1.03 ± 0.247
1.48MetLeu: 1.48 ± 0.339
0.064MetMet: 0.064 ± 0.059
0.901MetAsn: 0.901 ± 0.2
1.158MetPro: 1.158 ± 0.268
0.708MetGln: 0.708 ± 0.214
1.223MetArg: 1.223 ± 0.277
2.059MetSer: 2.059 ± 0.446
2.316MetThr: 2.316 ± 0.377
0.901MetVal: 0.901 ± 0.214
0.257MetTrp: 0.257 ± 0.11
0.45MetTyr: 0.45 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
2.767AsnAla: 2.767 ± 0.462
0.129AsnCys: 0.129 ± 0.094
1.737AsnAsp: 1.737 ± 0.413
1.609AsnGlu: 1.609 ± 0.291
0.836AsnPhe: 0.836 ± 0.235
3.668AsnGly: 3.668 ± 0.501
0.579AsnHis: 0.579 ± 0.168
1.351AsnIle: 1.351 ± 0.302
0.45AsnLys: 0.45 ± 0.15
2.445AsnLeu: 2.445 ± 0.313
0.772AsnMet: 0.772 ± 0.217
0.901AsnAsn: 0.901 ± 0.207
2.896AsnPro: 2.896 ± 0.416
1.287AsnGln: 1.287 ± 0.376
1.416AsnArg: 1.416 ± 0.285
1.802AsnSer: 1.802 ± 0.407
2.059AsnThr: 2.059 ± 0.35
2.896AsnVal: 2.896 ± 0.485
0.708AsnTrp: 0.708 ± 0.197
1.544AsnTyr: 1.544 ± 0.284
0.0AsnXaa: 0.0 ± 0.0
Pro
5.341ProAla: 5.341 ± 0.578
0.386ProCys: 0.386 ± 0.148
4.44ProAsp: 4.44 ± 0.531
4.762ProGlu: 4.762 ± 0.56
2.123ProPhe: 2.123 ± 0.386
5.083ProGly: 5.083 ± 0.606
0.901ProHis: 0.901 ± 0.265
2.252ProIle: 2.252 ± 0.388
2.188ProLys: 2.188 ± 0.303
4.569ProLeu: 4.569 ± 0.6
0.965ProMet: 0.965 ± 0.264
1.416ProAsn: 1.416 ± 0.305
3.089ProPro: 3.089 ± 0.48
1.48ProGln: 1.48 ± 0.305
2.831ProArg: 2.831 ± 0.45
3.539ProSer: 3.539 ± 0.509
4.118ProThr: 4.118 ± 0.502
3.668ProVal: 3.668 ± 0.497
0.772ProTrp: 0.772 ± 0.297
1.351ProTyr: 1.351 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
2.767GlnAla: 2.767 ± 0.507
0.129GlnCys: 0.129 ± 0.089
1.416GlnAsp: 1.416 ± 0.348
1.223GlnGlu: 1.223 ± 0.282
1.094GlnPhe: 1.094 ± 0.236
2.638GlnGly: 2.638 ± 0.3
0.579GlnHis: 0.579 ± 0.198
2.703GlnIle: 2.703 ± 0.477
0.836GlnLys: 0.836 ± 0.212
3.603GlnLeu: 3.603 ± 0.479
0.901GlnMet: 0.901 ± 0.235
0.579GlnAsn: 0.579 ± 0.245
1.802GlnPro: 1.802 ± 0.32
1.737GlnGln: 1.737 ± 0.439
1.802GlnArg: 1.802 ± 0.393
1.802GlnSer: 1.802 ± 0.309
1.609GlnThr: 1.609 ± 0.304
2.574GlnVal: 2.574 ± 0.339
0.643GlnTrp: 0.643 ± 0.197
0.515GlnTyr: 0.515 ± 0.183
0.0GlnXaa: 0.0 ± 0.0
Arg
5.534ArgAla: 5.534 ± 0.576
0.772ArgCys: 0.772 ± 0.259
3.089ArgAsp: 3.089 ± 0.47
4.762ArgGlu: 4.762 ± 0.766
1.866ArgPhe: 1.866 ± 0.401
4.89ArgGly: 4.89 ± 0.658
1.158ArgHis: 1.158 ± 0.228
3.346ArgIle: 3.346 ± 0.562
3.089ArgLys: 3.089 ± 0.509
6.242ArgLeu: 6.242 ± 0.856
1.995ArgMet: 1.995 ± 0.35
2.059ArgAsn: 2.059 ± 0.508
1.995ArgPro: 1.995 ± 0.343
1.802ArgGln: 1.802 ± 0.343
5.148ArgArg: 5.148 ± 0.657
3.796ArgSer: 3.796 ± 0.492
3.089ArgThr: 3.089 ± 0.499
5.341ArgVal: 5.341 ± 0.629
1.158ArgTrp: 1.158 ± 0.273
1.802ArgTyr: 1.802 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
6.435SerAla: 6.435 ± 0.765
0.45SerCys: 0.45 ± 0.216
3.732SerAsp: 3.732 ± 0.424
3.668SerGlu: 3.668 ± 0.593
1.737SerPhe: 1.737 ± 0.346
6.628SerGly: 6.628 ± 1.026
1.737SerHis: 1.737 ± 0.316
3.153SerIle: 3.153 ± 0.526
2.574SerLys: 2.574 ± 0.486
4.89SerLeu: 4.89 ± 0.525
1.416SerMet: 1.416 ± 0.301
2.316SerAsn: 2.316 ± 0.465
3.41SerPro: 3.41 ± 0.582
1.544SerGln: 1.544 ± 0.322
2.767SerArg: 2.767 ± 0.321
3.41SerSer: 3.41 ± 0.722
3.41SerThr: 3.41 ± 0.559
3.668SerVal: 3.668 ± 0.419
1.287SerTrp: 1.287 ± 0.305
1.094SerTyr: 1.094 ± 0.26
0.0SerXaa: 0.0 ± 0.0
Thr
6.435ThrAla: 6.435 ± 0.639
0.129ThrCys: 0.129 ± 0.084
3.732ThrAsp: 3.732 ± 0.493
4.504ThrGlu: 4.504 ± 0.528
1.93ThrPhe: 1.93 ± 0.31
6.628ThrGly: 6.628 ± 0.619
1.03ThrHis: 1.03 ± 0.341
2.831ThrIle: 2.831 ± 0.586
2.831ThrLys: 2.831 ± 0.412
6.113ThrLeu: 6.113 ± 0.627
1.223ThrMet: 1.223 ± 0.236
1.737ThrAsn: 1.737 ± 0.402
3.668ThrPro: 3.668 ± 0.53
1.93ThrGln: 1.93 ± 0.314
3.346ThrArg: 3.346 ± 0.598
3.41ThrSer: 3.41 ± 0.493
4.182ThrThr: 4.182 ± 0.61
5.019ThrVal: 5.019 ± 0.624
1.287ThrTrp: 1.287 ± 0.265
1.93ThrTyr: 1.93 ± 0.319
0.0ThrXaa: 0.0 ± 0.0
Val
7.207ValAla: 7.207 ± 0.81
0.322ValCys: 0.322 ± 0.134
5.212ValAsp: 5.212 ± 0.555
4.569ValGlu: 4.569 ± 0.552
2.767ValPhe: 2.767 ± 0.47
5.405ValGly: 5.405 ± 0.82
1.351ValHis: 1.351 ± 0.251
3.346ValIle: 3.346 ± 0.504
3.153ValLys: 3.153 ± 0.423
5.598ValLeu: 5.598 ± 0.622
1.223ValMet: 1.223 ± 0.286
2.703ValAsn: 2.703 ± 0.39
3.925ValPro: 3.925 ± 0.501
1.93ValGln: 1.93 ± 0.369
5.405ValArg: 5.405 ± 0.729
4.762ValSer: 4.762 ± 0.559
5.469ValThr: 5.469 ± 0.56
4.697ValVal: 4.697 ± 0.699
1.223ValTrp: 1.223 ± 0.257
1.995ValTyr: 1.995 ± 0.358
0.0ValXaa: 0.0 ± 0.0
Trp
1.48TrpAla: 1.48 ± 0.333
0.257TrpCys: 0.257 ± 0.116
1.544TrpAsp: 1.544 ± 0.269
1.03TrpGlu: 1.03 ± 0.228
0.836TrpPhe: 0.836 ± 0.257
1.48TrpGly: 1.48 ± 0.314
0.515TrpHis: 0.515 ± 0.193
1.287TrpIle: 1.287 ± 0.286
0.45TrpLys: 0.45 ± 0.255
1.93TrpLeu: 1.93 ± 0.294
0.257TrpMet: 0.257 ± 0.14
0.579TrpAsn: 0.579 ± 0.196
0.772TrpPro: 0.772 ± 0.237
0.708TrpGln: 0.708 ± 0.194
1.158TrpArg: 1.158 ± 0.365
0.836TrpSer: 0.836 ± 0.225
1.802TrpThr: 1.802 ± 0.367
2.059TrpVal: 2.059 ± 0.298
0.579TrpTrp: 0.579 ± 0.184
0.257TrpTyr: 0.257 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.381TyrAla: 2.381 ± 0.387
0.193TyrCys: 0.193 ± 0.109
1.287TyrAsp: 1.287 ± 0.281
2.316TyrGlu: 2.316 ± 0.371
0.579TyrPhe: 0.579 ± 0.179
2.831TyrGly: 2.831 ± 0.434
0.515TyrHis: 0.515 ± 0.189
1.609TyrIle: 1.609 ± 0.34
1.223TyrLys: 1.223 ± 0.268
2.703TyrLeu: 2.703 ± 0.422
0.643TyrMet: 0.643 ± 0.179
1.287TyrAsn: 1.287 ± 0.295
1.48TyrPro: 1.48 ± 0.286
1.094TyrGln: 1.094 ± 0.278
2.703TyrArg: 2.703 ± 0.392
1.287TyrSer: 1.287 ± 0.269
1.93TyrThr: 1.93 ± 0.403
1.802TyrVal: 1.802 ± 0.374
0.45TyrTrp: 0.45 ± 0.168
0.579TyrTyr: 0.579 ± 0.198
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (15542 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski