Aspergillus welwitschiae
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13680 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3F3PWB2|A0A3F3PWB2_9EURO FAD binding domain-domain-containing protein OS=Aspergillus welwitschiae OX=1341132 GN=BDQ94DRAFT_160760 PE=3 SV=1
MM1 pKa = 7.07 KK2 pKa = 9.9 TYY4 pKa = 10.84 GLWSTLAALGSFTLSSAKK22 pKa = 9.68 TINFVAHH29 pKa = 7.08 EE30 pKa = 4.68 DD31 pKa = 3.7 DD32 pKa = 5.65 DD33 pKa = 5.68 LLFLSPDD40 pKa = 4.47 LIRR43 pKa = 11.84 DD44 pKa = 3.6 ILSGEE49 pKa = 4.39 PIRR52 pKa = 11.84 TVFLTAGDD60 pKa = 4.0 AGLGSDD66 pKa = 3.5 YY67 pKa = 11.0 WNARR71 pKa = 11.84 EE72 pKa = 4.0 EE73 pKa = 4.44 GSRR76 pKa = 11.84 AAYY79 pKa = 10.58 ARR81 pKa = 11.84 MAVASNNWDD90 pKa = 3.2 EE91 pKa = 5.09 DD92 pKa = 4.52 VIFVGDD98 pKa = 4.0 KK99 pKa = 10.94 SITLYY104 pKa = 10.31 TLQDD108 pKa = 4.01 DD109 pKa = 4.91 DD110 pKa = 5.76 DD111 pKa = 5.1 ISLAFMHH118 pKa = 6.84 LPDD121 pKa = 4.93 GNMDD125 pKa = 3.49 GTGFGPDD132 pKa = 3.85 QNDD135 pKa = 3.5 SLQKK139 pKa = 10.46 LWNGDD144 pKa = 2.49 IDD146 pKa = 4.34 VIRR149 pKa = 11.84 TIDD152 pKa = 3.45 GSGIEE157 pKa = 4.11 YY158 pKa = 9.48 TRR160 pKa = 11.84 DD161 pKa = 3.19 EE162 pKa = 4.5 LLEE165 pKa = 4.35 TIAALIDD172 pKa = 4.57 DD173 pKa = 5.25 FDD175 pKa = 5.85 PDD177 pKa = 3.61 EE178 pKa = 4.89 VKK180 pKa = 10.28 TGDD183 pKa = 3.6 YY184 pKa = 11.43 VNDD187 pKa = 4.35 FGDD190 pKa = 4.38 GDD192 pKa = 3.99 HH193 pKa = 7.18 SDD195 pKa = 4.75 HH196 pKa = 6.31 YY197 pKa = 10.96 ACGYY201 pKa = 10.12 FVDD204 pKa = 4.93 NALQQADD211 pKa = 3.89 SDD213 pKa = 4.19 AGLTGYY219 pKa = 10.12 YY220 pKa = 9.95 GYY222 pKa = 9.31 PIQYY226 pKa = 9.27 MDD228 pKa = 4.34 VNLDD232 pKa = 3.65 DD233 pKa = 6.06 SDD235 pKa = 5.69 IEE237 pKa = 4.29 DD238 pKa = 3.65 KK239 pKa = 11.42 TNIFYY244 pKa = 10.62 EE245 pKa = 4.21 YY246 pKa = 10.82 ADD248 pKa = 4.29 YY249 pKa = 8.64 DD250 pKa = 4.13 TATCASDD257 pKa = 4.01 DD258 pKa = 3.99 ACSSRR263 pKa = 11.84 PEE265 pKa = 4.3 SAWLLRR271 pKa = 11.84 EE272 pKa = 4.08 YY273 pKa = 10.62 EE274 pKa = 4.27 VV275 pKa = 3.97
Molecular weight: 30.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.63
IPC_protein 3.681
Toseland 3.439
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.35
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 4.101
Thurlkill 3.503
EMBOSS 3.656
Sillero 3.808
Patrickios 0.82
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A3F3QET8|A0A3F3QET8_9EURO MFS general substrate transporter OS=Aspergillus welwitschiae OX=1341132 GN=BDQ94DRAFT_136924 PE=3 SV=1
MM1 pKa = 7.64 RR2 pKa = 11.84 HH3 pKa = 5.24 NPQRR7 pKa = 11.84 GEE9 pKa = 3.95 TRR11 pKa = 11.84 RR12 pKa = 11.84 LRR14 pKa = 11.84 AQGWRR19 pKa = 11.84 QGIASGGSVIGAWRR33 pKa = 11.84 AGQSARR39 pKa = 11.84 PWVSIFHH46 pKa = 7.11 AKK48 pKa = 8.52 TASQVRR54 pKa = 11.84 RR55 pKa = 3.61
Molecular weight: 6.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.389
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.115
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.149
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13680
0
13680
6103230
49
7054
446.1
49.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.374 ± 0.022
1.383 ± 0.009
5.534 ± 0.014
6.007 ± 0.017
3.763 ± 0.011
6.757 ± 0.017
2.504 ± 0.01
5.013 ± 0.014
4.358 ± 0.016
9.238 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.212 ± 0.008
3.578 ± 0.012
6.015 ± 0.021
4.009 ± 0.013
6.164 ± 0.019
8.346 ± 0.026
6.02 ± 0.015
6.253 ± 0.015
1.526 ± 0.007
2.945 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here