Amino acid dipepetide frequency for Clostridium phage phiCD24-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.619AlaAla: 2.619 ± 0.931
0.524AlaCys: 0.524 ± 0.252
2.469AlaAsp: 2.469 ± 0.487
3.292AlaGlu: 3.292 ± 0.733
1.197AlaPhe: 1.197 ± 0.427
1.945AlaGly: 1.945 ± 0.405
0.524AlaHis: 0.524 ± 0.166
4.564AlaIle: 4.564 ± 1.084
4.564AlaLys: 4.564 ± 0.828
4.863AlaLeu: 4.863 ± 0.617
2.843AlaMet: 2.843 ± 1.121
2.918AlaAsn: 2.918 ± 0.47
0.299AlaPro: 0.299 ± 0.166
1.347AlaGln: 1.347 ± 0.361
2.02AlaArg: 2.02 ± 0.363
4.265AlaSer: 4.265 ± 1.129
2.993AlaThr: 2.993 ± 0.52
3.142AlaVal: 3.142 ± 0.878
0.449AlaTrp: 0.449 ± 0.19
2.095AlaTyr: 2.095 ± 0.546
0.0AlaXaa: 0.0 ± 0.0
Cys
0.449CysAla: 0.449 ± 0.183
0.224CysCys: 0.224 ± 0.166
0.599CysAsp: 0.599 ± 0.176
1.122CysGlu: 1.122 ± 0.279
0.449CysPhe: 0.449 ± 0.19
0.224CysGly: 0.224 ± 0.141
0.0CysHis: 0.0 ± 0.0
1.047CysIle: 1.047 ± 0.343
1.047CysLys: 1.047 ± 0.262
0.524CysLeu: 0.524 ± 0.25
0.075CysMet: 0.075 ± 0.075
0.673CysAsn: 0.673 ± 0.226
0.374CysPro: 0.374 ± 0.216
0.075CysGln: 0.075 ± 0.082
0.224CysArg: 0.224 ± 0.149
0.299CysSer: 0.299 ± 0.192
0.823CysThr: 0.823 ± 0.305
0.224CysVal: 0.224 ± 0.114
0.0CysTrp: 0.0 ± 0.0
0.374CysTyr: 0.374 ± 0.197
0.0CysXaa: 0.0 ± 0.0
Asp
2.693AspAla: 2.693 ± 0.663
0.374AspCys: 0.374 ± 0.162
2.544AspAsp: 2.544 ± 0.552
4.564AspGlu: 4.564 ± 0.659
2.319AspPhe: 2.319 ± 0.419
3.89AspGly: 3.89 ± 0.525
0.075AspHis: 0.075 ± 0.078
6.808AspIle: 6.808 ± 0.604
6.135AspLys: 6.135 ± 0.616
5.536AspLeu: 5.536 ± 0.504
1.796AspMet: 1.796 ± 0.485
4.19AspAsn: 4.19 ± 0.731
0.599AspPro: 0.599 ± 0.337
0.748AspGln: 0.748 ± 0.206
2.095AspArg: 2.095 ± 0.411
3.442AspSer: 3.442 ± 0.375
3.741AspThr: 3.741 ± 0.638
3.442AspVal: 3.442 ± 0.454
0.748AspTrp: 0.748 ± 0.258
2.993AspTyr: 2.993 ± 0.539
0.0AspXaa: 0.0 ± 0.0
Glu
4.04GluAla: 4.04 ± 0.647
0.449GluCys: 0.449 ± 0.248
4.04GluAsp: 4.04 ± 0.61
11.223GluGlu: 11.223 ± 1.857
3.217GluPhe: 3.217 ± 0.647
4.265GluGly: 4.265 ± 0.728
1.047GluHis: 1.047 ± 0.292
9.277GluIle: 9.277 ± 1.03
6.883GluLys: 6.883 ± 0.942
9.352GluLeu: 9.352 ± 0.926
2.095GluMet: 2.095 ± 0.453
6.659GluAsn: 6.659 ± 0.738
1.646GluPro: 1.646 ± 0.344
3.666GluGln: 3.666 ± 0.8
2.993GluArg: 2.993 ± 0.57
5.387GluSer: 5.387 ± 0.76
4.04GluThr: 4.04 ± 1.219
5.387GluVal: 5.387 ± 0.905
0.823GluTrp: 0.823 ± 0.286
2.993GluTyr: 2.993 ± 0.46
0.0GluXaa: 0.0 ± 0.0
Phe
1.047PheAla: 1.047 ± 0.292
0.524PheCys: 0.524 ± 0.211
2.319PheAsp: 2.319 ± 0.468
3.816PheGlu: 3.816 ± 0.564
0.898PhePhe: 0.898 ± 0.275
2.245PheGly: 2.245 ± 0.398
0.823PheHis: 0.823 ± 0.351
3.367PheIle: 3.367 ± 0.617
4.19PheLys: 4.19 ± 0.614
2.544PheLeu: 2.544 ± 0.507
1.047PheMet: 1.047 ± 0.353
3.591PheAsn: 3.591 ± 0.516
0.673PhePro: 0.673 ± 0.23
0.673PheGln: 0.673 ± 0.193
0.898PheArg: 0.898 ± 0.265
2.245PheSer: 2.245 ± 0.346
2.394PheThr: 2.394 ± 0.444
1.197PheVal: 1.197 ± 0.243
0.524PheTrp: 0.524 ± 0.229
1.122PheTyr: 1.122 ± 0.328
0.0PheXaa: 0.0 ± 0.0
Gly
2.768GlyAla: 2.768 ± 0.636
0.374GlyCys: 0.374 ± 0.238
2.768GlyAsp: 2.768 ± 0.529
3.816GlyGlu: 3.816 ± 0.528
1.721GlyPhe: 1.721 ± 0.293
2.245GlyGly: 2.245 ± 0.445
0.599GlyHis: 0.599 ± 0.226
3.965GlyIle: 3.965 ± 0.424
6.135GlyLys: 6.135 ± 0.997
4.19GlyLeu: 4.19 ± 0.51
1.571GlyMet: 1.571 ± 0.325
4.115GlyAsn: 4.115 ± 0.926
0.898GlyPro: 0.898 ± 0.294
2.319GlyGln: 2.319 ± 0.388
1.721GlyArg: 1.721 ± 0.35
3.591GlySer: 3.591 ± 0.455
3.442GlyThr: 3.442 ± 0.737
2.095GlyVal: 2.095 ± 0.424
0.823GlyTrp: 0.823 ± 0.259
2.619GlyTyr: 2.619 ± 0.453
0.0GlyXaa: 0.0 ± 0.0
His
0.599HisAla: 0.599 ± 0.218
0.0HisCys: 0.0 ± 0.0
0.449HisAsp: 0.449 ± 0.193
0.973HisGlu: 0.973 ± 0.256
0.673HisPhe: 0.673 ± 0.216
0.374HisGly: 0.374 ± 0.22
0.224HisHis: 0.224 ± 0.144
1.272HisIle: 1.272 ± 0.317
0.748HisLys: 0.748 ± 0.247
0.673HisLeu: 0.673 ± 0.308
0.15HisMet: 0.15 ± 0.108
0.374HisAsn: 0.374 ± 0.131
0.599HisPro: 0.599 ± 0.273
0.449HisGln: 0.449 ± 0.169
0.449HisArg: 0.449 ± 0.188
0.673HisSer: 0.673 ± 0.292
0.823HisThr: 0.823 ± 0.336
0.524HisVal: 0.524 ± 0.169
0.075HisTrp: 0.075 ± 0.076
0.224HisTyr: 0.224 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
4.938IleAla: 4.938 ± 1.175
0.973IleCys: 0.973 ± 0.24
6.06IleAsp: 6.06 ± 0.535
9.951IleGlu: 9.951 ± 0.704
3.067IlePhe: 3.067 ± 0.782
4.489IleGly: 4.489 ± 0.677
0.599IleHis: 0.599 ± 0.221
6.285IleIle: 6.285 ± 0.987
10.549IleLys: 10.549 ± 0.973
6.734IleLeu: 6.734 ± 0.855
1.047IleMet: 1.047 ± 0.297
7.033IleAsn: 7.033 ± 0.885
2.394IlePro: 2.394 ± 0.465
1.945IleGln: 1.945 ± 0.488
3.516IleArg: 3.516 ± 0.793
6.584IleSer: 6.584 ± 0.666
5.162IleThr: 5.162 ± 0.644
4.713IleVal: 4.713 ± 0.632
0.748IleTrp: 0.748 ± 0.278
3.067IleTyr: 3.067 ± 0.566
0.0IleXaa: 0.0 ± 0.0
Lys
5.237LysAla: 5.237 ± 0.67
0.673LysCys: 0.673 ± 0.237
7.257LysAsp: 7.257 ± 0.787
10.325LysGlu: 10.325 ± 1.437
3.816LysPhe: 3.816 ± 0.68
4.564LysGly: 4.564 ± 0.567
0.823LysHis: 0.823 ± 0.314
7.706LysIle: 7.706 ± 0.716
10.848LysLys: 10.848 ± 1.615
8.903LysLeu: 8.903 ± 0.88
3.142LysMet: 3.142 ± 0.45
6.883LysAsn: 6.883 ± 0.806
1.571LysPro: 1.571 ± 0.306
3.591LysGln: 3.591 ± 0.701
3.292LysArg: 3.292 ± 0.593
7.631LysSer: 7.631 ± 1.056
5.611LysThr: 5.611 ± 0.597
5.611LysVal: 5.611 ± 0.586
1.122LysTrp: 1.122 ± 0.283
4.19LysTyr: 4.19 ± 0.643
0.0LysXaa: 0.0 ± 0.0
Leu
4.265LeuAla: 4.265 ± 0.709
0.673LeuCys: 0.673 ± 0.219
5.462LeuAsp: 5.462 ± 0.553
7.257LeuGlu: 7.257 ± 0.837
2.768LeuPhe: 2.768 ± 0.449
4.339LeuGly: 4.339 ± 0.627
0.898LeuHis: 0.898 ± 0.199
6.06LeuIle: 6.06 ± 1.223
9.427LeuLys: 9.427 ± 1.001
5.611LeuLeu: 5.611 ± 1.008
1.422LeuMet: 1.422 ± 0.458
6.734LeuAsn: 6.734 ± 0.929
2.245LeuPro: 2.245 ± 0.496
3.367LeuGln: 3.367 ± 0.505
3.067LeuArg: 3.067 ± 0.506
6.285LeuSer: 6.285 ± 0.616
5.611LeuThr: 5.611 ± 0.72
3.965LeuVal: 3.965 ± 0.551
0.449LeuTrp: 0.449 ± 0.154
3.816LeuTyr: 3.816 ± 0.59
0.0LeuXaa: 0.0 ± 0.0
Met
2.095MetAla: 2.095 ± 0.704
0.299MetCys: 0.299 ± 0.142
1.197MetAsp: 1.197 ± 0.302
1.945MetGlu: 1.945 ± 0.471
1.047MetPhe: 1.047 ± 0.302
0.748MetGly: 0.748 ± 0.281
0.15MetHis: 0.15 ± 0.122
1.496MetIle: 1.496 ± 0.348
1.945MetLys: 1.945 ± 0.376
1.945MetLeu: 1.945 ± 0.412
0.524MetMet: 0.524 ± 0.184
2.469MetAsn: 2.469 ± 0.771
0.748MetPro: 0.748 ± 0.217
0.898MetGln: 0.898 ± 0.255
0.748MetArg: 0.748 ± 0.205
1.796MetSer: 1.796 ± 0.555
1.496MetThr: 1.496 ± 0.375
0.898MetVal: 0.898 ± 0.205
0.299MetTrp: 0.299 ± 0.21
1.197MetTyr: 1.197 ± 0.365
0.0MetXaa: 0.0 ± 0.0
Asn
3.067AsnAla: 3.067 ± 1.083
0.748AsnCys: 0.748 ± 0.279
3.067AsnAsp: 3.067 ± 0.5
6.659AsnGlu: 6.659 ± 1.529
2.843AsnPhe: 2.843 ± 0.47
4.713AsnGly: 4.713 ± 0.664
0.449AsnHis: 0.449 ± 0.213
6.958AsnIle: 6.958 ± 1.048
9.202AsnLys: 9.202 ± 1.042
5.611AsnLeu: 5.611 ± 0.64
1.496AsnMet: 1.496 ± 0.369
6.359AsnAsn: 6.359 ± 1.039
2.095AsnPro: 2.095 ± 0.467
2.319AsnGln: 2.319 ± 0.517
2.918AsnArg: 2.918 ± 0.461
6.584AsnSer: 6.584 ± 1.131
4.265AsnThr: 4.265 ± 0.84
3.142AsnVal: 3.142 ± 0.68
0.599AsnTrp: 0.599 ± 0.221
2.394AsnTyr: 2.394 ± 0.505
0.0AsnXaa: 0.0 ± 0.0
Pro
0.524ProAla: 0.524 ± 0.213
0.299ProCys: 0.299 ± 0.172
1.122ProAsp: 1.122 ± 0.371
1.646ProGlu: 1.646 ± 0.478
1.122ProPhe: 1.122 ± 0.248
1.047ProGly: 1.047 ± 0.341
0.374ProHis: 0.374 ± 0.166
1.646ProIle: 1.646 ± 0.364
2.319ProLys: 2.319 ± 0.571
1.122ProLeu: 1.122 ± 0.345
0.748ProMet: 0.748 ± 0.216
1.496ProAsn: 1.496 ± 0.408
0.15ProPro: 0.15 ± 0.112
0.599ProGln: 0.599 ± 0.177
0.748ProArg: 0.748 ± 0.258
1.272ProSer: 1.272 ± 0.363
1.272ProThr: 1.272 ± 0.297
1.197ProVal: 1.197 ± 0.345
0.224ProTrp: 0.224 ± 0.149
0.673ProTyr: 0.673 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
1.945GlnAla: 1.945 ± 0.447
0.224GlnCys: 0.224 ± 0.135
2.02GlnAsp: 2.02 ± 0.358
2.693GlnGlu: 2.693 ± 0.452
1.047GlnPhe: 1.047 ± 0.241
2.768GlnGly: 2.768 ± 0.472
0.599GlnHis: 0.599 ± 0.192
4.04GlnIle: 4.04 ± 0.768
2.843GlnLys: 2.843 ± 0.632
3.292GlnLeu: 3.292 ± 0.439
0.524GlnMet: 0.524 ± 0.211
1.945GlnAsn: 1.945 ± 0.346
0.374GlnPro: 0.374 ± 0.209
1.272GlnGln: 1.272 ± 0.37
0.898GlnArg: 0.898 ± 0.212
1.272GlnSer: 1.272 ± 0.263
1.796GlnThr: 1.796 ± 0.377
1.646GlnVal: 1.646 ± 0.353
0.15GlnTrp: 0.15 ± 0.084
1.496GlnTyr: 1.496 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
1.422ArgAla: 1.422 ± 0.346
0.673ArgCys: 0.673 ± 0.2
1.721ArgAsp: 1.721 ± 0.384
3.816ArgGlu: 3.816 ± 0.774
1.272ArgPhe: 1.272 ± 0.272
1.571ArgGly: 1.571 ± 0.471
0.224ArgHis: 0.224 ± 0.127
3.666ArgIle: 3.666 ± 0.585
4.339ArgLys: 4.339 ± 1.106
3.067ArgLeu: 3.067 ± 0.422
1.197ArgMet: 1.197 ± 0.27
2.394ArgAsn: 2.394 ± 0.558
0.524ArgPro: 0.524 ± 0.313
1.047ArgGln: 1.047 ± 0.284
0.823ArgArg: 0.823 ± 0.261
1.422ArgSer: 1.422 ± 0.323
1.571ArgThr: 1.571 ± 0.308
1.87ArgVal: 1.87 ± 0.289
0.374ArgTrp: 0.374 ± 0.175
1.422ArgTyr: 1.422 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
2.993SerAla: 2.993 ± 0.809
0.299SerCys: 0.299 ± 0.165
4.489SerAsp: 4.489 ± 0.798
4.713SerGlu: 4.713 ± 1.244
2.095SerPhe: 2.095 ± 0.336
3.367SerGly: 3.367 ± 0.606
1.047SerHis: 1.047 ± 0.29
5.611SerIle: 5.611 ± 0.789
7.257SerLys: 7.257 ± 0.859
5.686SerLeu: 5.686 ± 0.648
1.945SerMet: 1.945 ± 0.391
5.387SerAsn: 5.387 ± 0.533
1.347SerPro: 1.347 ± 0.27
2.693SerGln: 2.693 ± 0.629
1.796SerArg: 1.796 ± 0.33
5.985SerSer: 5.985 ± 1.879
5.536SerThr: 5.536 ± 1.067
3.442SerVal: 3.442 ± 0.489
0.898SerTrp: 0.898 ± 0.212
2.993SerTyr: 2.993 ± 0.586
0.0SerXaa: 0.0 ± 0.0
Thr
3.292ThrAla: 3.292 ± 1.067
0.299ThrCys: 0.299 ± 0.126
4.04ThrAsp: 4.04 ± 0.627
4.788ThrGlu: 4.788 ± 0.547
2.469ThrPhe: 2.469 ± 0.37
3.89ThrGly: 3.89 ± 0.559
1.122ThrHis: 1.122 ± 0.382
5.761ThrIle: 5.761 ± 0.815
4.938ThrLys: 4.938 ± 0.587
5.611ThrLeu: 5.611 ± 0.839
0.973ThrMet: 0.973 ± 0.324
4.414ThrAsn: 4.414 ± 0.831
1.047ThrPro: 1.047 ± 0.294
2.245ThrGln: 2.245 ± 0.492
2.918ThrArg: 2.918 ± 1.227
4.19ThrSer: 4.19 ± 0.648
4.339ThrThr: 4.339 ± 0.776
2.693ThrVal: 2.693 ± 0.537
0.524ThrTrp: 0.524 ± 0.188
2.319ThrTyr: 2.319 ± 0.421
0.0ThrXaa: 0.0 ± 0.0
Val
2.918ValAla: 2.918 ± 0.814
0.599ValCys: 0.599 ± 0.212
4.04ValAsp: 4.04 ± 0.606
3.292ValGlu: 3.292 ± 0.572
1.796ValPhe: 1.796 ± 0.475
2.993ValGly: 2.993 ± 0.505
0.524ValHis: 0.524 ± 0.155
5.387ValIle: 5.387 ± 0.593
3.965ValLys: 3.965 ± 0.609
3.965ValLeu: 3.965 ± 0.488
0.673ValMet: 0.673 ± 0.324
3.442ValAsn: 3.442 ± 0.345
0.823ValPro: 0.823 ± 0.313
1.496ValGln: 1.496 ± 0.3
1.646ValArg: 1.646 ± 0.454
3.89ValSer: 3.89 ± 0.442
3.367ValThr: 3.367 ± 0.494
3.217ValVal: 3.217 ± 0.531
0.075ValTrp: 0.075 ± 0.059
2.693ValTyr: 2.693 ± 0.538
0.0ValXaa: 0.0 ± 0.0
Trp
0.374TrpAla: 0.374 ± 0.23
0.15TrpCys: 0.15 ± 0.091
1.197TrpAsp: 1.197 ± 0.432
0.599TrpGlu: 0.599 ± 0.202
0.449TrpPhe: 0.449 ± 0.204
0.599TrpGly: 0.599 ± 0.215
0.075TrpHis: 0.075 ± 0.082
0.524TrpIle: 0.524 ± 0.218
0.599TrpLys: 0.599 ± 0.227
0.898TrpLeu: 0.898 ± 0.306
0.15TrpMet: 0.15 ± 0.093
0.823TrpAsn: 0.823 ± 0.412
0.0TrpPro: 0.0 ± 0.0
0.449TrpGln: 0.449 ± 0.204
0.15TrpArg: 0.15 ± 0.113
0.524TrpSer: 0.524 ± 0.212
0.599TrpThr: 0.599 ± 0.174
0.673TrpVal: 0.673 ± 0.187
0.524TrpTrp: 0.524 ± 0.361
0.449TrpTyr: 0.449 ± 0.258
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.646TyrAla: 1.646 ± 0.334
0.449TyrCys: 0.449 ± 0.22
1.87TyrAsp: 1.87 ± 0.396
2.993TyrGlu: 2.993 ± 0.502
1.87TyrPhe: 1.87 ± 0.476
1.496TyrGly: 1.496 ± 0.286
0.15TyrHis: 0.15 ± 0.099
4.414TyrIle: 4.414 ± 0.757
4.863TyrLys: 4.863 ± 0.869
3.666TyrLeu: 3.666 ± 0.555
0.449TyrMet: 0.449 ± 0.165
3.591TyrAsn: 3.591 ± 0.644
1.197TyrPro: 1.197 ± 0.31
1.646TyrGln: 1.646 ± 0.349
1.496TyrArg: 1.496 ± 0.295
2.245TyrSer: 2.245 ± 0.458
2.993TyrThr: 2.993 ± 0.597
1.721TyrVal: 1.721 ± 0.41
0.374TyrTrp: 0.374 ± 0.204
1.945TyrTyr: 1.945 ± 0.523
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13367 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski