Cacao Bacilliform SriLanka Virus
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4U9A0|A0A2H4U9A0_9VIRU ORF2 protein OS=Cacao Bacilliform SriLanka Virus OX=2056878 PE=4 SV=1
MM1 pKa = 7.44 SGSSSSSRR9 pKa = 11.84 IIATKK14 pKa = 10.02 SYY16 pKa = 10.66 KK17 pKa = 10.24 DD18 pKa = 4.03 AILASDD24 pKa = 4.17 EE25 pKa = 4.11 VDD27 pKa = 3.61 PPAVGFAKK35 pKa = 10.45 ASEE38 pKa = 4.34 GTASNLATIVKK49 pKa = 9.59 QNNLIIALLTVVAEE63 pKa = 4.89 RR64 pKa = 11.84 ITEE67 pKa = 4.01 KK68 pKa = 10.38 EE69 pKa = 4.02 LPIVEE74 pKa = 4.33 SSSAGLEE81 pKa = 3.97 EE82 pKa = 4.67 ALDD85 pKa = 4.69 EE86 pKa = 4.75 ITDD89 pKa = 3.48 QLRR92 pKa = 11.84 NLKK95 pKa = 10.09 LRR97 pKa = 11.84 STGKK101 pKa = 10.2 EE102 pKa = 3.62 PVITEE107 pKa = 4.41 TKK109 pKa = 9.76 GKK111 pKa = 10.48 LYY113 pKa = 10.69 VFKK116 pKa = 10.92 DD117 pKa = 3.15 PLQILKK123 pKa = 10.3 EE124 pKa = 4.18 EE125 pKa = 4.14 KK126 pKa = 10.26 EE127 pKa = 4.03 KK128 pKa = 11.22 AKK130 pKa = 10.65 RR131 pKa = 3.46
Molecular weight: 14.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.545
IPC2_protein 5.741
IPC_protein 5.575
Toseland 5.448
ProMoST 5.652
Dawson 5.486
Bjellqvist 5.626
Wikipedia 5.308
Rodwell 5.423
Grimsley 5.359
Solomon 5.486
Lehninger 5.436
Nozaki 5.588
DTASelect 5.677
Thurlkill 5.423
EMBOSS 5.334
Sillero 5.677
Patrickios 4.978
IPC_peptide 5.499
IPC2_peptide 5.652
IPC2.peptide.svr19 6.039
Protein with the highest isoelectric point:
>tr|A0A2H4U997|A0A2H4U997_9VIRU Reverse transcriptase OS=Cacao Bacilliform SriLanka Virus OX=2056878 PE=4 SV=1
MM1 pKa = 7.29 SARR4 pKa = 11.84 RR5 pKa = 11.84 SDD7 pKa = 3.44 TGSSSEE13 pKa = 4.08 ANQGSSANDD22 pKa = 3.47 ADD24 pKa = 4.5 PGFSTTQEE32 pKa = 3.63 LWEE35 pKa = 4.29 LLKK38 pKa = 10.43 PSKK41 pKa = 9.97 PITMSEE47 pKa = 3.89 DD48 pKa = 2.9 LSNRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 FTEE57 pKa = 3.48 IQKK60 pKa = 10.01 RR61 pKa = 11.84 AKK63 pKa = 9.51 EE64 pKa = 3.81 RR65 pKa = 11.84 AKK67 pKa = 10.85 AAVEE71 pKa = 4.27 SALSEE76 pKa = 4.29 YY77 pKa = 10.82 EE78 pKa = 4.45 GILSIRR84 pKa = 11.84 RR85 pKa = 11.84 EE86 pKa = 4.1 ATRR89 pKa = 11.84 QLATNDD95 pKa = 3.76 NAWGDD100 pKa = 3.88 VLPLRR105 pKa = 11.84 VEE107 pKa = 4.73 EE108 pKa = 3.74 IRR110 pKa = 11.84 EE111 pKa = 4.01 IKK113 pKa = 9.57 RR114 pKa = 11.84 AKK116 pKa = 9.74 HH117 pKa = 5.97 DD118 pKa = 4.42 LYY120 pKa = 11.41 LALDD124 pKa = 3.56 KK125 pKa = 10.52 STQYY129 pKa = 11.43 AHH131 pKa = 7.51 LYY133 pKa = 9.28
Molecular weight: 15.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.569
IPC2_protein 6.338
IPC_protein 7.102
Toseland 7.571
ProMoST 7.541
Dawson 7.278
Bjellqvist 6.751
Wikipedia 7.293
Rodwell 7.19
Grimsley 7.351
Solomon 7.717
Lehninger 7.702
Nozaki 7.102
DTASelect 7.41
Thurlkill 7.439
EMBOSS 7.673
Sillero 7.468
Patrickios 4.851
IPC_peptide 7.717
IPC2_peptide 6.927
IPC2.peptide.svr19 6.949
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2179
131
1772
544.8
62.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.828 ± 1.794
1.377 ± 0.658
4.956 ± 0.63
9.087 ± 1.037
3.488 ± 0.959
5.232 ± 1.068
1.79 ± 0.425
6.792 ± 0.396
7.664 ± 0.69
9.087 ± 1.528
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.249 ± 0.596
4.497 ± 0.51
4.727 ± 0.625
5.507 ± 0.957
6.241 ± 0.609
6.884 ± 1.808
5.048 ± 0.491
5.002 ± 0.478
1.377 ± 0.327
3.167 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here