Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides fragilis

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5LG53|Q5LG53_BACFN Putative N utilization substance protein OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=BF9343_1107 PE=3 SV=1
MM1 pKa = 7.28EE2 pKa = 5.61AVTLSEE8 pKa = 3.54ARR10 pKa = 11.84VYY12 pKa = 10.88VGTYY16 pKa = 9.56NKK18 pKa = 10.08YY19 pKa = 10.6NNGSLFGKK27 pKa = 9.26WLDD30 pKa = 3.72LSDD33 pKa = 5.06YY34 pKa = 10.62SDD36 pKa = 3.6KK37 pKa = 11.68DD38 pKa = 3.47EE39 pKa = 4.84FMEE42 pKa = 5.13ACRR45 pKa = 11.84EE46 pKa = 4.05LHH48 pKa = 7.22KK49 pKa = 10.91DD50 pKa = 3.54DD51 pKa = 4.58QDD53 pKa = 3.96PEE55 pKa = 5.08FMFQDD60 pKa = 3.9YY61 pKa = 11.28EE62 pKa = 4.42NIPEE66 pKa = 4.13ALISEE71 pKa = 4.48SWLSEE76 pKa = 3.76KK77 pKa = 10.49FFEE80 pKa = 5.55LRR82 pKa = 11.84DD83 pKa = 4.29AIEE86 pKa = 4.77KK87 pKa = 10.64LSEE90 pKa = 4.12TQQEE94 pKa = 4.99AFFVWCDD101 pKa = 3.32HH102 pKa = 6.55HH103 pKa = 8.25NSDD106 pKa = 5.34ISEE109 pKa = 4.05EE110 pKa = 4.1DD111 pKa = 3.42ADD113 pKa = 4.39DD114 pKa = 5.21LISSFEE120 pKa = 4.1DD121 pKa = 4.17EE122 pKa = 4.47YY123 pKa = 11.31QGEE126 pKa = 4.44YY127 pKa = 10.22KK128 pKa = 10.73DD129 pKa = 4.14EE130 pKa = 3.79EE131 pKa = 5.05DD132 pKa = 3.37YY133 pKa = 11.08AYY135 pKa = 10.08EE136 pKa = 4.47IVEE139 pKa = 3.94QCYY142 pKa = 10.53DD143 pKa = 3.45LPEE146 pKa = 4.03FAKK149 pKa = 10.18TYY151 pKa = 10.45FDD153 pKa = 3.79YY154 pKa = 11.39SAFARR159 pKa = 11.84DD160 pKa = 5.17LFITDD165 pKa = 2.97YY166 pKa = 11.18WMDD169 pKa = 3.15NGFVFRR175 pKa = 11.84CAA177 pKa = 3.58

Molecular weight: 21.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5LF76|Q5LF76_BACFN Uncharacterized protein OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=BF9343_1441 PE=4 SV=1
MM1 pKa = 7.91PSGKK5 pKa = 9.66KK6 pKa = 9.7KK7 pKa = 9.82KK8 pKa = 7.49RR9 pKa = 11.84HH10 pKa = 5.6KK11 pKa = 10.32MSTHH15 pKa = 5.04KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.87KK19 pKa = 8.77RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.28NRR25 pKa = 11.84HH26 pKa = 4.94KK27 pKa = 10.86SKK29 pKa = 10.75KK30 pKa = 9.6

Molecular weight: 3.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4234

0

4234

1537139

30

1957

363.0

41.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.887 ± 0.034

1.231 ± 0.015

5.408 ± 0.025

6.51 ± 0.037

4.632 ± 0.027

6.864 ± 0.034

1.83 ± 0.015

6.999 ± 0.037

6.622 ± 0.032

9.279 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.017

5.047 ± 0.03

3.793 ± 0.018

3.446 ± 0.02

4.719 ± 0.029

6.172 ± 0.032

5.61 ± 0.023

6.479 ± 0.029

1.264 ± 0.014

4.49 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski