Bole Tick Virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Alpharicinrhavirus; Bole alpharicinrhavirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KTD4|A0A0B5KTD4_9RHAB GDP polyribonucleotidyltransferase OS=Bole Tick Virus 2 OX=1608041 GN=L PE=4 SV=1
MM1 pKa = 6.77GTVGEE6 pKa = 4.39VFLLLSLLSFLPLAGSDD23 pKa = 3.65PVVKK27 pKa = 10.56AIAAPYY33 pKa = 8.57FFPEE37 pKa = 3.82NLNYY41 pKa = 10.0AWHH44 pKa = 6.99PIEE47 pKa = 4.53VTSLTCPPQRR57 pKa = 11.84SIPEE61 pKa = 3.84QDD63 pKa = 2.92NGIPVIFEE71 pKa = 4.44TIHH74 pKa = 6.0PSSLEE79 pKa = 3.74RR80 pKa = 11.84ALVNGYY86 pKa = 9.5SCYY89 pKa = 9.8TSTMAVKK96 pKa = 10.5CSVNFVGWKK105 pKa = 7.95TLSHH109 pKa = 6.44QITNKK114 pKa = 9.68EE115 pKa = 4.06PSSTTCWEE123 pKa = 4.28AIKK126 pKa = 10.37RR127 pKa = 11.84QEE129 pKa = 5.28DD130 pKa = 4.44GAQSPPPTFPAPNCAWWSEE149 pKa = 3.65NWAEE153 pKa = 4.46LDD155 pKa = 3.44YY156 pKa = 11.35TLVLKK161 pKa = 10.83HH162 pKa = 6.54PARR165 pKa = 11.84QDD167 pKa = 3.26PYY169 pKa = 11.13TEE171 pKa = 4.22ALYY174 pKa = 10.88DD175 pKa = 3.69PLFPGGSCNKK185 pKa = 9.87AEE187 pKa = 4.98CPLIHH192 pKa = 7.71DD193 pKa = 4.44GGIWIQTEE201 pKa = 4.2PLASICKK208 pKa = 9.35HH209 pKa = 4.86WEE211 pKa = 3.72VLQGLTYY218 pKa = 9.75TGPEE222 pKa = 3.32IGRR225 pKa = 11.84ILFSPEE231 pKa = 3.93GPPKK235 pKa = 10.7YY236 pKa = 9.76LDD238 pKa = 3.31HH239 pKa = 6.71SCRR242 pKa = 11.84MTFCGRR248 pKa = 11.84RR249 pKa = 11.84GYY251 pKa = 10.6RR252 pKa = 11.84LQDD255 pKa = 3.23GEE257 pKa = 4.59FLVFSSPPAWGVPPVCPAGTLVRR280 pKa = 11.84AHH282 pKa = 5.98TPEE285 pKa = 4.31EE286 pKa = 3.96EE287 pKa = 4.18IRR289 pKa = 11.84WNEE292 pKa = 3.45ISKK295 pKa = 9.48MEE297 pKa = 4.06EE298 pKa = 3.9ADD300 pKa = 3.97RR301 pKa = 11.84LMCISRR307 pKa = 11.84LSVAYY312 pKa = 8.41ATGKK316 pKa = 10.52VSLEE320 pKa = 3.95LLGSLVPSHH329 pKa = 6.75GGPGTAYY336 pKa = 10.41RR337 pKa = 11.84INNGTLEE344 pKa = 4.32AAHH347 pKa = 6.05VKK349 pKa = 9.47YY350 pKa = 10.88VPLINSSNEE359 pKa = 3.57EE360 pKa = 3.52GDD362 pKa = 4.28LIGVGPDD369 pKa = 3.2GTPILWEE376 pKa = 3.88YY377 pKa = 9.65WVLSGSRR384 pKa = 11.84IIGPNGVYY392 pKa = 9.96KK393 pKa = 10.65SKK395 pKa = 10.81GRR397 pKa = 11.84IIVPNFEE404 pKa = 4.24RR405 pKa = 11.84RR406 pKa = 11.84KK407 pKa = 8.79LTYY410 pKa = 10.5DD411 pKa = 3.3LTIHH415 pKa = 5.87VFEE418 pKa = 5.08DD419 pKa = 3.72LKK421 pKa = 10.98EE422 pKa = 4.22IPHH425 pKa = 6.9PSLVIRR431 pKa = 11.84SNHH434 pKa = 5.09TDD436 pKa = 3.37LLRR439 pKa = 11.84KK440 pKa = 9.35VSHH443 pKa = 5.67NQGVEE448 pKa = 3.77GDD450 pKa = 3.26HH451 pKa = 6.35WASIRR456 pKa = 11.84LWFSSLWGSFIWTCGLALIGLILICCICRR485 pKa = 11.84RR486 pKa = 11.84VRR488 pKa = 11.84CCCRR492 pKa = 11.84GCGRR496 pKa = 11.84PQSKK500 pKa = 9.59EE501 pKa = 3.59AGGWEE506 pKa = 4.29SIEE509 pKa = 4.13MNDD512 pKa = 4.26LL513 pKa = 3.69

Molecular weight: 57.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KRE4|A0A0B5KRE4_9RHAB Glycoprotein OS=Bole Tick Virus 2 OX=1608041 GN=G PE=4 SV=1
MM1 pKa = 7.12QYY3 pKa = 10.53SWTKK7 pKa = 11.18DD8 pKa = 3.22LMFLFSNPLIFIFRR22 pKa = 11.84PILHH26 pKa = 7.0EE27 pKa = 4.47KK28 pKa = 8.75NQQISITMWRR38 pKa = 11.84FWRR41 pKa = 11.84VKK43 pKa = 10.24SPQPPPIPVEE53 pKa = 4.06EE54 pKa = 4.43EE55 pKa = 3.89EE56 pKa = 4.47EE57 pKa = 4.03EE58 pKa = 4.54DD59 pKa = 4.64LFGQIPSAPRR69 pKa = 11.84PKK71 pKa = 10.49GLRR74 pKa = 11.84ALLQGWRR81 pKa = 11.84ARR83 pKa = 11.84TRR85 pKa = 11.84STRR88 pKa = 11.84PSVHH92 pKa = 6.35ARR94 pKa = 11.84PDD96 pKa = 3.37LWAHH100 pKa = 6.66PLDD103 pKa = 4.96DD104 pKa = 6.17LPNTWTGLAVPNLPSEE120 pKa = 4.41KK121 pKa = 9.42PEE123 pKa = 4.23HH124 pKa = 5.45VTLDD128 pKa = 3.05IAATLEE134 pKa = 4.0VRR136 pKa = 11.84APRR139 pKa = 11.84PLDD142 pKa = 3.34QKK144 pKa = 9.67TLAEE148 pKa = 4.07MSLNFPLAYY157 pKa = 9.95QGEE160 pKa = 4.53ARR162 pKa = 11.84SKK164 pKa = 10.41PLFSVLLAHH173 pKa = 6.92LVSKK177 pKa = 11.02ASAHH181 pKa = 4.18QTRR184 pKa = 11.84SGLIYY189 pKa = 10.32SASLTEE195 pKa = 4.4CVIFTGRR202 pKa = 11.84KK203 pKa = 8.86GFRR206 pKa = 11.84PDD208 pKa = 3.73NRR210 pKa = 11.84LQTYY214 pKa = 9.54QDD216 pKa = 3.42TYY218 pKa = 7.21EE219 pKa = 3.75WRR221 pKa = 11.84YY222 pKa = 9.48KK223 pKa = 10.38GQVFFWKK230 pKa = 10.27LRR232 pKa = 11.84FEE234 pKa = 4.22AHH236 pKa = 5.43PTVMKK241 pKa = 10.18GIPLHH246 pKa = 6.14QILSVEE252 pKa = 4.1VMPLLRR258 pKa = 11.84ALGLHH263 pKa = 4.62PHH265 pKa = 5.67FRR267 pKa = 11.84KK268 pKa = 10.22EE269 pKa = 3.85EE270 pKa = 3.91QYY272 pKa = 11.53LEE274 pKa = 4.12IEE276 pKa = 4.92GPQQ279 pKa = 2.99

Molecular weight: 32.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3703

255

2171

740.6

83.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.076 ± 0.707

1.512 ± 0.56

4.807 ± 0.562

6.427 ± 0.366

4.537 ± 0.578

6.562 ± 0.569

2.565 ± 0.207

5.752 ± 0.353

4.861 ± 0.42

10.883 ± 0.619

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.284

2.863 ± 0.303

6.67 ± 0.835

3.646 ± 0.391

6.184 ± 0.368

7.426 ± 0.686

5.806 ± 0.823

6.103 ± 0.454

2.079 ± 0.363

3.052 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski