Agromyces sp. MF30-A

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S0WX10|A0A2S0WX10_9MICO DUF4350 domain-containing protein OS=Agromyces sp. MF30-A OX=2080742 GN=DCE93_09575 PE=4 SV=1
MM1 pKa = 7.35NSATRR6 pKa = 11.84RR7 pKa = 11.84ILTRR11 pKa = 11.84ALVSLAAVAVAIPLSGCSIVRR32 pKa = 11.84DD33 pKa = 3.87LVGGSAPQPEE43 pKa = 4.41RR44 pKa = 11.84DD45 pKa = 3.42EE46 pKa = 4.33TTQEE50 pKa = 3.73IVEE53 pKa = 4.56EE54 pKa = 4.34GDD56 pKa = 3.25ADD58 pKa = 3.96VFALIVGDD66 pKa = 3.86CMYY69 pKa = 10.71EE70 pKa = 3.98VTEE73 pKa = 4.17EE74 pKa = 4.16LVSEE78 pKa = 4.67VPVVPCDD85 pKa = 3.5QPHH88 pKa = 6.93DD89 pKa = 4.03DD90 pKa = 4.45EE91 pKa = 6.1VYY93 pKa = 10.83FDD95 pKa = 3.75LTIDD99 pKa = 3.65GDD101 pKa = 4.28EE102 pKa = 4.28YY103 pKa = 10.82PGDD106 pKa = 4.88DD107 pKa = 4.59VIQTQAEE114 pKa = 4.34EE115 pKa = 3.9ACLAEE120 pKa = 4.27FEE122 pKa = 4.76PFVGLAYY129 pKa = 10.42DD130 pKa = 3.9SSTLDD135 pKa = 3.3FSTYY139 pKa = 10.09RR140 pKa = 11.84PSHH143 pKa = 5.96EE144 pKa = 4.12SWEE147 pKa = 4.32TRR149 pKa = 11.84DD150 pKa = 4.01DD151 pKa = 3.82RR152 pKa = 11.84VVSCVIYY159 pKa = 10.69DD160 pKa = 3.55PAGQVTGTLAGAARR174 pKa = 4.02

Molecular weight: 18.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S0WTJ7|A0A2S0WTJ7_9MICO Uncharacterized protein OS=Agromyces sp. MF30-A OX=2080742 GN=DCE93_02440 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2774

0

2774

909229

29

2565

327.8

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.957 ± 0.071

0.468 ± 0.009

6.065 ± 0.037

6.057 ± 0.043

3.186 ± 0.026

9.116 ± 0.046

1.969 ± 0.021

4.546 ± 0.032

1.896 ± 0.034

10.099 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.688 ± 0.018

1.896 ± 0.026

5.402 ± 0.034

2.582 ± 0.024

7.401 ± 0.057

5.594 ± 0.032

5.692 ± 0.035

9.018 ± 0.041

1.455 ± 0.018

1.913 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski