Amino acid dipepetide frequency for Streptococcus phage Spn1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.742AlaAla: 2.742 ± 0.767
0.392AlaCys: 0.392 ± 0.214
5.954AlaAsp: 5.954 ± 0.56
6.111AlaGlu: 6.111 ± 0.683
2.429AlaPhe: 2.429 ± 0.678
4.779AlaGly: 4.779 ± 1.008
0.783AlaHis: 0.783 ± 0.293
4.701AlaIle: 4.701 ± 0.882
6.111AlaLys: 6.111 ± 0.793
5.798AlaLeu: 5.798 ± 0.817
2.037AlaMet: 2.037 ± 0.432
3.839AlaAsn: 3.839 ± 0.738
2.115AlaPro: 2.115 ± 0.398
1.88AlaGln: 1.88 ± 0.262
2.977AlaArg: 2.977 ± 0.626
2.429AlaSer: 2.429 ± 0.552
4.936AlaThr: 4.936 ± 0.905
5.406AlaVal: 5.406 ± 0.962
1.175AlaTrp: 1.175 ± 0.468
1.645AlaTyr: 1.645 ± 0.331
0.0AlaXaa: 0.0 ± 0.0
Cys
0.235CysAla: 0.235 ± 0.116
0.078CysCys: 0.078 ± 0.09
0.313CysAsp: 0.313 ± 0.134
0.392CysGlu: 0.392 ± 0.153
0.392CysPhe: 0.392 ± 0.161
0.157CysGly: 0.157 ± 0.113
0.0CysHis: 0.0 ± 0.0
0.313CysIle: 0.313 ± 0.205
0.705CysLys: 0.705 ± 0.221
0.548CysLeu: 0.548 ± 0.204
0.0CysMet: 0.0 ± 0.0
0.157CysAsn: 0.157 ± 0.179
0.313CysPro: 0.313 ± 0.165
0.235CysGln: 0.235 ± 0.137
0.392CysArg: 0.392 ± 0.157
0.235CysSer: 0.235 ± 0.142
0.078CysThr: 0.078 ± 0.072
0.235CysVal: 0.235 ± 0.123
0.157CysTrp: 0.157 ± 0.1
0.392CysTyr: 0.392 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
3.447AspAla: 3.447 ± 0.706
0.548AspCys: 0.548 ± 0.225
3.604AspAsp: 3.604 ± 0.776
5.092AspGlu: 5.092 ± 0.982
2.742AspPhe: 2.742 ± 0.443
4.701AspGly: 4.701 ± 0.643
0.548AspHis: 0.548 ± 0.209
5.484AspIle: 5.484 ± 0.555
5.406AspLys: 5.406 ± 0.857
5.249AspLeu: 5.249 ± 0.673
1.41AspMet: 1.41 ± 0.335
2.742AspAsn: 2.742 ± 0.448
1.645AspPro: 1.645 ± 0.319
1.88AspGln: 1.88 ± 0.346
2.429AspArg: 2.429 ± 0.496
3.604AspSer: 3.604 ± 0.568
3.291AspThr: 3.291 ± 0.405
3.917AspVal: 3.917 ± 0.525
1.41AspTrp: 1.41 ± 0.306
2.585AspTyr: 2.585 ± 0.386
0.0AspXaa: 0.0 ± 0.0
Glu
6.424GluAla: 6.424 ± 0.716
0.078GluCys: 0.078 ± 0.068
3.839GluAsp: 3.839 ± 0.612
6.189GluGlu: 6.189 ± 1.09
3.761GluPhe: 3.761 ± 0.583
3.996GluGly: 3.996 ± 0.593
1.41GluHis: 1.41 ± 0.368
6.189GluIle: 6.189 ± 0.843
7.286GluLys: 7.286 ± 1.266
7.835GluLeu: 7.835 ± 0.992
1.959GluMet: 1.959 ± 0.434
4.074GluAsn: 4.074 ± 0.585
1.175GluPro: 1.175 ± 0.302
2.977GluGln: 2.977 ± 0.571
3.839GluArg: 3.839 ± 0.612
5.014GluSer: 5.014 ± 0.625
4.152GluThr: 4.152 ± 0.524
5.641GluVal: 5.641 ± 0.751
1.254GluTrp: 1.254 ± 0.291
2.585GluTyr: 2.585 ± 0.556
0.0GluXaa: 0.0 ± 0.0
Phe
2.35PheAla: 2.35 ± 0.58
0.235PheCys: 0.235 ± 0.137
3.996PheAsp: 3.996 ± 0.571
3.369PheGlu: 3.369 ± 0.508
1.88PhePhe: 1.88 ± 0.376
2.507PheGly: 2.507 ± 0.658
0.235PheHis: 0.235 ± 0.137
2.194PheIle: 2.194 ± 0.458
3.369PheLys: 3.369 ± 0.411
2.585PheLeu: 2.585 ± 0.413
0.94PheMet: 0.94 ± 0.314
2.585PheAsn: 2.585 ± 0.5
0.705PhePro: 0.705 ± 0.236
1.332PheGln: 1.332 ± 0.32
1.88PheArg: 1.88 ± 0.271
3.134PheSer: 3.134 ± 0.752
2.585PheThr: 2.585 ± 0.372
1.802PheVal: 1.802 ± 0.399
0.783PheTrp: 0.783 ± 0.249
1.88PheTyr: 1.88 ± 0.403
0.0PheXaa: 0.0 ± 0.0
Gly
3.055GlyAla: 3.055 ± 0.559
0.157GlyCys: 0.157 ± 0.109
3.917GlyAsp: 3.917 ± 0.738
4.622GlyGlu: 4.622 ± 0.68
3.134GlyPhe: 3.134 ± 0.665
4.857GlyGly: 4.857 ± 1.287
1.018GlyHis: 1.018 ± 0.235
3.604GlyIle: 3.604 ± 0.65
5.484GlyLys: 5.484 ± 0.622
6.111GlyLeu: 6.111 ± 1.0
1.645GlyMet: 1.645 ± 0.285
3.761GlyAsn: 3.761 ± 0.527
1.018GlyPro: 1.018 ± 0.281
3.447GlyGln: 3.447 ± 0.567
3.682GlyArg: 3.682 ± 0.624
3.604GlySer: 3.604 ± 0.947
2.664GlyThr: 2.664 ± 0.5
4.309GlyVal: 4.309 ± 0.779
1.018GlyTrp: 1.018 ± 0.392
2.742GlyTyr: 2.742 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
0.862HisAla: 0.862 ± 0.276
0.0HisCys: 0.0 ± 0.0
0.783HisAsp: 0.783 ± 0.319
1.332HisGlu: 1.332 ± 0.327
0.627HisPhe: 0.627 ± 0.223
0.862HisGly: 0.862 ± 0.33
0.392HisHis: 0.392 ± 0.193
0.862HisIle: 0.862 ± 0.277
0.783HisLys: 0.783 ± 0.266
1.175HisLeu: 1.175 ± 0.369
0.157HisMet: 0.157 ± 0.114
1.175HisAsn: 1.175 ± 0.307
0.862HisPro: 0.862 ± 0.278
0.627HisGln: 0.627 ± 0.228
0.862HisArg: 0.862 ± 0.258
1.567HisSer: 1.567 ± 0.419
0.862HisThr: 0.862 ± 0.274
0.705HisVal: 0.705 ± 0.242
0.078HisTrp: 0.078 ± 0.09
0.862HisTyr: 0.862 ± 0.292
0.0HisXaa: 0.0 ± 0.0
Ile
5.327IleAla: 5.327 ± 0.836
0.47IleCys: 0.47 ± 0.164
3.447IleAsp: 3.447 ± 0.574
6.816IleGlu: 6.816 ± 0.889
2.82IlePhe: 2.82 ± 0.586
5.092IleGly: 5.092 ± 0.799
0.783IleHis: 0.783 ± 0.349
3.055IleIle: 3.055 ± 0.586
6.503IleLys: 6.503 ± 0.719
4.387IleLeu: 4.387 ± 0.66
1.489IleMet: 1.489 ± 0.327
3.761IleAsn: 3.761 ± 0.576
1.41IlePro: 1.41 ± 0.335
2.429IleGln: 2.429 ± 0.339
2.82IleArg: 2.82 ± 0.484
5.406IleSer: 5.406 ± 0.857
4.387IleThr: 4.387 ± 0.537
4.074IleVal: 4.074 ± 0.662
0.705IleTrp: 0.705 ± 0.271
2.507IleTyr: 2.507 ± 0.656
0.0IleXaa: 0.0 ± 0.0
Lys
5.563LysAla: 5.563 ± 0.621
0.392LysCys: 0.392 ± 0.188
5.876LysAsp: 5.876 ± 0.621
7.599LysGlu: 7.599 ± 1.033
3.291LysPhe: 3.291 ± 0.577
4.466LysGly: 4.466 ± 0.661
1.802LysHis: 1.802 ± 0.343
6.268LysIle: 6.268 ± 0.815
7.521LysLys: 7.521 ± 1.082
7.991LysLeu: 7.991 ± 0.702
2.664LysMet: 2.664 ± 0.47
3.604LysAsn: 3.604 ± 0.531
2.194LysPro: 2.194 ± 0.56
3.604LysGln: 3.604 ± 0.593
3.996LysArg: 3.996 ± 0.608
4.074LysSer: 4.074 ± 0.418
5.954LysThr: 5.954 ± 0.597
5.954LysVal: 5.954 ± 0.767
0.94LysTrp: 0.94 ± 0.252
3.291LysTyr: 3.291 ± 0.412
0.0LysXaa: 0.0 ± 0.0
Leu
6.973LeuAla: 6.973 ± 0.872
0.47LeuCys: 0.47 ± 0.314
5.327LeuAsp: 5.327 ± 0.704
7.208LeuGlu: 7.208 ± 0.905
2.585LeuPhe: 2.585 ± 0.47
5.327LeuGly: 5.327 ± 1.467
1.175LeuHis: 1.175 ± 0.31
4.074LeuIle: 4.074 ± 0.541
7.678LeuLys: 7.678 ± 0.937
6.659LeuLeu: 6.659 ± 0.927
2.194LeuMet: 2.194 ± 0.413
3.761LeuAsn: 3.761 ± 0.474
3.447LeuPro: 3.447 ± 0.541
3.604LeuGln: 3.604 ± 0.637
3.682LeuArg: 3.682 ± 0.489
4.779LeuSer: 4.779 ± 0.646
6.033LeuThr: 6.033 ± 0.756
4.152LeuVal: 4.152 ± 0.502
1.018LeuTrp: 1.018 ± 0.21
2.35LeuTyr: 2.35 ± 0.385
0.0LeuXaa: 0.0 ± 0.0
Met
1.88MetAla: 1.88 ± 0.393
0.0MetCys: 0.0 ± 0.0
1.41MetAsp: 1.41 ± 0.319
1.88MetGlu: 1.88 ± 0.465
0.94MetPhe: 0.94 ± 0.291
1.254MetGly: 1.254 ± 0.471
0.235MetHis: 0.235 ± 0.138
1.88MetIle: 1.88 ± 0.479
2.35MetLys: 2.35 ± 0.385
1.645MetLeu: 1.645 ± 0.353
0.47MetMet: 0.47 ± 0.186
1.645MetAsn: 1.645 ± 0.401
0.783MetPro: 0.783 ± 0.29
0.783MetGln: 0.783 ± 0.269
1.567MetArg: 1.567 ± 0.365
1.645MetSer: 1.645 ± 0.382
1.567MetThr: 1.567 ± 0.488
1.645MetVal: 1.645 ± 0.346
0.235MetTrp: 0.235 ± 0.135
0.862MetTyr: 0.862 ± 0.238
0.0MetXaa: 0.0 ± 0.0
Asn
4.466AsnAla: 4.466 ± 1.049
0.313AsnCys: 0.313 ± 0.139
2.585AsnAsp: 2.585 ± 0.542
2.977AsnGlu: 2.977 ± 0.594
1.88AsnPhe: 1.88 ± 0.432
3.996AsnGly: 3.996 ± 0.615
0.94AsnHis: 0.94 ± 0.301
3.212AsnIle: 3.212 ± 0.628
4.779AsnLys: 4.779 ± 0.609
4.231AsnLeu: 4.231 ± 0.532
1.41AsnMet: 1.41 ± 0.334
2.272AsnAsn: 2.272 ± 0.463
1.802AsnPro: 1.802 ± 0.37
3.134AsnGln: 3.134 ± 0.695
2.977AsnArg: 2.977 ± 0.565
3.604AsnSer: 3.604 ± 0.766
2.35AsnThr: 2.35 ± 0.583
2.82AsnVal: 2.82 ± 0.414
1.018AsnTrp: 1.018 ± 0.274
1.959AsnTyr: 1.959 ± 0.45
0.0AsnXaa: 0.0 ± 0.0
Pro
1.802ProAla: 1.802 ± 0.515
0.078ProCys: 0.078 ± 0.079
1.959ProAsp: 1.959 ± 0.409
3.447ProGlu: 3.447 ± 0.393
0.627ProPhe: 0.627 ± 0.264
1.175ProGly: 1.175 ± 0.231
0.313ProHis: 0.313 ± 0.123
2.429ProIle: 2.429 ± 0.461
2.664ProLys: 2.664 ± 0.455
1.332ProLeu: 1.332 ± 0.395
0.548ProMet: 0.548 ± 0.217
1.959ProAsn: 1.959 ± 0.48
0.783ProPro: 0.783 ± 0.352
0.705ProGln: 0.705 ± 0.208
1.175ProArg: 1.175 ± 0.283
1.802ProSer: 1.802 ± 0.456
0.783ProThr: 0.783 ± 0.261
1.724ProVal: 1.724 ± 0.344
0.392ProTrp: 0.392 ± 0.226
1.724ProTyr: 1.724 ± 0.469
0.0ProXaa: 0.0 ± 0.0
Gln
3.682GlnAla: 3.682 ± 0.491
0.235GlnCys: 0.235 ± 0.138
1.41GlnAsp: 1.41 ± 0.311
3.055GlnGlu: 3.055 ± 0.67
1.41GlnPhe: 1.41 ± 0.298
1.724GlnGly: 1.724 ± 0.424
0.627GlnHis: 0.627 ± 0.233
3.604GlnIle: 3.604 ± 0.501
3.761GlnLys: 3.761 ± 0.597
3.291GlnLeu: 3.291 ± 0.446
1.018GlnMet: 1.018 ± 0.24
1.645GlnAsn: 1.645 ± 0.351
1.018GlnPro: 1.018 ± 0.263
1.489GlnGln: 1.489 ± 0.358
1.724GlnArg: 1.724 ± 0.439
2.429GlnSer: 2.429 ± 0.37
2.82GlnThr: 2.82 ± 0.411
3.369GlnVal: 3.369 ± 0.549
0.47GlnTrp: 0.47 ± 0.169
0.94GlnTyr: 0.94 ± 0.318
0.0GlnXaa: 0.0 ± 0.0
Arg
2.899ArgAla: 2.899 ± 0.481
0.392ArgCys: 0.392 ± 0.165
2.272ArgAsp: 2.272 ± 0.388
3.134ArgGlu: 3.134 ± 0.641
1.802ArgPhe: 1.802 ± 0.406
2.272ArgGly: 2.272 ± 0.38
0.862ArgHis: 0.862 ± 0.287
2.82ArgIle: 2.82 ± 0.437
3.917ArgLys: 3.917 ± 0.66
5.014ArgLeu: 5.014 ± 0.789
2.272ArgMet: 2.272 ± 0.436
2.899ArgAsn: 2.899 ± 0.494
1.097ArgPro: 1.097 ± 0.246
1.88ArgGln: 1.88 ± 0.502
2.585ArgArg: 2.585 ± 0.591
2.507ArgSer: 2.507 ± 0.55
2.664ArgThr: 2.664 ± 0.509
2.35ArgVal: 2.35 ± 0.43
0.47ArgTrp: 0.47 ± 0.178
1.802ArgTyr: 1.802 ± 0.426
0.0ArgXaa: 0.0 ± 0.0
Ser
4.466SerAla: 4.466 ± 1.03
0.235SerCys: 0.235 ± 0.099
3.526SerAsp: 3.526 ± 0.453
3.839SerGlu: 3.839 ± 0.508
2.115SerPhe: 2.115 ± 0.347
4.936SerGly: 4.936 ± 0.814
1.254SerHis: 1.254 ± 0.36
4.387SerIle: 4.387 ± 0.578
4.701SerLys: 4.701 ± 0.741
5.798SerLeu: 5.798 ± 0.637
1.254SerMet: 1.254 ± 0.341
2.977SerAsn: 2.977 ± 0.599
1.489SerPro: 1.489 ± 0.325
2.507SerGln: 2.507 ± 0.36
2.664SerArg: 2.664 ± 0.615
3.996SerSer: 3.996 ± 0.879
4.231SerThr: 4.231 ± 0.729
3.447SerVal: 3.447 ± 0.722
0.94SerTrp: 0.94 ± 0.363
2.742SerTyr: 2.742 ± 0.549
0.0SerXaa: 0.0 ± 0.0
Thr
4.309ThrAla: 4.309 ± 0.959
0.235ThrCys: 0.235 ± 0.131
3.917ThrAsp: 3.917 ± 0.513
4.231ThrGlu: 4.231 ± 0.517
3.212ThrPhe: 3.212 ± 0.53
3.917ThrGly: 3.917 ± 0.728
1.097ThrHis: 1.097 ± 0.33
5.092ThrIle: 5.092 ± 0.481
4.622ThrLys: 4.622 ± 0.736
4.466ThrLeu: 4.466 ± 0.639
0.862ThrMet: 0.862 ± 0.239
3.682ThrAsn: 3.682 ± 0.446
1.802ThrPro: 1.802 ± 0.511
2.507ThrGln: 2.507 ± 0.44
1.567ThrArg: 1.567 ± 0.325
4.074ThrSer: 4.074 ± 0.712
4.701ThrThr: 4.701 ± 0.726
3.839ThrVal: 3.839 ± 0.706
0.627ThrTrp: 0.627 ± 0.257
2.664ThrTyr: 2.664 ± 0.598
0.0ThrXaa: 0.0 ± 0.0
Val
4.857ValAla: 4.857 ± 0.646
0.47ValCys: 0.47 ± 0.213
3.996ValAsp: 3.996 ± 0.631
4.936ValGlu: 4.936 ± 0.56
1.959ValPhe: 1.959 ± 0.359
4.936ValGly: 4.936 ± 0.659
0.94ValHis: 0.94 ± 0.303
4.387ValIle: 4.387 ± 0.711
5.171ValLys: 5.171 ± 0.625
4.701ValLeu: 4.701 ± 0.576
1.097ValMet: 1.097 ± 0.289
3.839ValAsn: 3.839 ± 0.843
1.88ValPro: 1.88 ± 0.359
1.567ValGln: 1.567 ± 0.41
2.35ValArg: 2.35 ± 0.326
4.779ValSer: 4.779 ± 0.633
4.309ValThr: 4.309 ± 0.631
4.387ValVal: 4.387 ± 0.838
0.47ValTrp: 0.47 ± 0.173
2.115ValTyr: 2.115 ± 0.424
0.0ValXaa: 0.0 ± 0.0
Trp
1.254TrpAla: 1.254 ± 0.402
0.157TrpCys: 0.157 ± 0.103
0.783TrpAsp: 0.783 ± 0.342
1.018TrpGlu: 1.018 ± 0.3
1.018TrpPhe: 1.018 ± 0.463
0.548TrpGly: 0.548 ± 0.192
0.078TrpHis: 0.078 ± 0.076
0.627TrpIle: 0.627 ± 0.221
1.097TrpLys: 1.097 ± 0.282
0.705TrpLeu: 0.705 ± 0.305
0.313TrpMet: 0.313 ± 0.152
1.018TrpAsn: 1.018 ± 0.316
0.078TrpPro: 0.078 ± 0.084
1.018TrpGln: 1.018 ± 0.348
0.313TrpArg: 0.313 ± 0.167
0.392TrpSer: 0.392 ± 0.176
0.94TrpThr: 0.94 ± 0.302
1.332TrpVal: 1.332 ± 0.271
0.157TrpTrp: 0.157 ± 0.108
0.705TrpTyr: 0.705 ± 0.434
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.645TyrAla: 1.645 ± 0.331
0.392TyrCys: 0.392 ± 0.166
2.82TyrAsp: 2.82 ± 0.537
2.429TyrGlu: 2.429 ± 0.515
1.724TyrPhe: 1.724 ± 0.458
2.35TyrGly: 2.35 ± 0.439
0.94TyrHis: 0.94 ± 0.303
2.507TyrIle: 2.507 ± 0.49
3.134TyrLys: 3.134 ± 0.549
2.977TyrLeu: 2.977 ± 0.487
0.862TyrMet: 0.862 ± 0.278
1.332TyrAsn: 1.332 ± 0.364
1.802TyrPro: 1.802 ± 0.383
2.115TyrGln: 2.115 ± 0.425
2.507TyrArg: 2.507 ± 0.492
2.429TyrSer: 2.429 ± 0.536
2.115TyrThr: 2.115 ± 0.318
2.037TyrVal: 2.037 ± 0.438
0.235TyrTrp: 0.235 ± 0.149
1.724TyrTyr: 1.724 ± 0.529
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12765 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski