Amino acid dipepetide frequency for Rhodobacteraceae bacterium HTCC2150

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.86AlaAla: 12.86 ± 0.159	1.022AlaCys: 1.022 ± 0.035	6.524AlaAsp: 6.524 ± 0.092	6.785AlaGlu: 6.785 ± 0.097	4.14AlaPhe: 4.14 ± 0.068	8.608AlaGly: 8.608 ± 0.106	2.03AlaHis: 2.03 ± 0.051	6.336AlaIle: 6.336 ± 0.088	5.158AlaLys: 5.158 ± 0.079	11.116AlaLeu: 11.116 ± 0.126	3.468AlaMet: 3.468 ± 0.068	3.704AlaAsn: 3.704 ± 0.058	4.289AlaPro: 4.289 ± 0.079	3.791AlaGln: 3.791 ± 0.068	5.86AlaArg: 5.86 ± 0.101	5.689AlaSer: 5.689 ± 0.072	5.822AlaThr: 5.822 ± 0.089	7.119AlaVal: 7.119 ± 0.075	1.15AlaTrp: 1.15 ± 0.038	2.47AlaTyr: 2.47 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.027CysAla: 1.027 ± 0.029	0.103CysCys: 0.103 ± 0.01	0.637CysAsp: 0.637 ± 0.027	0.515CysGlu: 0.515 ± 0.023	0.43CysPhe: 0.43 ± 0.022	0.971CysGly: 0.971 ± 0.036	0.27CysHis: 0.27 ± 0.017	0.503CysIle: 0.503 ± 0.022	0.322CysLys: 0.322 ± 0.02	0.889CysLeu: 0.889 ± 0.029	0.191CysMet: 0.191 ± 0.014	0.304CysAsn: 0.304 ± 0.018	0.512CysPro: 0.512 ± 0.022	0.297CysGln: 0.297 ± 0.015	0.467CysArg: 0.467 ± 0.024	0.549CysSer: 0.549 ± 0.025	0.47CysThr: 0.47 ± 0.022	0.668CysVal: 0.668 ± 0.027	0.119CysTrp: 0.119 ± 0.01	0.239CysTyr: 0.239 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	6.653AspAla: 6.653 ± 0.096	0.558AspCys: 0.558 ± 0.022	3.669AspAsp: 3.669 ± 0.094	3.834AspGlu: 3.834 ± 0.061	2.721AspPhe: 2.721 ± 0.053	5.137AspGly: 5.137 ± 0.103	1.437AspHis: 1.437 ± 0.045	3.426AspIle: 3.426 ± 0.057	2.332AspLys: 2.332 ± 0.049	6.219AspLeu: 6.219 ± 0.104	1.731AspMet: 1.731 ± 0.042	1.706AspAsn: 1.706 ± 0.048	3.016AspPro: 3.016 ± 0.056	2.414AspGln: 2.414 ± 0.047	3.457AspArg: 3.457 ± 0.064	2.265AspSer: 2.265 ± 0.058	3.128AspThr: 3.128 ± 0.073	4.733AspVal: 4.733 ± 0.08	1.039AspTrp: 1.039 ± 0.031	1.623AspTyr: 1.623 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	6.063GluAla: 6.063 ± 0.089	0.443GluCys: 0.443 ± 0.021	2.89GluAsp: 2.89 ± 0.056	3.12GluGlu: 3.12 ± 0.082	2.437GluPhe: 2.437 ± 0.054	4.243GluGly: 4.243 ± 0.067	1.204GluHis: 1.204 ± 0.04	4.253GluIle: 4.253 ± 0.075	2.891GluLys: 2.891 ± 0.061	5.304GluLeu: 5.304 ± 0.08	2.038GluMet: 2.038 ± 0.049	2.808GluAsn: 2.808 ± 0.058	2.088GluPro: 2.088 ± 0.063	1.999GluGln: 1.999 ± 0.043	3.389GluArg: 3.389 ± 0.072	2.345GluSer: 2.345 ± 0.046	3.954GluThr: 3.954 ± 0.067	4.065GluVal: 4.065 ± 0.067	0.781GluTrp: 0.781 ± 0.03	1.243GluTyr: 1.243 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.502PheAla: 4.502 ± 0.071	0.519PheCys: 0.519 ± 0.025	3.12PheAsp: 3.12 ± 0.063	2.62PheGlu: 2.62 ± 0.05	1.739PhePhe: 1.739 ± 0.051	4.018PheGly: 4.018 ± 0.067	0.802PheHis: 0.802 ± 0.029	2.141PheIle: 2.141 ± 0.048	1.452PheLys: 1.452 ± 0.041	3.762PheLeu: 3.762 ± 0.069	1.069PheMet: 1.069 ± 0.033	1.4PheAsn: 1.4 ± 0.038	1.646PhePro: 1.646 ± 0.042	1.374PheGln: 1.374 ± 0.034	1.937PheArg: 1.937 ± 0.046	2.73PheSer: 2.73 ± 0.059	2.296PheThr: 2.296 ± 0.048	3.02PheVal: 3.02 ± 0.055	0.633PheTrp: 0.633 ± 0.026	1.13PheTyr: 1.13 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	8.266GlyAla: 8.266 ± 0.099	0.867GlyCys: 0.867 ± 0.028	4.765GlyAsp: 4.765 ± 0.112	4.534GlyGlu: 4.534 ± 0.074	3.897GlyPhe: 3.897 ± 0.062	6.733GlyGly: 6.733 ± 0.121	1.875GlyHis: 1.875 ± 0.042	4.791GlyIle: 4.791 ± 0.083	3.837GlyLys: 3.837 ± 0.056	8.114GlyLeu: 8.114 ± 0.089	2.488GlyMet: 2.488 ± 0.045	2.662GlyAsn: 2.662 ± 0.064	2.971GlyPro: 2.971 ± 0.056	3.178GlyGln: 3.178 ± 0.061	4.529GlyArg: 4.529 ± 0.069	4.276GlySer: 4.276 ± 0.092	4.436GlyThr: 4.436 ± 0.101	6.138GlyVal: 6.138 ± 0.086	1.336GlyTrp: 1.336 ± 0.042	2.215GlyTyr: 2.215 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.047HisAla: 2.047 ± 0.048	0.244HisCys: 0.244 ± 0.015	1.255HisAsp: 1.255 ± 0.033	1.086HisGlu: 1.086 ± 0.032	0.926HisPhe: 0.926 ± 0.028	1.781HisGly: 1.781 ± 0.049	0.583HisHis: 0.583 ± 0.029	1.153HisIle: 1.153 ± 0.038	0.788HisLys: 0.788 ± 0.027	2.073HisLeu: 2.073 ± 0.053	0.607HisMet: 0.607 ± 0.024	0.641HisAsn: 0.641 ± 0.022	1.198HisPro: 1.198 ± 0.035	0.666HisGln: 0.666 ± 0.026	1.148HisArg: 1.148 ± 0.036	1.12HisSer: 1.12 ± 0.032	0.941HisThr: 0.941 ± 0.028	1.437HisVal: 1.437 ± 0.038	0.338HisTrp: 0.338 ± 0.015	0.542HisTyr: 0.542 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.83IleAla: 6.83 ± 0.083	0.716IleCys: 0.716 ± 0.025	4.067IleAsp: 4.067 ± 0.065	3.775IleGlu: 3.775 ± 0.058	2.356IlePhe: 2.356 ± 0.059	5.184IleGly: 5.184 ± 0.088	1.092IleHis: 1.092 ± 0.034	3.165IleIle: 3.165 ± 0.059	2.563IleLys: 2.563 ± 0.05	5.453IleLeu: 5.453 ± 0.087	1.407IleMet: 1.407 ± 0.039	2.158IleAsn: 2.158 ± 0.048	2.526IlePro: 2.526 ± 0.056	1.567IleGln: 1.567 ± 0.044	2.936IleArg: 2.936 ± 0.052	4.086IleSer: 4.086 ± 0.065	3.43IleThr: 3.43 ± 0.064	4.152IleVal: 4.152 ± 0.057	0.824IleTrp: 0.824 ± 0.03	1.411IleTyr: 1.411 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	4.413LysAla: 4.413 ± 0.067	0.309LysCys: 0.309 ± 0.016	2.381LysAsp: 2.381 ± 0.048	2.226LysGlu: 2.226 ± 0.052	1.607LysPhe: 1.607 ± 0.043	3.453LysGly: 3.453 ± 0.058	0.849LysHis: 0.849 ± 0.023	2.834LysIle: 2.834 ± 0.063	2.108LysLys: 2.108 ± 0.05	4.071LysLeu: 4.071 ± 0.066	1.451LysMet: 1.451 ± 0.037	1.702LysAsn: 1.702 ± 0.039	2.146LysPro: 2.146 ± 0.052	1.352LysGln: 1.352 ± 0.034	2.648LysArg: 2.648 ± 0.062	2.98LysSer: 2.98 ± 0.057	2.83LysThr: 2.83 ± 0.046	2.876LysVal: 2.876 ± 0.057	0.572LysTrp: 0.572 ± 0.023	0.963LysTyr: 0.963 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	11.25LeuAla: 11.25 ± 0.133	0.98LeuCys: 0.98 ± 0.034	5.826LeuAsp: 5.826 ± 0.092	4.99LeuGlu: 4.99 ± 0.075	3.754LeuPhe: 3.754 ± 0.076	8.0LeuGly: 8.0 ± 0.094	1.702LeuHis: 1.702 ± 0.043	5.84LeuIle: 5.84 ± 0.1	4.073LeuLys: 4.073 ± 0.061	8.23LeuLeu: 8.23 ± 0.121	2.808LeuMet: 2.808 ± 0.059	3.65LeuAsn: 3.65 ± 0.063	4.803LeuPro: 4.803 ± 0.065	2.735LeuGln: 2.735 ± 0.051	5.483LeuArg: 5.483 ± 0.073	6.711LeuSer: 6.711 ± 0.086	5.695LeuThr: 5.695 ± 0.075	6.448LeuVal: 6.448 ± 0.084	1.155LeuTrp: 1.155 ± 0.036	1.995LeuTyr: 1.995 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	3.415MetAla: 3.415 ± 0.07	0.228MetCys: 0.228 ± 0.015	1.536MetAsp: 1.536 ± 0.04	1.411MetGlu: 1.411 ± 0.037	1.022MetPhe: 1.022 ± 0.029	2.467MetGly: 2.467 ± 0.056	0.519MetHis: 0.519 ± 0.023	1.831MetIle: 1.831 ± 0.04	1.366MetLys: 1.366 ± 0.041	2.597MetLeu: 2.597 ± 0.057	0.912MetMet: 0.912 ± 0.031	1.243MetAsn: 1.243 ± 0.037	1.493MetPro: 1.493 ± 0.038	1.044MetGln: 1.044 ± 0.03	1.783MetArg: 1.783 ± 0.048	1.934MetSer: 1.934 ± 0.047	1.979MetThr: 1.979 ± 0.042	2.016MetVal: 2.016 ± 0.045	0.265MetTrp: 0.265 ± 0.017	0.433MetTyr: 0.433 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	4.001AsnAla: 4.001 ± 0.063	0.399AsnCys: 0.399 ± 0.02	2.286AsnAsp: 2.286 ± 0.058	1.809AsnGlu: 1.809 ± 0.042	1.507AsnPhe: 1.507 ± 0.036	3.304AsnGly: 3.304 ± 0.069	0.743AsnHis: 0.743 ± 0.025	2.083AsnIle: 2.083 ± 0.046	1.364AsnLys: 1.364 ± 0.035	3.439AsnLeu: 3.439 ± 0.061	0.989AsnMet: 0.989 ± 0.034	1.254AsnAsn: 1.254 ± 0.038	2.181AsnPro: 2.181 ± 0.048	1.199AsnGln: 1.199 ± 0.036	1.896AsnArg: 1.896 ± 0.043	1.958AsnSer: 1.958 ± 0.048	1.829AsnThr: 1.829 ± 0.041	2.467AsnVal: 2.467 ± 0.052	0.611AsnTrp: 0.611 ± 0.025	0.895AsnTyr: 0.895 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	4.229ProAla: 4.229 ± 0.073	0.341ProCys: 0.341 ± 0.019	3.18ProAsp: 3.18 ± 0.058	3.327ProGlu: 3.327 ± 0.066	2.05ProPhe: 2.05 ± 0.048	2.872ProGly: 2.872 ± 0.055	0.954ProHis: 0.954 ± 0.035	2.752ProIle: 2.752 ± 0.05	2.366ProLys: 2.366 ± 0.058	4.045ProLeu: 4.045 ± 0.066	1.353ProMet: 1.353 ± 0.034	1.838ProAsn: 1.838 ± 0.04	1.593ProPro: 1.593 ± 0.046	1.418ProGln: 1.418 ± 0.036	2.049ProArg: 2.049 ± 0.043	2.572ProSer: 2.572 ± 0.052	2.506ProThr: 2.506 ± 0.056	3.456ProVal: 3.456 ± 0.056	0.57ProTrp: 0.57 ± 0.026	1.08ProTyr: 1.08 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.499GlnAla: 3.499 ± 0.061	0.264GlnCys: 0.264 ± 0.017	1.902GlnAsp: 1.902 ± 0.043	1.711GlnGlu: 1.711 ± 0.048	1.292GlnPhe: 1.292 ± 0.032	2.567GlnGly: 2.567 ± 0.055	0.652GlnHis: 0.652 ± 0.026	2.276GlnIle: 2.276 ± 0.046	1.603GlnLys: 1.603 ± 0.042	2.967GlnLeu: 2.967 ± 0.058	1.183GlnMet: 1.183 ± 0.035	1.485GlnAsn: 1.485 ± 0.044	1.399GlnPro: 1.399 ± 0.035	1.096GlnGln: 1.096 ± 0.033	1.94GlnArg: 1.94 ± 0.043	2.313GlnSer: 2.313 ± 0.048	2.007GlnThr: 2.007 ± 0.046	2.182GlnVal: 2.182 ± 0.05	0.414GlnTrp: 0.414 ± 0.018	0.684GlnTyr: 0.684 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	5.733ArgAla: 5.733 ± 0.094	0.443ArgCys: 0.443 ± 0.018	3.475ArgAsp: 3.475 ± 0.06	3.201ArgGlu: 3.201 ± 0.063	2.362ArgPhe: 2.362 ± 0.049	3.648ArgGly: 3.648 ± 0.063	1.199ArgHis: 1.199 ± 0.031	3.29ArgIle: 3.29 ± 0.055	2.52ArgLys: 2.52 ± 0.058	5.733ArgLeu: 5.733 ± 0.085	1.686ArgMet: 1.686 ± 0.042	1.925ArgAsn: 1.925 ± 0.049	2.42ArgPro: 2.42 ± 0.049	2.062ArgGln: 2.062 ± 0.044	3.579ArgArg: 3.579 ± 0.065	2.883ArgSer: 2.883 ± 0.057	2.541ArgThr: 2.541 ± 0.046	3.939ArgVal: 3.939 ± 0.063	0.738ArgTrp: 0.738 ± 0.023	1.331ArgTyr: 1.331 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	5.879SerAla: 5.879 ± 0.093	0.501SerCys: 0.501 ± 0.022	3.677SerAsp: 3.677 ± 0.064	3.262SerGlu: 3.262 ± 0.06	2.636SerPhe: 2.636 ± 0.055	5.52SerGly: 5.52 ± 0.092	1.216SerHis: 1.216 ± 0.034	3.499SerIle: 3.499 ± 0.061	2.476SerLys: 2.476 ± 0.055	5.566SerLeu: 5.566 ± 0.087	1.66SerMet: 1.66 ± 0.038	2.08SerAsn: 2.08 ± 0.049	2.468SerPro: 2.468 ± 0.045	1.827SerGln: 1.827 ± 0.037	2.943SerArg: 2.943 ± 0.053	3.156SerSer: 3.156 ± 0.062	2.913SerThr: 2.913 ± 0.059	4.082SerVal: 4.082 ± 0.071	0.757SerTrp: 0.757 ± 0.026	1.424SerTyr: 1.424 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	5.841ThrAla: 5.841 ± 0.092	0.513ThrCys: 0.513 ± 0.023	3.365ThrAsp: 3.365 ± 0.06	2.968ThrGlu: 2.968 ± 0.06	2.196ThrPhe: 2.196 ± 0.047	5.003ThrGly: 5.003 ± 0.086	1.168ThrHis: 1.168 ± 0.035	3.279ThrIle: 3.279 ± 0.064	2.345ThrLys: 2.345 ± 0.05	5.798ThrLeu: 5.798 ± 0.096	1.463ThrMet: 1.463 ± 0.039	1.927ThrAsn: 1.927 ± 0.042	3.087ThrPro: 3.087 ± 0.06	1.838ThrGln: 1.838 ± 0.048	2.866ThrArg: 2.866 ± 0.049	3.286ThrSer: 3.286 ± 0.062	3.167ThrThr: 3.167 ± 0.07	3.95ThrVal: 3.95 ± 0.08	0.679ThrTrp: 0.679 ± 0.027	1.377ThrTyr: 1.377 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	7.769ValAla: 7.769 ± 0.083	0.625ValCys: 0.625 ± 0.027	4.233ValAsp: 4.233 ± 0.081	4.306ValGlu: 4.306 ± 0.067	3.112ValPhe: 3.112 ± 0.06	5.249ValGly: 5.249 ± 0.084	1.328ValHis: 1.328 ± 0.04	4.364ValIle: 4.364 ± 0.063	2.826ValLys: 2.826 ± 0.058	6.94ValLeu: 6.94 ± 0.092	2.062ValMet: 2.062 ± 0.048	2.464ValAsn: 2.464 ± 0.051	3.07ValPro: 3.07 ± 0.054	2.134ValGln: 2.134 ± 0.05	3.477ValArg: 3.477 ± 0.064	4.502ValSer: 4.502 ± 0.07	4.332ValThr: 4.332 ± 0.068	5.413ValVal: 5.413 ± 0.086	0.938ValTrp: 0.938 ± 0.033	1.568ValTyr: 1.568 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.342TrpAla: 1.342 ± 0.039	0.138TrpCys: 0.138 ± 0.011	0.726TrpAsp: 0.726 ± 0.024	0.639TrpGlu: 0.639 ± 0.024	0.619TrpPhe: 0.619 ± 0.025	1.017TrpGly: 1.017 ± 0.039	0.378TrpHis: 0.378 ± 0.019	0.759TrpIle: 0.759 ± 0.027	0.514TrpLys: 0.514 ± 0.026	1.456TrpLeu: 1.456 ± 0.04	0.406TrpMet: 0.406 ± 0.02	0.503TrpAsn: 0.503 ± 0.021	0.625TrpPro: 0.625 ± 0.028	0.587TrpGln: 0.587 ± 0.027	0.91TrpArg: 0.91 ± 0.031	0.755TrpSer: 0.755 ± 0.024	0.673TrpThr: 0.673 ± 0.025	0.976TrpVal: 0.976 ± 0.032	0.219TrpTrp: 0.219 ± 0.013	0.286TrpTyr: 0.286 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.319TyrAla: 2.319 ± 0.047	0.272TyrCys: 0.272 ± 0.014	1.588TyrAsp: 1.588 ± 0.043	1.439TyrGlu: 1.439 ± 0.044	1.107TyrPhe: 1.107 ± 0.034	2.132TyrGly: 2.132 ± 0.049	0.575TyrHis: 0.575 ± 0.025	1.122TyrIle: 1.122 ± 0.035	0.867TyrLys: 0.867 ± 0.028	2.353TyrLeu: 2.353 ± 0.046	0.519TyrMet: 0.519 ± 0.022	0.758TyrAsn: 0.758 ± 0.031	1.021TyrPro: 1.021 ± 0.032	0.859TyrGln: 0.859 ± 0.03	1.414TyrArg: 1.414 ± 0.041	1.433TyrSer: 1.433 ± 0.042	1.167TyrThr: 1.167 ± 0.038	1.575TyrVal: 1.575 ± 0.035	0.39TyrTrp: 0.39 ± 0.019	0.725TyrTyr: 0.725 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3636 proteins (1082448 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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