Lebombo virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5QLZ7|W5QLZ7_9REOV RNA-directed RNA polymerase OS=Lebombo virus OX=40057 PE=3 SV=1
MM1 pKa = 7.29AQEE4 pKa = 3.93QVQVKK9 pKa = 10.12RR10 pKa = 11.84KK11 pKa = 6.29FTRR14 pKa = 11.84TICVLDD20 pKa = 4.26PGAKK24 pKa = 8.56TFCGKK29 pKa = 8.83VAKK32 pKa = 10.7LEE34 pKa = 4.23GKK36 pKa = 8.31TYY38 pKa = 10.32FVIKK42 pKa = 10.24IGRR45 pKa = 11.84TTQVGVSNTPVPKK58 pKa = 10.38CYY60 pKa = 10.56VLDD63 pKa = 3.72VAGPGSYY70 pKa = 9.06RR71 pKa = 11.84VQDD74 pKa = 3.8GQDD77 pKa = 3.18TVSVILSEE85 pKa = 4.49SGFEE89 pKa = 4.01ATGEE93 pKa = 3.84RR94 pKa = 11.84WEE96 pKa = 4.01EE97 pKa = 3.52WRR99 pKa = 11.84FEE101 pKa = 4.21VLSATPIVARR111 pKa = 11.84MAVGEE116 pKa = 4.59DD117 pKa = 3.48EE118 pKa = 4.68FDD120 pKa = 4.63AEE122 pKa = 4.27IKK124 pKa = 10.1YY125 pKa = 10.88SKK127 pKa = 10.81GLGSVPPYY135 pKa = 10.79AKK137 pKa = 10.62NEE139 pKa = 3.91ADD141 pKa = 3.6RR142 pKa = 11.84RR143 pKa = 11.84MMPTLPGVKK152 pKa = 9.91ALDD155 pKa = 3.33MPIRR159 pKa = 11.84DD160 pKa = 3.98FRR162 pKa = 11.84QHH164 pKa = 5.8ARR166 pKa = 11.84EE167 pKa = 3.95HH168 pKa = 6.32RR169 pKa = 11.84DD170 pKa = 3.21EE171 pKa = 4.82VRR173 pKa = 11.84AQLDD177 pKa = 3.37RR178 pKa = 11.84AARR181 pKa = 11.84RR182 pKa = 11.84LDD184 pKa = 3.68PSSSRR189 pKa = 11.84LLGASVGLSEE199 pKa = 5.38EE200 pKa = 4.09IPIEE204 pKa = 4.22SVLPQAKK211 pKa = 9.63RR212 pKa = 11.84EE213 pKa = 4.1PVVFASASRR222 pKa = 11.84EE223 pKa = 3.81LDD225 pKa = 2.96DD226 pKa = 4.87WGFDD230 pKa = 3.73MQPKK234 pKa = 9.62TSGDD238 pKa = 3.27WGAKK242 pKa = 7.51TPIPEE247 pKa = 4.15VEE249 pKa = 4.07SDD251 pKa = 3.34EE252 pKa = 5.92GEE254 pKa = 4.25MNDD257 pKa = 3.53THH259 pKa = 6.3ITTAFISKK267 pKa = 10.05CSEE270 pKa = 3.67AAKK273 pKa = 10.22KK274 pKa = 10.14HH275 pKa = 4.83STTFSGLSARR285 pKa = 11.84VPAASGKK292 pKa = 8.93FDD294 pKa = 3.8EE295 pKa = 5.27VILVKK300 pKa = 10.55KK301 pKa = 9.48SAWGDD306 pKa = 3.49VPLFRR311 pKa = 11.84IDD313 pKa = 2.93EE314 pKa = 4.2ATRR317 pKa = 11.84KK318 pKa = 9.83FEE320 pKa = 4.01FMAIGTSTQVLCVKK334 pKa = 10.72DD335 pKa = 3.94DD336 pKa = 4.03LSYY339 pKa = 10.88MLLPTAHH346 pKa = 7.07

Molecular weight: 38.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5QLZ0|W5QLZ0_9REOV Outer capsid protein VP2 OS=Lebombo virus OX=40057 PE=3 SV=1
MM1 pKa = 7.79EE2 pKa = 5.12EE3 pKa = 4.32RR4 pKa = 11.84SQEE7 pKa = 4.03TPLTRR12 pKa = 11.84EE13 pKa = 3.77EE14 pKa = 4.24EE15 pKa = 4.32RR16 pKa = 11.84IKK18 pKa = 10.17MLRR21 pKa = 11.84RR22 pKa = 11.84EE23 pKa = 3.95VDD25 pKa = 2.98RR26 pKa = 11.84LLNLQIGRR34 pKa = 11.84NWEE37 pKa = 4.2AGTDD41 pKa = 3.28WMLAQIDD48 pKa = 3.96VLLFRR53 pKa = 11.84IRR55 pKa = 11.84VGNIKK60 pKa = 9.83QAEE63 pKa = 4.08EE64 pKa = 3.77QLLGMRR70 pKa = 11.84DD71 pKa = 3.53RR72 pKa = 11.84LEE74 pKa = 4.35DD75 pKa = 3.41ALEE78 pKa = 4.03RR79 pKa = 11.84KK80 pKa = 9.16RR81 pKa = 11.84RR82 pKa = 11.84GWRR85 pKa = 11.84VVWPPRR91 pKa = 11.84RR92 pKa = 3.51

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

6275

92

1298

570.5

64.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.299 ± 0.555

1.004 ± 0.268

6.167 ± 0.253

6.741 ± 0.401

3.952 ± 0.316

6.279 ± 0.411

2.327 ± 0.258

5.833 ± 0.275

5.546 ± 0.442

9.004 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.474 ± 0.194

3.618 ± 0.271

4.335 ± 0.356

3.283 ± 0.344

7.363 ± 0.368

6.406 ± 0.449

5.243 ± 0.338

7.251 ± 0.184

1.386 ± 0.27

3.49 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski