Thermoproteus tenax spherical virus 1
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q647D5|Q647D5_9VIRU Uncharacterized protein OS=Thermoproteus tenax spherical virus 1 OX=292639 PE=4 SV=1
MM1 pKa = 7.24 FFYY4 pKa = 10.34 MLRR7 pKa = 11.84 HH8 pKa = 6.45 LDD10 pKa = 3.56 LALDD14 pKa = 4.16 MIAVFGLALILLSYY28 pKa = 10.44 IMPVSHH34 pKa = 6.85 MNDD37 pKa = 3.27 IIAGTIAVVAIDD49 pKa = 4.07 LVVSLGMPSDD59 pKa = 3.7 AVEE62 pKa = 4.22 DD63 pKa = 4.04 AFALIAMLAAVFGFYY78 pKa = 10.16 PPLMYY83 pKa = 10.21 MIALLIKK90 pKa = 10.28 AYY92 pKa = 10.13 DD93 pKa = 3.26 IHH95 pKa = 7.55 EE96 pKa = 4.51 DD97 pKa = 3.57 AKK99 pKa = 10.31 GLKK102 pKa = 10.2 YY103 pKa = 10.54 III105 pKa = 4.73
Molecular weight: 11.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.208
IPC2_protein 4.469
IPC_protein 4.342
Toseland 4.151
ProMoST 4.533
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.329
Rodwell 4.19
Grimsley 4.062
Solomon 4.342
Lehninger 4.304
Nozaki 4.482
DTASelect 4.762
Thurlkill 4.215
EMBOSS 4.342
Sillero 4.482
Patrickios 0.896
IPC_peptide 4.342
IPC2_peptide 4.457
IPC2.peptide.svr19 4.358
Protein with the highest isoelectric point:
>tr|Q647D1|Q647D1_9VIRU Uncharacterized protein OS=Thermoproteus tenax spherical virus 1 OX=292639 PE=4 SV=1
MM1 pKa = 7.05 EE2 pKa = 4.87 RR3 pKa = 11.84 AMRR6 pKa = 11.84 EE7 pKa = 3.32 IALAIVKK14 pKa = 8.87 TIEE17 pKa = 3.95 GARR20 pKa = 11.84 GRR22 pKa = 11.84 TVCFRR27 pKa = 11.84 IRR29 pKa = 11.84 GLKK32 pKa = 9.87 RR33 pKa = 11.84 YY34 pKa = 10.17 AEE36 pKa = 3.97 ISGVGILLVHH46 pKa = 6.54 EE47 pKa = 4.67 FFKK50 pKa = 11.02 AIEE53 pKa = 3.88 PHH55 pKa = 6.84 AVFTKK60 pKa = 10.5 AGSGKK65 pKa = 10.1 RR66 pKa = 11.84 KK67 pKa = 9.81 IIVCVPRR74 pKa = 11.84 EE75 pKa = 4.0 VVEE78 pKa = 5.56 GYY80 pKa = 10.84 LEE82 pKa = 4.31 TYY84 pKa = 9.79 RR85 pKa = 11.84 KK86 pKa = 10.21 SGVEE90 pKa = 3.78 GLIQVLWPRR99 pKa = 11.84 KK100 pKa = 9.02
Molecular weight: 11.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.228
IPC2_protein 9.385
IPC_protein 9.545
Toseland 10.306
ProMoST 9.97
Dawson 10.438
Bjellqvist 10.087
Wikipedia 10.584
Rodwell 10.847
Grimsley 10.482
Solomon 10.482
Lehninger 10.452
Nozaki 10.306
DTASelect 10.072
Thurlkill 10.306
EMBOSS 10.687
Sillero 10.35
Patrickios 10.599
IPC_peptide 10.482
IPC2_peptide 8.858
IPC2.peptide.svr19 8.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
38
0
38
6154
63
504
161.9
17.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.669 ± 0.723
1.219 ± 0.207
3.477 ± 0.389
3.884 ± 0.585
3.542 ± 0.252
7.231 ± 0.444
1.479 ± 0.182
8.076 ± 0.454
4.127 ± 0.483
9.539 ± 0.727
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.022 ± 0.231
3.331 ± 0.264
5.086 ± 0.378
2.21 ± 0.309
3.9 ± 0.508
5.704 ± 0.478
6.711 ± 0.721
9.669 ± 0.433
1.706 ± 0.179
6.321 ± 0.452
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here