Mycobacterium phage Bxz1 (Mycobacteriophage Bxz1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bixzunavirus; Mycobacterium virus Bxz1

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q853F1|Q853F1_BPMBZ Uncharacterized protein OS=Mycobacterium phage Bxz1 OX=205877 GN=130 PE=4 SV=1
MM1 pKa = 7.81ADD3 pKa = 3.58YY4 pKa = 11.13ASDD7 pKa = 4.1CPISRR12 pKa = 11.84PVDD15 pKa = 3.83PGWTSDD21 pKa = 2.87WDD23 pKa = 3.89LGGMQWSYY31 pKa = 11.65DD32 pKa = 3.36YY33 pKa = 11.9GMFYY37 pKa = 10.44QPSPDD42 pKa = 3.22EE43 pKa = 4.38LLPLRR48 pKa = 11.84KK49 pKa = 8.64PAQRR53 pKa = 11.84VGEE56 pKa = 4.11

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q853L8|Q853L8_BPMBZ Uncharacterized protein OS=Mycobacterium phage Bxz1 OX=205877 GN=59 PE=4 SV=1
MM1 pKa = 7.42EE2 pKa = 5.71LVLFDD7 pKa = 4.87EE8 pKa = 5.23LTPQQQEE15 pKa = 3.6AWLHH19 pKa = 6.76DD20 pKa = 3.8EE21 pKa = 4.71ALDD24 pKa = 3.8MAHH27 pKa = 6.5RR28 pKa = 11.84WLRR31 pKa = 11.84GRR33 pKa = 11.84RR34 pKa = 11.84QRR36 pKa = 11.84EE37 pKa = 3.96MLAKK41 pKa = 9.38MYY43 pKa = 10.16RR44 pKa = 11.84HH45 pKa = 5.2QRR47 pKa = 11.84VYY49 pKa = 10.95GPRR52 pKa = 11.84GSRR55 pKa = 11.84WGYY58 pKa = 9.07SADD61 pKa = 3.33PRR63 pKa = 11.84RR64 pKa = 11.84ILIALLRR71 pKa = 11.84RR72 pKa = 11.84DD73 pKa = 4.43LVIKK77 pKa = 10.18CDD79 pKa = 3.69SAVPPPRR86 pKa = 11.84PGLQLWRR93 pKa = 11.84LSDD96 pKa = 3.32MGRR99 pKa = 11.84RR100 pKa = 11.84TAKK103 pKa = 10.28IIVEE107 pKa = 4.25EE108 pKa = 4.18KK109 pKa = 10.75LL110 pKa = 3.38

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

225

0

225

46957

31

1370

208.7

23.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.79 ± 0.218

0.845 ± 0.076

7.07 ± 0.179

6.476 ± 0.194

3.092 ± 0.115

8.007 ± 0.292

2.494 ± 0.165

4.289 ± 0.124

3.806 ± 0.126

7.537 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.106

3.399 ± 0.122

5.727 ± 0.181

4.136 ± 0.125

6.879 ± 0.194

5.46 ± 0.163

6.414 ± 0.189

7.124 ± 0.193

1.885 ± 0.087

3.058 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski