Sewage-associated circular DNA molecule-1

Taxonomy: Viruses; Satellites; DNA satellites

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL58|A0A0A7CL58_9VIRU Replication-associated protein OS=Sewage-associated circular DNA molecule-1 OX=1592211 PE=4 SV=1
MM1 pKa = 7.31MMMDD5 pKa = 4.24WSPLKK10 pKa = 10.88DD11 pKa = 3.72PLEE14 pKa = 4.44DD15 pKa = 3.45LQPPKK20 pKa = 10.71KK21 pKa = 9.89SVKK24 pKa = 9.8RR25 pKa = 11.84KK26 pKa = 9.69ARR28 pKa = 11.84FHH30 pKa = 6.92ADD32 pKa = 3.25EE33 pKa = 4.98LTDD36 pKa = 5.87DD37 pKa = 5.58DD38 pKa = 6.4DD39 pKa = 6.99DD40 pKa = 4.21DD41 pKa = 4.03TTTVVRR47 pKa = 11.84TAPTPVGRR55 pKa = 11.84GQGNKK60 pKa = 8.61SSRR63 pKa = 11.84WCFTYY68 pKa = 10.84NNPSMDD74 pKa = 3.15GDD76 pKa = 3.85EE77 pKa = 4.37FVSLLEE83 pKa = 4.17SKK85 pKa = 11.04GDD87 pKa = 2.93IKK89 pKa = 10.75MAVFQKK95 pKa = 9.39EE96 pKa = 4.13TGKK99 pKa = 10.81EE100 pKa = 4.22GTEE103 pKa = 3.9HH104 pKa = 5.69FQGYY108 pKa = 7.99MEE110 pKa = 4.51TKK112 pKa = 9.99KK113 pKa = 10.76RR114 pKa = 11.84MYY116 pKa = 7.99TTGAHH121 pKa = 6.62AMLNPIRR128 pKa = 11.84MHH130 pKa = 6.74LEE132 pKa = 3.6HH133 pKa = 7.32AKK135 pKa = 8.84GTKK138 pKa = 7.68QQNEE142 pKa = 4.53KK143 pKa = 10.72YY144 pKa = 8.22CTKK147 pKa = 10.64EE148 pKa = 3.57EE149 pKa = 4.15GRR151 pKa = 11.84AGGPWYY157 pKa = 10.56ANCTTDD163 pKa = 4.0DD164 pKa = 4.12FKK166 pKa = 11.3RR167 pKa = 11.84KK168 pKa = 9.59SGNQGKK174 pKa = 10.14RR175 pKa = 11.84SDD177 pKa = 3.85LDD179 pKa = 3.16EE180 pKa = 4.39FARR183 pKa = 11.84MIKK186 pKa = 10.23EE187 pKa = 3.83EE188 pKa = 4.19GGITEE193 pKa = 4.84DD194 pKa = 3.83VFEE197 pKa = 4.96AMPGHH202 pKa = 6.55AMAYY206 pKa = 9.42SKK208 pKa = 10.51HH209 pKa = 5.84AKK211 pKa = 10.23ALVAEE216 pKa = 4.6MKK218 pKa = 9.64LTEE221 pKa = 4.01IKK223 pKa = 10.14KK224 pKa = 10.45KK225 pKa = 10.55EE226 pKa = 3.57MAYY229 pKa = 9.11WQEE232 pKa = 3.84QYY234 pKa = 9.58QRR236 pKa = 11.84RR237 pKa = 11.84QRR239 pKa = 11.84GEE241 pKa = 4.05EE242 pKa = 3.76IEE244 pKa = 4.43GQQQRR249 pKa = 11.84NLKK252 pKa = 10.43LYY254 pKa = 9.71FGPTASS260 pKa = 3.62

Molecular weight: 29.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL58|A0A0A7CL58_9VIRU Replication-associated protein OS=Sewage-associated circular DNA molecule-1 OX=1592211 PE=4 SV=1
MM1 pKa = 7.31MMMDD5 pKa = 4.24WSPLKK10 pKa = 10.88DD11 pKa = 3.72PLEE14 pKa = 4.44DD15 pKa = 3.45LQPPKK20 pKa = 10.71KK21 pKa = 9.89SVKK24 pKa = 9.8RR25 pKa = 11.84KK26 pKa = 9.69ARR28 pKa = 11.84FHH30 pKa = 6.92ADD32 pKa = 3.25EE33 pKa = 4.98LTDD36 pKa = 5.87DD37 pKa = 5.58DD38 pKa = 6.4DD39 pKa = 6.99DD40 pKa = 4.21DD41 pKa = 4.03TTTVVRR47 pKa = 11.84TAPTPVGRR55 pKa = 11.84GQGNKK60 pKa = 8.61SSRR63 pKa = 11.84WCFTYY68 pKa = 10.84NNPSMDD74 pKa = 3.15GDD76 pKa = 3.85EE77 pKa = 4.37FVSLLEE83 pKa = 4.17SKK85 pKa = 11.04GDD87 pKa = 2.93IKK89 pKa = 10.75MAVFQKK95 pKa = 9.39EE96 pKa = 4.13TGKK99 pKa = 10.81EE100 pKa = 4.22GTEE103 pKa = 3.9HH104 pKa = 5.69FQGYY108 pKa = 7.99MEE110 pKa = 4.51TKK112 pKa = 9.99KK113 pKa = 10.76RR114 pKa = 11.84MYY116 pKa = 7.99TTGAHH121 pKa = 6.62AMLNPIRR128 pKa = 11.84MHH130 pKa = 6.74LEE132 pKa = 3.6HH133 pKa = 7.32AKK135 pKa = 8.84GTKK138 pKa = 7.68QQNEE142 pKa = 4.53KK143 pKa = 10.72YY144 pKa = 8.22CTKK147 pKa = 10.64EE148 pKa = 3.57EE149 pKa = 4.15GRR151 pKa = 11.84AGGPWYY157 pKa = 10.56ANCTTDD163 pKa = 4.0DD164 pKa = 4.12FKK166 pKa = 11.3RR167 pKa = 11.84KK168 pKa = 9.59SGNQGKK174 pKa = 10.14RR175 pKa = 11.84SDD177 pKa = 3.85LDD179 pKa = 3.16EE180 pKa = 4.39FARR183 pKa = 11.84MIKK186 pKa = 10.23EE187 pKa = 3.83EE188 pKa = 4.19GGITEE193 pKa = 4.84DD194 pKa = 3.83VFEE197 pKa = 4.96AMPGHH202 pKa = 6.55AMAYY206 pKa = 9.42SKK208 pKa = 10.51HH209 pKa = 5.84AKK211 pKa = 10.23ALVAEE216 pKa = 4.6MKK218 pKa = 9.64LTEE221 pKa = 4.01IKK223 pKa = 10.14KK224 pKa = 10.45KK225 pKa = 10.55EE226 pKa = 3.57MAYY229 pKa = 9.11WQEE232 pKa = 3.84QYY234 pKa = 9.58QRR236 pKa = 11.84RR237 pKa = 11.84QRR239 pKa = 11.84GEE241 pKa = 4.05EE242 pKa = 3.76IEE244 pKa = 4.43GQQQRR249 pKa = 11.84NLKK252 pKa = 10.43LYY254 pKa = 9.71FGPTASS260 pKa = 3.62

Molecular weight: 29.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

260

260

260

260.0

29.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.923 ± 0.0

1.154 ± 0.0

6.923 ± 0.0

9.231 ± 0.0

3.462 ± 0.0

8.077 ± 0.0

2.692 ± 0.0

2.308 ± 0.0

10.385 ± 0.0

5.0 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

5.769 ± 0.0

3.077 ± 0.0

4.231 ± 0.0

5.385 ± 0.0

5.769 ± 0.0

4.231 ± 0.0

7.308 ± 0.0

3.077 ± 0.0

1.538 ± 0.0

3.462 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski